The invention discloses a preparation method of a medicament-carrying biological membrane, which comprises the following steps: preparing type I collagen into a porous and degradable collagen spongy carrier by a freeze-drying process by temperature cross-linking; according to the pharmacokinetics of the selected medicaments, adding medicaments with a certain concentration, such as antibiotics, antiepileptic medicaments, nerve growth factors, NMDA (N-methyl-D-aspartic acid) receptor stimulants, NMDA receptor antagonists and the like, into the liquid medicine slowly in the preparation process of slurry, or soaking a biological membrane into the liquid medicine with a certain concentration; adsorbing the medicaments for a period of time; freeze-drying; packaging; and sterilizing in a proper sterilizing mode (gamma rays or epoxy ethane) according to the properties of the medicaments to form a medicament-carrying preparation with a slow-release effect. The invention has wide application prospects in the technical fields of infection prevention, nerve growth promotion, NMDA metabolism, epileptic prevention and the like.