Enterobacter 3bh19 and its application to improve the microecology of cucumber leaf circle to control downy mildew

A technology of Enterobacteriaceae and Enterobacter strains, applied to Enterobacter 3bh19 and its application fields, can solve the problems of farmers' economic loss, reduced yield, and delayed fruit development, and achieve the effect of protecting the environment and promoting sustainable development.

Active Publication Date: 2019-09-13
镇江蔚绿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the investigation of the disease around Beijing, Shandong, Hebei and other places, it was also found that the disease caused serious damage. In the greenhouses where the disease was serious, the rate of diseased leaves reached more than 85%, the fruit growth was delayed, and the yield was reduced, which caused great harm to farmers. big economic loss

Method used

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  • Enterobacter 3bh19 and its application to improve the microecology of cucumber leaf circle to control downy mildew
  • Enterobacter 3bh19 and its application to improve the microecology of cucumber leaf circle to control downy mildew
  • Enterobacter 3bh19 and its application to improve the microecology of cucumber leaf circle to control downy mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Molecular Identification of Enterobacter 3bh19

[0025] Bacterial Genome Kit (Saibaisheng Company) After extracting bacterial gene DNA, use gyrB universal primers gyrB1 (5'-GAAGTCATCATGACCGTTCTGCAYGCNGGNGGNAARTTYGA-3'); gyrB2R (5'-AGCAGGATACGGATGTGCGAGC CRTCNACRTCNGCRTCNGTCAT-3') to amplify gyrB gene from genomic DNA fragment. The specific amplification system and procedure (25ul) are as follows: 1 μl each of primers 1 and 2 (10 μM); Mix: 12.5 μl; Taq: 0.5 μl. wxya 2 O: 10 μl; template: 1 μl. Pre-denaturation at 94°C for 5min, denaturation at 94°C for 1min, annealing at 56°C for 2min, extension at 72°C for 2min, 35 cycles, extension at 72°C for 10min, storage at 4°C. PCR amplified products were detected by 1% agarose gel electrophoresis, 90V, 30min; EB stained for 30min, and amplified products were detected. After purification, the amplified product was connected to the pMD19-T vector, transformed into Escherichia coli DH5α, and the positive clone plasmid ...

Embodiment 2

[0027] An Enterobacter 3bh19 that improves the biological control effect of cucumber downy mildew, promotes growth, resists stress, and improves microecology, and contains microbial components: Enterobacter 3bh19 (preservation number is CGMCC NO.12608). It was isolated from the rhizosphere soil of Huai'an cucumber.

[0028] The preparation method of Enterobacteriaceae 3bh19 bacterial agent is as follows:

[0029] Enterobacter 3bh19 was cultured in LB culture medium at 28°C and 180rpm for 12-16h, then centrifuged at 6000rpm for 10min to obtain the bacterial cells, and then diluted the bacterial cells with sterilized water to make bacterial preparations. The total concentration of viable bacteria in the finished bacterial preparations was 1× 10 9 -1×10 10 CFU / mL.

Embodiment 3

[0030] Example 3 Greenhouse Growth-promoting Test of Enterobacter 3bh19 on Cucumber

[0031] Method: soak the cucumber seeds with 10% H 2 o 2 The surface was disinfected for 10 minutes, washed with sterile water and dried for later use. Sow 10 seeds per nutrient bowl. After 4 weeks of normal watering management, the seedlings with consistent growth were transplanted into 330ml plastic cups, and the suspended Enterobacter 3bh19 bacterial agent was mixed with 1.0×10 7The concentration of CFU / mL is applied to the roots of crops by irrigation, 30mL per plant. Then placed in a greenhouse for cultivation, 28°C, 70% relative humidity. The water treatment was used as the control, and 48 seedlings were treated in each group, placed randomly, and repeated three times. After 30 days, the plant height, stem diameter, fresh weight, dry weight and other biomass of cucumber plants were measured.

[0032] Table 1 Effect of Enterobacter 3bh19 on cucumber biomass

[0033]

[0034] Not...

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Abstract

The invention discloses enterobacter 3bh19 for improving the microecology of the cucumber leaf circumference to prevent and control downy mildew and its application. The biocontrol bacterium 3bh19 is Enterobacter sp. The bacteria were prepared at a concentration of 1×10 8 For the bacterial agent with a concentration above CFU / mL, under the normal management conditions of the greenhouse, the biocontrol effect of Enterobacter 3bh19 microbial agent on the cucumber downy mildew after spraying treatment reached more than 63.32%; and the biomass of cucumber plants on the 30th day after treatment Compared with the control, it has increased by more than 32%; in the field, the control effect on various cucumber downy mildews is more than 53.55%, and the growth-promoting effect is very significant; and it can significantly improve the stress resistance of plants, and can significantly improve the microorganisms around the leaves of plants Diversity, improved microecology.

Description

technical field [0001] The invention belongs to the field of plant protection, and relates to enterobacter 3bh19 for improving the microecology of cucumber leaf circles to prevent and control downy mildew and its application. Background technique [0002] Cucumber (Cucums sativus L.) belongs to Cucurbitaceae (Cucurbitaceae) and the genus Cucumis (Cucums) in plant taxonomy. According to statistics from the Food and Agriculture Organization of the United Nations, from 2000 to 2004, the cultivation area of ​​cucumbers in my country increased from 116,549 hectares (hm 2 ) increased to 1502 900 hectares (hm 2 ), production increased from 19 869 181 million tons (Mt) to 25 558 000 million tons (Mt). With the prevalence of greenhouses in field facilities, the cultivation methods of cucumbers have undergone tremendous changes. According to incomplete statistics, at present, most cucumbers in my country are grown in greenhouses in field facilities, which not only brings huge econo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 罗玉明张丽娜杨威郑丽
Owner 镇江蔚绿生物科技有限公司
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