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60 results about "Matrix cell" patented technology

The cell matrix is believed to be involved in cell shape determination and locomotive mechanism, particularly across a solid substrate.

Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria

Bacteria which co-express protease inhibitors and protease sensitive therapeutic agents, which are surface displayed, secreted and / or released and result in their localized production and maintenance within a target tissue and inactivation outside of the target tissue, thereby increasing therapeutic activity and reducing the systemic toxicity. The bacteria may be attenuated, non-pathogenic, low pathogenic or a probiotic. Protease sensitivity may be further accomplished by engineering protease degradation sites within the therapeutic agents, further enhancing the inactivation outside of the target tissue while retaining activity within the target tissue through co-expression of a protease inhibitor. Novel chimeric proteins secreted by bacteria, including chimeric toxins targeted to neoplastic cells, tumor matrix cells and cells of the immune system, and combination therapies of these protease inhibitor:chimeric toxin-expressing bacteria together with small-molecule and biologic agents are also described. Non-conjugative bacteria limiting exchange of genetic material, and antibody resistant bacteria are also provided.
Owner:BERMUDES DAVID GORDON

Whole layer biological cornea as well as construction method and use thereof

The invention aims at providing a novel full-thickness biological cornea used for transplantation. The full-thickness biological cornea takes an animal cornea acellular matrix as a carrier, and the acellular matrix comprises animal cornea matrix cells, epithelial cells and endothelial cells which are cultured and augmented in vitro. The cornea is characterized in that the xenogenic corneal acellular matrix prepared by a biochemical method can be used as a good carrier for in vitro constructing the biological cornea in the aspects of shape, structure and biological compatibility, the matrix cells, epithelial cells and endothelial cells of the cornea are planted respectively, and dynamically cultured in the simulated in vivo environment of a biological reactor, so that the full-thickness biological cornea with nearly normal tissue structure and characteristics can be constructed in vitro, and the biological cornea can be used to simulate the physiological cornea for fundamental research on physiology, pathology and pharmacology; moreover, the biological cornea can also be directly used as the donator for corneal transplantation.
Owner:SHANGHAI NINTH PEOPLES HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Use Of Umbilical Cord Matrix Cells

The invention relates to the isolation and use of stem cells from amniote species (potentially any animal with an umbilical cord, including humans). More particularly the invention relates to obtaining stem cells that are at least multipotent and may be totipotent or nearly totipotent and are envisaged to have a variety of end uses. The cells of the present invention are immunosuppressive and may be used to inhibit the immune response in a subject.
Owner:KANSAS STATE UNIV RES FOUND

Method for analysis of single pulse pressure waves

InactiveUS20070276264A1Optimal single pressure wave detectionHigh pressureEvaluation of blood vesselsCatheterPressure amplitudeWave parameter
This invention relates to a method for analysing pressure-signals derivable from pressure measurements on or in a body of a human being or animal, comprising the steps of identifying during given time sequences in a series of time sequences the single pressure waves, including related parameters [pressure amplitude ΔP, latency (ΔT), rise time coefficient (ΔP / ΔT)], determining numbers of single pressure waves with pre-selected combinations of two or more of said single pressure wave parameters during said time sequence. For the time sequences is further determined the balanced positions of single wave parameters. Two-dimensional values of balanced position may be presented as a one dimensional value after weighting of the matrix cells. The signal processing method may be used for more optimal detection of single pressure waves by means of non-invasive sensor devices.
Owner:EIDE PER KRISTIAN

Method for differentiating induced pluripotent stem cell into hepatocyte through directed induction, and hepatocyte thereof

The invention relates to a method for differentiating an induced pluripotent stem cell into a hepatocyte through directed induction, and the hepatocyte thereof. The method is characterized in that different media are added in different stages of directed induction differentiation to carry out directed induction differentiation of the induced pluripotent stem cell. The change of the form of the cell can be momentarily observed in the operating process to ensure normal implementation of induction, so a required liver precursor cell can be fast and efficiently obtained through induction. The method for differentiating the induced pluripotent stem cell into the hepatocyte through directed induction adopts a culture system of feeder-free cells, the hepatocyte can be obtained only through adding an induction medium in a corresponding stage, and a detection result shows that the purity is high. The method for differentiating the induced pluripotent stem cell into the hepatocyte through directed induction has the advantages of short induction period, high efficiency, stable performances and mature functions of the obtained hepatocyte, and no matrix cell pollution.
Owner:GUANGXIU-GAOXIN LIFE SCI CO LTD HUNAN

