The invention belongs to the field of cosmetics and discloses a stable oil-soluble polypeptide composition, comprising the following components: polypeptide, solubilizer, and oily solvent; the octanol-water partition coefficient of the solubilizer is 1.70-1.90 or the HLB value is 8.0 ‑10.0; the polypeptide is selected from palmitoyl dipeptide‑7, palmitoyl tripeptide‑1, palmitoyl tripeptide‑5, palmitoyl tripeptide‑8, palmitoyl tetrapeptide‑5, palmitoyl tetrapeptide‑7, At least one of palmitoyl tetrapeptide-10 and palmitoyl pentapeptide-4; the solubilizer is selected from ethylhexylglycerin, glyceryl caprylate, fatty acid polyoxyethylene ester, sorbitan laurate and poly At least one of oxyethylene oxypropylene oleate. The oil-soluble polypeptide composition of the present invention is easy to add to cosmetics with full oil formula, and has good compatibility. The oil-soluble polypeptide composition has good stability and is not easy to grow bacteria. Compared with traditional water-soluble polypeptide products, it does not need to add Preservatives, the formula is safer.