56 results about "IMP dehydrogenase activity" patented technology

The invention provides a method for screening stereoselective alpha-hydroxy acid dehydrogenase, comprising the following steps: (1) dissolving a sample to be detected containing alpha-hydroxy acid dehydrogenase in a buffer with pH of 6-9, adding an alpha-hydroxy acid chiral monomer as a substrate, and carrying out conversion reaction in water-bath at 20-50 DEG C; and (2) after the conversion reaction, adding the conversion solution in an FeCl3 developer solution to conduct color development reaction for 5-30 min, when the color development reaction finishes, judging the result according to the color appeared in the reaction solution. The method has no need to carry out derivatization on the substrate which is intend to screen, can rapidly identify the dehydrogenase activity and optical selectivity of the selected microbe by using simple colourimetry, has the advantages of simple screening flow, fast speed, low request for devices, strong versatility and the like, and offers great conveniences to the obtainment of optically pure products by using racemic mandelic acid and related derivatives as substrates to carry out resolution through biological enzymatic method.

Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L-erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D-gluconate dehydrogenase activity, a D-mannuronate hydrogenase activity, and / or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and microbial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.

The invention relates to the field of microorganisms and discloses a microbial strain capable of remedying heavy metal-contaminated soil and application of the microbial strain. The preservation number of the microbial strain is CGMCC No.10843. A strain of burkholderia cepacia is screened from the soil contaminated by heavy metal cadmium and can purify the exchangeable cadmium in the soil and significantly reduce the content of the exchangeable cadmium. The microbial strain is extremely-strong in tolerance to the cadmium and small in soil disturbance, has a stronger biological remediation function, meanwhile can improve the activity of soil dehydrogenase and can also be applied to the remediation of the heavy metal-contaminated soil and the improvement of the activity of the soil dehydrogenase as well as the preparation of relevant products.

The invention provides a 3-sterone-Delta[1]-dehydrogenase. By constructing various expression vectors and converting relevant strains, the invention obtains various engineering strains such as non-3-sterone-Delta[1]-dehydrogenase activity strains or high 3-sterone-Delta[1]-dehydrogenase activity strains; the engineering strains can be adopted to selectively prepare androst-4-alkene-3, 17-diketone and 9Alpha-androst-4-alkene-3, 17-diketone or androst-1, 4-diene-3, 17-diketone and 3-ketone-1, 4-dienes steroid compounds. The engineering strains can greatly improve the production efficiency and product quality of the steroid medical production system, are beneficial for reducing the energy consumption of steroid drugs during the production process, improving the utilization ratio of the prodrugs and simplifying the production steps, reduce the production cost, have moderate reaction conditions and friendly environment, are suitable for wide generalization and application and have higher economic benefits and social benefits.

The invention provides a dehydrogenase activity detection pretreatment method used in drinking water biological active carbon treatment technique, which is a mixed solution composed of three kinds of reagents, under the control of adding proportionment, adding time and operational condition, the pretreatment is finished, thereafter the measuring process is selected and the specification curve is drawn. The invention has the advantages that, from the chemical angle, using organic reagent to extract and elute the adsorbed microorganism, the desorption degree is high; testing the active carbon carried dehydrogenase activity with effective proportioning and oscillation mode, and confirming the optimum organic solvent combination and proportioning for achieving the maximum measuring effect; providing the reference for similar biological carrier activity test, which is suitable for biological activity test of biological carrier in drinking water treatment technique.

The present invention provides a method for producing L-fuculose and L-fucose which is suitable as an industrial method. L-Fuculose is synthesized from L-fucitol in the presence of a microorganism-derived protein having a dehydrogenase activity which results in production of L-fuculose from L-fucitol. The reaction system preferably contains NADH oxidase. L-Fuculose thus synthesized is then converted into L-fucose.

The invention discloses a TTC- dehydrogenase activity testing method of drinking water, it relates to a environment monitoring method, especially a monitoring method to test biology activity for drinking water treatment. Nowadays, the method of testing biology activity of sewage biology treatment, because the solvent is vaporized, it is easily to break the stopper and overflow, so it leads to unstable result, so the biochemical analysis technology is limited to use in actual application. The method includes the following operation steppes: a. sample collecting and preparing, b. sample concentration, c. sample culturing, d. finishing enzyme reaction, e. color imetric analysis. The invention solves the biology activity testing problem under testing measure and low matrix concentration conditions, its result is stable, and has very high application value, it is propitious to be popularized and applied.

