35 results about "Microbial biodiversity" patented technology

The invention discloses a lysinibacillus fusiformis and its application in ecological reconstruction of mine area reclamation. The bacterium is Lysinibacillus fusiformis, and its preservation number is CGMCC No.9799. The strain can increase the nutrient content of reclaimed soil in mining areas, and has a significant effect on increasing the content of organic matter, alkaline nitrogen, available potassium and available phosphorus in reclaimed soil in mining areas; and further experiments have shown that the spindle-shaped lysine bacillus It can also increase the biomass of crops, whether it is for the cultivation of wheat or rapeseed, it can increase the biomass of crops in reclaimed soil in mining areas; it is particularly important that the use of spindle-shaped lysine bacillus can effectively and significantly change soil microorganisms The community structure of the soil can improve the diversity of soil microorganisms, effectively increase the number of microorganisms, thereby speeding up the restoration of soil microbial communities, and can be widely used in the restoration of reclaimed soil in coal mining areas.

The invention discloses a bacillus subtilis HL-1 and an application thereof in the respect of soil phosphate dissolving. The strain is collected in China General Microbiological Culture Collection Center (CGMCC) on Aug 24th, 2011, with a collection number of CGMCC No.5175. The 16SrDNA nucleotide sequence of the strain is represented by SEQ ID NO:1. With added diatomite and guava slag adsorbing agent materials, a fermentation broth of the bacillus subtilis HL-1 can be prepared into microbial manure. With the strain provided by the invention, phosphate availability and phosphate fertilizer utilization rate of the soil can be improved, plant growth can be promoted, and soil microbe diversity can be improved. The strain has important significance in reducing chemical fertilizer application, modifying soil micro-ecology, and promoting soil sustainable utilization. The strain has wide application prospect in production practice.

The disclosure relates to improving plant breeding methods by controlling for microbial diversity present in the plant breeding process.

A method of interfering with quorum sensing regulation of genes to promote cell growth is disclosed. The method of is aimed at accessing microbial biodiversity. The method involves obtaining an environmental sample comprising at least one novel (uncultivated in the laboratory) microorganism, contacting the environmental sample with an effective amount of an agent or combination of agents which interferes with the quorum sensing regulation of genes, growing the treated sample in a culture medium containing the quorum sensing signal disrupting agent or agents, and analyzing the colonies of microorganisms grown to demonstrate genetic novelty.

The invention discloses a qualitative and / or quantitative extraction method of total DNA of large-grain substrate surface microorganisms in constructed wetlands. The method comprises the steps that large-grain substrates used for constructed wetlands filling serve as materials, biological membranes on the surfaces of the large-grain substances are eluted through a shaking table oscillation method, centrifugation is conducted to obtain precipitates, the precipitates are subjected to humic acid removing treatment with a humic acid removing buffer solution and a calcium chloride solution, cells are subjected to pyrolysis through an SDS-lysozyme method, chloroform extraction is conducted, and isopropanol precipitating is conducted to obtain DNA. Compared with an existing kit extraction method without pretreatment, the extracted total DNA of the substrate surface microorganisms in the constructed wetlands is high in concentration and purity and good in integrity, a small amount of information of microbial diversity is lost, and the method can be directly used for PCR, real-time quantitative PCR and DGGE experiment analysis. The method has the advantages of being low in extraction cost, short in consumed time and the like, the contamination rate in the sample treatment process is decreased, the DNA loss caused by freeze thawing is reduced, and the problems that when the total DNA of the large-grain substrate surface microorganisms in the constructed wetlands are extracted directly, the yield ratio is low, the purity is low and a small amount of the diversity information is obtained are solved.

The invention provides an intelligent quality detection method and system in logistic process of cold-chain meat and meat products. The detection method comprises steps as follows: analyzing and determining predominant decomposing microorganisms in samples, determining dynamic fluctuation temperature of the predominant decomposing microorganisms in each sample, setting culture temperature and a time parameter to put each sample in a fluctuation temperature incubator for cultivating, performing plate counts of total bacterial count and total predominant decomposing microorganisms count, establishing growth and survival curves under dynamic condition, constituting a three-stage dynamic model of microorganisms in cold-chain meat products, optimizing the kinetic parameter and obtaining intermittent dynamic prediction model of different predominant decomposing microorganisms under temperature fluctuation condition. The system can exactly and instantly describe microbial growth and survivalcharacteristics under fluctuation temperature condition, establishes an Internet and technology real-time visualization platform for the freshness of different cold-chain meat and meat products, so that intelligent logistics between cold-chain meat and meat products are realized, consumer confidence is improved, industry losses are reduced and economic benefit is improved.

The invention relates to a method for simultaneously improving sludge anaerobic digestion efficiency and reducing the ecotoxicity of heavy metals. According to the method, after straws are activated with a ZnCl2 solution, straw biochar is prepared under a 550 DEG C high-temperature anaerobic condition, and further modification is performed by using an HCl solution and microwave; and by using modified biochar as carrier, a zero-valent nano-iron loaded modified biochar composite material HCl-FBC is prepared through a chemical reaction. According to the present invention, by adding the modified biochar composite HCl-FBC to the anaerobic digestion reactor at the initial stage of sludge anaerobic digestion, the organic matter conversion rate of sludge can be significantly increased, and the cumulative gas production of methane can be increased; HCl-FBC can easily increase the microbial diversity and the activity of the digested sludge and change the microbial community structure; and with the method, the sludge anaerobic digestion efficiency can be significant increased, the biogas production can be increased, the biological toxicity of the heavy metal in the digested sludge is effectively reduced, and the great significance is provided in the resource utilization of sludge and the reduction of the risk of sludge land use.

The invention relates to an interplanting method for covering gardenia with grass and increasing efficiency, and belongs to the technical field of agricultural planting. The invention discloses an interplanting method of gardenia mulch with grass and synergistic effect. Black beans and gardenia are interplanted, and after the interplanting, the coverage rate of the bottom surface is increased without affecting the light of gardenia, and at the same time, the physical and chemical properties of soil can be improved by using black beans , Improve soil nitrogen content, increase microbial diversity and rich organic matter content, reduce the use of herbicides, solve the problems of microbial imbalance in gardenia planting land caused by herbicide application, improve the growth environment of gardenia, reduce gardenia garden input, increase income, and finally achieve the purpose of covering gardenia with grass and increasing efficiency.

The invention discloses enterobacter 3bh19 for improving the microecology of the cucumber leaf circumference to prevent and control downy mildew and its application. The biocontrol bacterium 3bh19 is Enterobacter sp. The bacteria were prepared at a concentration of 1×10 8 For the bacterial agent with a concentration above CFU / mL, under the normal management conditions of the greenhouse, the biocontrol effect of Enterobacter 3bh19 microbial agent on the cucumber downy mildew after spraying treatment reached more than 63.32%; and the biomass of cucumber plants on the 30th day after treatment Compared with the control, it has increased by more than 32%; in the field, the control effect on various cucumber downy mildews is more than 53.55%, and the growth-promoting effect is very significant; and it can significantly improve the stress resistance of plants, and can significantly improve the microorganisms around the leaves of plants Diversity, improved microecology.