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Petroleum hydrocarbon degrading mixed inoculum suitable for medium-temperature aerobic biological heap remediation system and applications of petroleum hydrocarbon degrading mixed inoculum

A technology of mixed bacterial agents and aerobic organisms, applied in the field of microbial remediation of organic contaminated soil, can solve the problems of limited biodegradation efficiency, achieve rapid and efficient repair process, increase porosity, and ensure metabolic activity

Pending Publication Date: 2018-04-10
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For petroleum hydrocarbon components composed of alkanes and aromatics, although they have the potential to be metabolized by organisms, their biodegradation efficiency is still limited due to the influence of metabolic conditions and substrate characteristics.

Method used

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  • Petroleum hydrocarbon degrading mixed inoculum suitable for medium-temperature aerobic biological heap remediation system and applications of petroleum hydrocarbon degrading mixed inoculum
  • Petroleum hydrocarbon degrading mixed inoculum suitable for medium-temperature aerobic biological heap remediation system and applications of petroleum hydrocarbon degrading mixed inoculum
  • Petroleum hydrocarbon degrading mixed inoculum suitable for medium-temperature aerobic biological heap remediation system and applications of petroleum hydrocarbon degrading mixed inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 petroleum hydrocarbon degradation mixed bacterial agent

[0026] The bacterial source sample used in this example comes from the long-term oil-contaminated soil around the oil wells in the Liaohe Oilfield. Weigh 3.0 g of the oil-contaminated soil, add 50 ml of inorganic salt culture medium, shake for 20 minutes, let stand for 5 minutes, take the supernatant and add To contain 0.3g·L -1 The alkane components in petroleum hydrocarbons and 0.2g·L -1 The aromatic components in petroleum hydrocarbons were enriched and screened in inorganic salt liquid medium for 7 days, and the cultures were transferred to 0.3g·L -1 Alkanes and 0.2g·L -1 Streak separation in aromatic hydrocarbon inorganic salt solid medium, select a single colony with good growth for three consecutive plate streak purification, the isolates were stored on beef extract peptone slant medium, and were identified to belong to Bacillus licheniformis and Bacillus licheniformis res...

Embodiment 2

[0032] Example 2 Application of petroleum hydrocarbon degradation mixed bacterial agent in remediation of petroleum-contaminated soil by mesophilic aerobic biopiles

[0033] Fully mix the prepared petroleum hydrocarbon degradation mixed bacteria agent with the petroleum-contaminated soil to be remediated, so that the number of bacteria and microorganisms in the remediation initial pile reaches 7.0×10 8 CFU·g -1 earth. Then build a biological pile with a width of 1-2m and a height of 1m ( figure 1 ). HDPE anti-seepage film is laid on the bottom of the pile, and a 10cm-thick gravel air-conducting layer is laid on the anti-seepage film. The gas injection pipeline and the air extraction pipeline are respectively laid in the air-conducting layer, and the air extraction pipeline is also provided with branches perpendicular to the horizontal plane. Suction line. The external end of the gas injection pipeline stack is connected to the pipeline heater, gas injection fan, atomizer a...

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Abstract

The invention belongs to the technical field of microbial remediation of organic polluted soil, and in particular relates to a petroleum hydrocarbon degrading mixed inoculum suitable for a medium-temperature aerobic biological heap remediation system and applications of the petroleum hydrocarbon degrading mixed inoculum. The mixed inoculum is prepared in a manner that bacillus licheniformis and pseudomonas fluorescens are mixed and immobilized, wherein bacillus licheniformis and pseudomonas fluorescens are mixed at the volume ratio of (3:1) to (5:1), a rice hull carrier after acidification andneutralizing of the pH is adopted for carrying out immobilization, and thus the mixed inoculum is prepared. When the total amount of bacterial microbes in the biological heap achieves 40-60 % of theinitial content, the mixed inoculum is supplied during the interval of stopping of forced ventilation of the heap, the total amount of bacteria in the heap is maintained to be 90-110 %, and meanwhile,the activity of dehydrogenase in the polluted soil is enabled to be 70-90 % of the initial value, so that the biological heap efficient remediation process of the petroleum-polluted soil is effectively realized.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of organic polluted soil, and in particular relates to a petroleum hydrocarbon degradation mixed bacterial agent suitable for a mesophilic aerobic bioreactor remediation system and its application. Background technique [0002] With the continuous development of the oil extraction industry, the continuous increase in the demand for extraction and the increasing difficulty of extraction, oil has become an increasingly valuable energy source. However, with the rapid development of the petroleum industry, the soil pollution caused by oil and gas development is also becoming more and more serious. Petroleum hydrocarbon pollutants damage the soil structure and ecological functions, endangering the health and safety of organisms and even human beings. Among them, the hydrocarbon components accounting for more than half of the petroleum pollutant content are the main components of soil poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/14B09C1/10C12R1/10C12R1/39
CPCC12N1/20B09C1/10C12N11/14
Inventor 郭书海王卅张凯李凤梅
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
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