L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.

Disclosed is a mutant fructosyl amino acid oxidase modified at an amino acid residue involved in proton relay system. The mutant fructosyl amino acid oxidase has reduced oxidase activity while substantially maintaining its dehydrogenase activity. The invention also provides an assay device and assay method for measuring glycated protein.

This invention provides systems and methods for the increased production of ethanol and other chemical compounds by recombinant Clostridium species whereby the recombinant species are genetically-engineered to disrupt lactate dehydrogenase activity and to hydrolyze and ferment carbonaceous biomass and synthesize compounds of commercial value without production of lactic acid.

The present invention belongs to the field of microbe activity measuring technology, and is especially the measuring method of sludge dehydrogenase activity for biological waste water treatment. The measuring method includes three work processes of conventional sample preparation, standard curve making and sample measurement. In standard curve making process, the reductant si 10-20 % concentration Na2S.9H2O solution; and in sample measurement process, mixed liquid compounded in conventional method is first cultured, then separated and added with acetone to extract TF, and finally mixed and color compared. Compared with available technology, the present invention has simple standard curve making, raised TF stability and accuracy, high reliability, and other advantages.

The invention discloses dual purposes of linoleate isomerase in aspects of dehydrogenation and isomerism. A bifunctional enzyme PAI is used as delta-12 fattyaciddehydrogenase and linoleate isomerase or delta-12 fattyaciddehydrogenase; and the bifunctional enzyme PAI is protein a) or protein b), wherein the protein a) has amino acid sequences shown as a sequence 2 in a sequence table; and the protein b) has an amino acid residue sequence which is derived from the sequence 2 by substituting and / or losing and / or adding one or more amino acid residues, and has the activity of the delta-12 fattyaciddehydrogenase and linoleate isomerase or the delta-12 fattyaciddehydrogenase. The invention provides a basis and a method for further researching pai gene functions and performing related applicability research on the biosynthesis of t10c12-CLA (conjugated linoleic acid) by using the bifunctional enzyme PAI.

By reacting inosinic acid (IMP) with a bacterium which has been modified so that IMP dehydrogenase activity and 5′-guanylic acid (GMP) synthetase activity are enhanced, GMP is produced.

The invention relates to the technical field of ethanol detection of blood serum and in particular relates to an enzyme-method ethanol (ALC) detection reagent with a high anti-interference capability and high accuracy. A reagent R1 is mainly prepared from a buffering solution, ammonium oxalate, hydrazine hydrochloride, potassium nitroprusside, an ion balancing agent, ascorbic acid oxidase, a protecting agent, a surfactant, NAD (Nicotinamide Adenine Dinucleotide) and a preservative; a reagent R2 is mainly prepared from a buffering solution, a protecting agent, a heavy meal ion chelating agent, alcohol dehydrogenase and a preservative. The ammonium oxalate and the hydrazine hydrochloride are added into the reagent R1 so that interference caused by lactic dehydrogenase and hydroxybutyrate dehydrogenase can be effectively removed; less potassium nitroprusside is added so that interference caused by reducing substances including bilirubin and the like is effectively removed, so that the anti-interference capability of the reagent is greatly improved; the ascorbic acid oxidase is added so that interference caused by ascorbic acid can be effectively removed; the heavy metal ion chelating agent aminotriacetic acid is added so that interference caused by heavy metal ions, especially calcium ions, on dehydrogenase activity is removed very well, so that the activity of the alcohol dehydrogenase is improved, and the reagent becomes one detection reagent with the high anti-interference capability and the high accuracy.

