Preparation method and application of litter leach liquor adding culture medium of soil microorganisms

Abstract

The invention discloses a preparation method and application of a litter leach liquor adding culture medium of soil microorganisms. The preparation method is characterized in that a typical litter under a plant biotope where investigated soil is positioned is selected, about 1kg of multi-point mixing samples of an un-decomposed layer under the surface layer of the litter is directly collected andis packaged in a plastic bag to be taken back to a laboratory, a pair of scissors is adopted to cut the litter into pieces, the litter and distilled water are mixed in a triangular flask according toa mass ratio of 1:15, after a mixture is vibrated by a table concentrator for 30min, standing is carried out for 10min, the obtained mixture is filtered by gauze, and filtrate is litter leach liquor.Under a situation that the rest medicines in a formula of a bacterium, fungus and actinomycetes culture medium in the existing soil microorganisms are constant, the litter leach liquor is used for replacing 10% of a use amount of distilled water, and after high-temperature sterilization is carried out, the culture medium is obtained. The preparation method disclosed by the invention has a low cost, can be conveniently manufactured, can obviously improve the diversity of culturable microorganisms, and provides technical support for isolated culture and subsequent relevant research of the soil-culturable microorganisms.

Description

technical field [0001] The invention belongs to the technical field of soil microorganism cultivation and separation research, and specifically relates to the preparation and application of a soil cultivable microorganism culture medium, and also relates to the extension research design in the soil microorganism cultivation and separation research. Background technique [0002] Microbes are an important biological component of soil and an important driving force for the geochemical cycling of elements in soil ecosystems. The biodiversity of soil microorganisms is very rich. Although the current use of microbial non-culture technologies such as high-throughput sequencing technology can help us understand the composition and diversity of soil microbial communities, due to the lack of cultured and isolated soil microorganisms 1% of the actual species abundance in nature, which greatly restricts people's in-depth understanding of the biological characteristics and ecological fun...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current studies on the diversity of soil microorganisms using culture methods, most of them simply choose to use relatively mature medium formulas, ignoring the problem of exploring and researching the improvement of the culture medium formula to increase the number of soil microorganisms that can be cultivated

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