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Method for investigating TTC-dehydrogenase activity for drinking water treated biologic

A technology for biological treatment and activity determination, used in biological testing, preparation of samples for testing, measurement of color/spectral properties, etc.

Inactive Publication Date: 2005-03-23
HARBIN INST OF TECH
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  • Abstract
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AI Technical Summary

Problems solved by technology

The TTC-dehydrogenase activity method of the present invention solves the detection problem of biological activity under the conditions of detection scale and low substrate concentration

Method used

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Embodiment Construction

[0005] Assay method of the present invention comprises following operation steps:

[0006] a. Collection and preparation of samples: Take 10-20 g of samples with a sampler and put them in sterile plates. Weigh 10-15g of sample into a Erlenmeyer flask containing 50-100mL of sterile water, cover with a sterile tampon, tie it tightly and place it on an oscillator to vibrate for 60min at a frequency of 240 times / min. The resulting biofilm mixture It can be used as a sample for TTC-DHA activity determination;

[0007] b. Sample concentration: transfer the biological floc suspension obtained in step a by shaking and removing the film into a 100mL centrifuge tube, centrifuge at a speed of 4000r / min for 10min, after standing still, discard the supernatant, and dissolve the precipitate in 10mL Wash with distilled water into a 25mL colorimetric tube, then add 7.5mL Tris-HCl buffer solution (pH=8.4), 2.5mL 0.36% Na 2 SO 3 solution, 2.5mL TTC solution with a volume concentration of 0.4...

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Abstract

The invention discloses a TTC- dehydrogenase activity testing method of drinking water, it relates to a environment monitoring method, especially a monitoring method to test biology activity for drinking water treatment. Nowadays, the method of testing biology activity of sewage biology treatment, because the solvent is vaporized, it is easily to break the stopper and overflow, so it leads to unstable result, so the biochemical analysis technology is limited to use in actual application. The method includes the following operation steppes: a. sample collecting and preparing, b. sample concentration, c. sample culturing, d. finishing enzyme reaction, e. color imetric analysis. The invention solves the biology activity testing problem under testing measure and low matrix concentration conditions, its result is stable, and has very high application value, it is propitious to be popularized and applied.

Description

Technical field: [0001] The invention relates to an environment monitoring method, in particular to a method for measuring biological activity in drinking water biological treatment. Background technique: [0002] The essence of the biodegradation process of organic pollutants in water is an enzymatic reaction process. Organic matter is oxidized and decomposed under the catalysis of microbial enzymes. In terms of microbial physiological metabolism, material metabolism and energy metabolism are closely related. The key link of energy metabolism in cells is the hydrogen (H + / e) Generation of energy through the respiratory chain. Dehydrogenase is the backbone enzyme system of the respiratory chain, and its coenzyme port (NAD + -nicotinamide adenine dinucleotide) or coenzyme port (NADP + -Nicotinamide adenine dinucleotide phosphate) is an important hydrogen transfer body, they can oxidize the hydrogen (H + / e) Transmit oxygen as a lipid molecule in the respiratory chain. H...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/40G01N21/31G01N33/52
Inventor 马放韩健任南琪杨基先
Owner HARBIN INST OF TECH
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