The invention discloses a Mycoplasma bovis alcohol dehydrogenase gene, having a nucleotide sequence shown as SEQ ID NO: 1. The invention also discloses a protein coded by the Mycoplasma bovis alcohol dehydrogenase gene; the protein has an amino acid sequence shown as SEQ ID NO: 2 and belongs to the technical field of prevention and treatment of animal infectious diseases and biology. The protein, as a recombinant protein herein, has alcohol dehydrogenase activity, can attach to EBLs (embryonic bovine lung cells), can combine with bovine Fn (fibronectin), has good immunogenicity and reactogenicity, is a virulence-related protein of Mycoplasma bovis, and has a good application prospect in the research on pathogenesis of Mycoplasma bovis, and the research and development of vaccines and diagnostic reagents.

By reacting inosinic acid (IMP) with a bacterium which has been modified so that IMP dehydrogenase activity and 5′-guanylic acid (GMP) synthetase activity are enhanced, GMP is produced.

The invention relates to a method for quickly screening filamentous fungi for producing polyunsaturated fatty acid. The method is characterized in that a fatty acid synthase inhibitor cerulenin is used as a resistance screening factor, triphenyltetrazolium chloride characterizing the activity of dehydrogenase is used as a screening indicator, and a method for quickly and efficiently screening the filamentous fungi for producing the polyunsaturated fatty acid at high yield from environment is established. The method disclosed by the invention is simple and quick in process; the high-yield filamentous fungi is efficiently screened, and great convenience is provided for screening and researching filamentous fungi for producing oil; meanwhile, the invention provides a high-efficiency screening method and a batch of effective original strains for producing the polyunsaturated fatty acid by utilizing microbial fermentation.

The invention discloses a preparation method of a biological activated carbon filter. The method comprises a test process. The test process comprises pre-ozonation and coagulation, settling, filteringand ozone-biological activated carbon, wherein conventional processing parameters are basically constant, that is, processing water volume is 3-3.5m<3> / h, mixing time is 6-6.5s, reaction time is 23.2-19.9min, an ascending flow rate of a settling tank clear water area is 1.39-1.62mm / s, an ascending flow rate in an oblique tube is 1.60-1.87mm / s, a filter rate of the filter is 6.49-7.57m / h, a coagulant and a pH regulating agent are respectively liquid alkaline aluminum and sodium hydroxide, feeding concentrations are respectively around 2.5mg / L and 6mg / L, and then, the biological activated carbon filter is obtained. A detection method of the filter involves ammonifying bacteria, nitrite bacteria, nitrifying bacteria, heterotrophic bacteria, and dehydrogenase activity. The preparation methodhas the advantages of eliminating organic matters and polluted matters in feed water of a carbon filter of a water supply factory and purifying the water. Compared with other sewage deep processing technologies, the biological activated carbon filter prepared by the method has a simple structure, occupies a small space, and runs conveniently.

Fermentation processes for production of ethanol include supplying a thermophilic microorganism lacking lactate dehydrogenase activity with sugars under conditions in which they metabolise them predominantly by the pyruvate-formate lyase pathway. Importantly, the processes also include supplying sufficient glycerol to convert all of the sugars to ethanol. A further embodiment of the invention includes supplying additional glycerol sufficient to convert the exogenous acetate present in biomass hydrolysates into ethanol. Any type of fermentation system can be used for these processes, but a preferred embodiment includes continuous cultures at high temperatures in which ethanol is removed continuously by vacuum evaporation.

Compositions, devices, kits and methods are disclosed for assaying cholesterol with a cholesterol oxidase mutant that has been modified at an amino acid residue involved in the active site. The cholesterol oxidase mutant has reduced oxidase activity while substantially maintaining its dehydrogenase activity.

A reagent for measuring an alanine aminotransferase activity comprising L-alanine, 2-oxoglutaric acid, lactate dehydrogenase, and reduced nicotinamide adenine dinucleotide, characterized by further comprising a substance having an activity of inhibiting lactate dehydrogenase activity is disclosed. Further, a method for measuring an alanine aminotransferase activity, characterized by bringing a sample to be analyzed, which may contain alanine aminotransferase, into contact with L-alanine, 2-oxoglutaric acid, lactate dehydrogenase, reduced nicotinamide adenine dinucleotide, and a substance having an activity of inhibiting a lactate dehydrogenase activity is disclosed. According to the reagent and method, an increase in the reagent blank reaction, i.e., an increase in the initial absorbance, can be suppressed.

