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Increased ethanol production by bacterial cells

A technology of ethanol and acetic acid, applied in the direction of bacteria, methods based on microorganisms, methods using microorganisms, etc., can solve the problems of harmful cell growth, lower membrane potential, etc., and achieve the effect of mitigating global warming

Inactive Publication Date: 2011-01-19
BIOCONVERSION TECH INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Acetic acid is therefore regarded as an undesirable waste product, and it also acts as an uncoupler that lowers the membrane potential and is detrimental to cell growth

Method used

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  • Increased ethanol production by bacterial cells
  • Increased ethanol production by bacterial cells
  • Increased ethanol production by bacterial cells

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Embodiment Construction

[0030] The BCT25-H strain is a lactate dehydrogenase-deficient Bacillus strain derived from the LLD-R strain (constructed according to Example 3 of WO 2007 / 110606). Under partial aerobic culture conditions at 65°C and 200 rpm, in 10 ml of 2TY medium (tryptone 16 g, yeast extract 10 g, sodium chloride 5 g, distilled water to 1000 ml, with 20% w / v NaOH to adjust the pH value to 7.0) and 56 mM glucose and contain different concentrations of glycerol (54, 108 and 216 mM) in 30 ml Sterilin sterilized bottles.

[0031] Growth studies showed that addition of glycerol significantly increased ethanol production without much change in formic acid levels (see Table 1 and Figure 4 ). Although the addition of glycerol is expected to produce lesser amounts of acetate, the slightly higher acetate levels in this study may be due to the fact that the growth conditions were not strictly anaerobic and some acetate was already produced due to over-metabolism.

[0032] Table 1 - Summary of eth...

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Abstract

Fermentation processes for production of ethanol include supplying a thermophilic microorganism lacking lactate dehydrogenase activity with sugars under conditions in which they metabolise them predominantly by the pyruvate-formate lyase pathway. Importantly, the processes also include supplying sufficient glycerol to convert all of the sugars to ethanol. A further embodiment of the invention includes supplying additional glycerol sufficient to convert the exogenous acetate present in biomass hydrolysates into ethanol. Any type of fermentation system can be used for these processes, but a preferred embodiment includes continuous cultures at high temperatures in which ethanol is removed continuously by vacuum evaporation.

Description

technical field [0001] The present invention relates to enhanced ethanol production by thermophilic bacteria such as Bacillus from mixed sugars, such as those derived from biomass hydrolysis. More specifically, the present invention relates to the use of waste glycerol from biodiesel production as a co-feedstock to increase ethanol production in these processes. Background technique [0002] Shama, G and Hartley, B.S. (Phil. Trans. Roy. Soc. Lond. A321, 555-568, 1987) observed that, like the wild-type strain (LLD-R), thermophilic spores lacking lactate dehydrogenase activity Bacillus mutant (LLD-15 strain) can metabolize a wide range of sugars under optimal anaerobic growth conditions. However, unlike the wild-type strain that mainly produces lactate as the main product, this mutant metabolizes these sugars by producing 1 mole of acetate, 1 mole of ethanol, and 2 moles of formate per mole of glucose equivalent consumed via the pyruvate-formate lyase pathway ( figure 1 ) (S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06C12P7/08
CPCC12Y101/01027Y02E50/16C12P7/10C12N9/0006C12P7/065C12P7/08Y02E50/17Y02E50/10C12N1/20C12N1/36C12N15/01C12R2001/07C12N1/205
Inventor 纳姆达尔·巴格哈伊-亚兹迪穆罕默德·贾韦德布赖恩·哈特利
Owner BIOCONVERSION TECH INC
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