Chronic glaucoma animal model establishing method and application thereof

The invention discloses a chronic glaucoma animal model establishing method. The method comprises the following steps: (1) collecting conjunctiva tissues of GFP-SD rats, sterilizing, digesting, separating a conjunctiva epithelial layer, and obtaining a conjunctiva matrix tissue block; (2) digesting the conjunctiva matrix tissue block in pancreatin, terminating the digestion by using a cell culturesolution, blowing and beating to form a single-cell suspension, filtering by virtue of a cell sieve, centrifuging, and removing supernatant, thus obtaining conjunctiva matrix cell precipitates; (3) adding an appropriate amount of cell culture solution, blowing and beating to form single cells, counting, performing the in-vitro culture, and obtaining a remarkable amount of conjunctiva matrix cells; (4) digesting the conjunctiva matrix cells by using pancreatin, processing by utilizing the method of the step (2), obtaining the conjunctiva matrix cell precipitate, adding an appropriate amount ofcell culture solution, and obtaining cell suspension; and (5) injecting 10 micro liter of cell suspension by utilizing a micro injector into the anterior chamber of the right eye of a rat, and sufficiently and uniformly mixing. A glaucoma animal model obtained by using the method has the advantages of long-term stability, simplicity, ease, good repetition, and low cost.
Owner:XIAMEN EYE CENTER OF XIAMEN UNIVERSITY CO LTD

Micro-fluidic chip for cell migration and preparation method of micro-fluidic chip

The invention discloses a micro-fluidic chip for cell migration and a preparation method of the micro-fluidic chip. The micro-fluidic chip is provided with a flow layer, a control layer, a film layerand a glass layer from top to bottom; the flow layer consists of a pyramid-shaped network structure, a cell culture zone, a cell migration zone, a main pipeline and a liquid inlet micro pipeline; andthe control layer consists of a plurality of micro valves. The invention also provides a preparation method of the micro-fluidic chip. A biochemical factor / medicine concentration gradient is formed inthe main pipeline and the cell migration area, the effect of the biochemical factors / medicine of different concentrations for the cell migration can be researched in a same chip, and the conventionalmode for researching the effect of the single-concentration biochemical factor / medicine for the cell migration is broken through. The invention can also realize the environment construction of an extracellular matrix and co-culture of target cells and matrix cells, and the micro-fluidic chip is used for researching the haptotaxis of the extracellular matrix and the matrix cell for the target cell. Therefore, the cell migration can be researched by combining the biochemical factor / medicine concentration gradient and cell micro environment simulation.
Owner:NORTHWESTERN POLYTECHNICAL UNIV

Intelligent scheduling and tracing system and method for power battery formation process

The invention relates to the technical field of power batteries, and discloses an intelligent scheduling and tracing system and method for a power battery formation process, so as to realize scheduling intelligence and product tracing convenience in a production process. The system comprises a code printing and tray filling machine for printing battery codes; a battery container tray which is provided with a readable and writable RFID chip and matrix cells; a central server; a reader-writer for reading RFID chip information of the battery container tray which is arranged in each clamping groove structure of the formation cabinet; after the battery container tray is placed in the formation cabinet, the edge computing server controls the formation cabinet to perform a normal charging and discharging process according to the initialized production process, and records the charging and discharging data of each battery in system traceability associated information; the associated information further comprises RFID information of each battery code and the corresponding tray, coding information of corresponding cells in the tray and point location information of the formation cabinet; the system further comprises a sorting device for sorting the batteries according to the capacity sections based on the RFID chip information.
Owner:CENT SOUTH UNIV +1

LED display microarray and preparation method thereof

The invention relates to an LED display microarray and a preparation method thereof and belongs to the technical field of semiconductor illumination. The LED display microarray comprises a substrate epitaxial wafer, a semiconductor matrix isolation region, an insertion layer, an electronic conducting layer, a light-emitting layer, a hole conducting layer, an electronic conducting electrode, a hole conducting electrode, an isolation protecting layer, anode lines and cathode lines. An epitaxial layer is subjected to dry etching so that the electronic conducting layer and a semiconductor matrix cell array are obtained; the electronic conducting layer to the insertion layer is subjected to dry etching so that isolation of each two adjacent cells is achieved, and the semiconductor matrix isolation region is obtained; the electronic conducting electrode is formed to lead the cathode lines out; the isolation protecting layer is deposited; the hole conducting electrode is formed to lead the anode lines out; and the spatially intersectional regions of the anode lines and the cathode lines are display pixels. By the LED display microarray and the preparation method, the insertion layer is grown epitaxially to achieve isolation, the problem of electricity leakage caused by process difficulty in achievement of deep isolation trenches by a dry etching method is reduced, the depth of isolation trenches is decreased, and cost is lowered.
Owner:BEIJING UNIV OF TECH
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