The invention belongs to the technical field of microbial remediation of organic polluted soil, and in particular relates to a petroleum hydrocarbon degrading mixed inoculum suitable for a medium-temperature aerobic biological heap remediation system and applications of the petroleum hydrocarbon degrading mixed inoculum. The mixed inoculum is prepared in a manner that bacillus licheniformis and pseudomonas fluorescens are mixed and immobilized, wherein bacillus licheniformis and pseudomonas fluorescens are mixed at the volume ratio of (3:1) to (5:1), a rice hull carrier after acidification andneutralizing of the pH is adopted for carrying out immobilization, and thus the mixed inoculum is prepared. When the total amount of bacterial microbes in the biological heap achieves 40-60 % of theinitial content, the mixed inoculum is supplied during the interval of stopping of forced ventilation of the heap, the total amount of bacteria in the heap is maintained to be 90-110 %, and meanwhile,the activity of dehydrogenase in the polluted soil is enabled to be 70-90 % of the initial value, so that the biological heap efficient remediation process of the petroleum-polluted soil is effectively realized.

A microorganism capable of producing acrylic acid, comprising a genetic modification that increases activity of CoA acylating aldehyde dehydrogenase (ALDH) catalyzing conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-hydroxy propionyl-CoA (3-HP-CoA) and a genetic modification that increases activity of 3-HP-CoA dehydratase catalyzing conversion of 3-HP-CoA to acrylyl-CoA in the microorganism in comparison with a cell that is not genetically engineered; as well as a method of producing the microorganism, and a method of producing acrylic acid using the same.

Described herein are host cells comprising lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and / or n-propanol dehydrogenase activity, wherein the cells are capable of producing n-propanol. Also described are methods of producing n-propanol comprising (a) cultivating the host cells having lactate dehydrogenase activity, lactaldehyde dehydrogenase activity, lactaldehyde reductase activity, propanediol dehydratase activity, and / or n-propanol dehydrogenase activity in a medium under suitable conditions to produce n-propanol; and (b) recovering the n-propanol. Methods of producing polypropylene from the recombinant n-propanol are also provided.

The invention discloses a solid substrate priming method of seeds of a seedless watermelon, relating to a seed treatment technology and comprising the following six steps of: firstly, selecting and pretreating a solid substrate, secondly, selecting and treating seeds, thirdly, priming the seeds, fourthly, separating the seeds from sawdust, fifthly, drying, and sixthly, storing the seeds at a low temperature. The method for conductivity measurement or activity measurement of dehydrogenase is adopted when the seeds are selected according to needs, the seeds with poor innate vigor are removed to avoid unnecessary priming waste. In the method, sawdust is used as the solid substrate, therefore, the material is easily available, the cost is low, the specific gravity and the friction coefficient are low, the operation is relatively convenient, the surface of a screen is less worn when in screening or separating, the seeds are cultured in darkness in an incubator so that electric power is saved, the priming effect of seeds is extremely ideal, and the positive effect on seed germination is superior to the priming effect of other solid substrates.

The present invention provides a process for highly efficiently producing ethanol at a high productivity consisting of using a coryneform bacterium which has been transformed by a DNA containing a gene expressing pyruvate decarboxylase activity and, if desired, a gene expressing alcohol dehydrogenase activity under a regulatory sequence allowing for the expression, under ethanol production conditions wherein this bacterium does not substantially proliferate to produce ethanol.

Provided are an amadoriase that is less likely to be influenced by oxygen concentration and a method and a reagent kit for measurement of HbA1c using such amadoriase. Provided are an amadoriase that is obtained via substitution of one or more amino acid residues at a position or positions corresponding to the position(s) selected from the group consisting of positions 280, 267, 269, 54, and 241 of the amadoriase derived from the genus Coniochaeta, a method for measurement of HbA1c, a reagent kit for measurement, and a sensor using such amadoriase. The modified amadoriase according to the invention has a lowered oxidase activity and an enhanced dehydrogenase activity, and this enables the use of an electron mediator, and this reduces the influence of oxygen concentration. Thus, HbA1c can be measured with high sensitivity.