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A spindle-shaped lysine bacillus and its application in ecological reconstruction of mining area reclamation

A lysine bacillus and spindle-shaped technology is applied to the spindle-shaped bacillus lysine and its application field in the ecological reconstruction of mining area reclamation, which can solve the problems of poor soil structure in reclamation and long reclamation period.

Active Publication Date: 2021-06-08
SHANXI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages of long reclamation period and poor soil structure in existing mining areas, and provide a spindle-shaped lysinic bacillus with functions of decomposing phosphorus, potassium and nitrogen and its application in mining areas. The application in reclamation ecological reconstruction can effectively improve the nutrient level and microbial community of reclaimed soil in mining areas, promote crop growth, and speed up ecological reconstruction

Method used

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  • A spindle-shaped lysine bacillus and its application in ecological reconstruction of mining area reclamation
  • A spindle-shaped lysine bacillus and its application in ecological reconstruction of mining area reclamation
  • A spindle-shaped lysine bacillus and its application in ecological reconstruction of mining area reclamation

Examples

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Effect test

Embodiment 1

[0030] This example illustrates the screening and identification process of Lysinibacillus fusiformis (CGMCC No. 9799) of the present invention

[0031] 1. Sample Collection

[0032] Soil was collected from a typical reclamation mining area in Shanxi Province, and 0-20 cm soil was collected from the weeds (N 36°28′17.846″, E 113°00′50.383″) in the coal mining subsidence reclamation area of ​​Xishandi Village, Xiangyuan County, Changzhi, Shanxi Province. The samples were stored at 4°C; 0-20 cm soil samples were collected from the weeds (N 37°08′25.485″, E 111°30′65.453″) of the Ke'e Village dump in Xiaoyi, Shanxi Province, and were stored at 4°C.

[0033] 2. Primary screening of strains

[0034] Weigh 10g of the collected reclaimed soil from the mining area, add it to 90mL of a sterilized 250mL conical flask containing glass beads and sterile water, shake it for 20min under a constant temperature shaker at 30°C and 180r / min, and absorb 1mL of bacteria after standing for 5min. ...

Embodiment 2

[0071] This example illustrates the cultivation of Bacillus fusiformis and the preparation of microbial inoculants of the present invention

[0072] A fusiform lysine bacillus microbial preparation is the active ingredient obtained by culturing the fusiform lysine bacillus in a mass ratio of 2:10-20 after expanding the culture, and a carrier is fully mixed, and stored at room temperature;

[0073] The carrier is a solid carrier or a liquid carrier, wherein the solid carrier is a mineral material, a plant material, a polymer compound or an organic material; the mineral material is one of clay, kaolin, montmorillonite, zeolite and fly ash or The plant material is one or more of corn flour, starch, soybean flour and peanut flour; the macromolecular compound is one or both of polyvinyl alcohol and polyglycol; the organic material is One or both of peat, humic acid and organic fertilizer;

[0074] The active ingredient is one or more of the living cells obtained by expanding the c...

Embodiment 3

[0080] This example illustrates the effect of the spindle-shaped lysine bacillus agent of the present invention on improving the nutrient content of the reclaimed soil in the mining area

[0081] Rapeseed rape and spring wheat were grown by pot method, and the planting time was (60 days and 100 days) respectively. The substrate soil used for potted plants comes from the reclaimed land in the coal mining subsidence area of ​​Lu'an Group, located in the southeast of Shanxi Province, at the western foot of Taihang Mountain.

[0082] Rape soil organic matter content 5.97g / kg, total nitrogen 0.50g / kg, total phosphorus 0.05g / kg, total potassium 20.00g / kg, alkaline hydrolyzable nitrogen 42.82mg / kg, available phosphorus 3.39mg / kg, available potassium 98.60mg / kg, pH 8.29.

[0083] Spring wheat soil organic matter content 6.40g / kg, total nitrogen 0.40g / kg, total phosphorus 0.4g / kg, total potassium 19.80g / kg, alkaline hydrolyzable nitrogen 48.76mg / kg, available phosphorus 2.09mg / kg, av...

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Abstract

The invention discloses a lysinibacillus fusiformis and its application in ecological reconstruction of mine area reclamation. The bacterium is Lysinibacillus fusiformis, and its preservation number is CGMCC No.9799. The strain can increase the nutrient content of reclaimed soil in mining areas, and has a significant effect on increasing the content of organic matter, alkaline nitrogen, available potassium and available phosphorus in reclaimed soil in mining areas; and further experiments have shown that the spindle-shaped lysine bacillus It can also increase the biomass of crops, whether it is for the cultivation of wheat or rapeseed, it can increase the biomass of crops in reclaimed soil in mining areas; it is particularly important that the use of spindle-shaped lysine bacillus can effectively and significantly change soil microorganisms The community structure of the soil can improve the diversity of soil microorganisms, effectively increase the number of microorganisms, thereby speeding up the restoration of soil microbial communities, and can be widely used in the restoration of reclaimed soil in coal mining areas.

Description

technical field [0001] The invention belongs to the technical field of ecological reconstruction, and relates to a spindle-shaped Bacillus lysine and its application in the ecological reconstruction of mining area reclamation. Background technique [0002] Coal plays an important role in my country's energy production and consumption, and its proportion has always remained at about 70%. While supporting social and economic development, coal resource mining also has a lasting and serious negative impact on land resources and ecological environment. "2007 China Geological Environment Bulletin" pointed out that the land area occupied and damaged by mining development nationwide is 1.658 million hectares, including 909,000 hectares of tailings, 522,000 hectares of open pits, 203,000 hectares of mining subsidence, and new damages are added every year. The land is about 4 million mu, of which more than 60% is arable land or other agricultural land, resulting in the intensifying co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C09K17/14C12R1/01
CPCC05F11/08C09K17/14C12N1/20C12N1/205C12R2001/01
Inventor 李建华郜春花张强李华靳东升卢晋晶李娓
Owner SHANXI UNIV
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