42 results about "Glycerol-3-phosphate dehydrogenase" patented technology

Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and / or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

A new approach for increase of ethanol yield during alcoholic fermentation via decrease of biomass accumulation by using ATP degrading enzymes is described. The part of the Saccharomyces cerevisiae SSB1 gene coding for cytosolic ATPase domain of ribosome associated chaperon cloned into expression cassette under control of the glycerol-3-phosphate dehydrogenase gene (GPD1) promoter was introduced into the S. cerevisiae BY4742 strain. The recombinant strains were tested for their ability to grow and produce ethanol during glucose anaerobic and aerobic cultivations. Strains overexpressing ATPase domain of SSB1 possessed decreased concentration of intracellular ATP. They accumulated elevated amounts of ethanol and were characterized by decreased biomass accumulation as compared to the wild-type strain under both anaerobic and aerobic conditions. Similarly, the apyrase gene apy from E. coli encoding ATP / ADP hydrolyzing phosphatase and ATPase domain of SSB1 gene of S. cerevisiae were co-expressed under the control of galactose-inducible GAL1 promoter. The recombinant S. cerevisiae strains revealed slight reduction of biomass accumulation, while specific ATPase activity, ethanol accumulation and yield during alcoholic galactose fermentation under semi anaerobic conditions were increased.

The invention discloses methods for genetically transforming a plant so that it expresses a glycerol-3-phosphate dehydrogenase. The method raises levels glycerol-3-phosphate in comparison to the wild type, leading to increased stress tolerance, and altered fatty acid content in glycerolipids. The method also produces plants having improved phosphorus utilization.

The invention relates to building and application of engineered strains capable of producing xylitol and ethanol at a high temperature simultaneously with a high yield. Concretely, N376F mutant genes of saccharomyces cerevisiae galactose permease (hexose transporter protein of hexose for transporting galactose and glucose) (ScGAL2) are effectively expressed in heat-resistant yeast strains YZJ015 by a gene engineering transformation method; meanwhile, K.marxianus glycerol-3-phosphate dehydrogenase (KmGPD1) genes are removed to cut off a glycerol production branch, so that the obtained strains YZJ119 obtain good capability of efficiently co-utilizing and fermenting glucose and xylose to produce the ethanol and the xylitol at a high temperature (higher than 42 DEG C). Under the condition of 42 DEG C, the YZJ119 can simultaneously use 43.95g / l of xylose and 83.11g / l of glucose to produce 40.43g / l of xylitol and 34.66g / l of ethanol in 28 hours; the production speed of the xylitol is 1.44g / l / h; the production speed of the ethanol is 1.24g / l / h; the yield of the xylitol is 0.92g / g; and the yield of the ethanol is 0.42g / g.

Described are compounds capable of modulating (a) the biological activity of ADP-dependent glucokinase (ADPGK) and / or glycerol-3-phosphate dehydrogenase (GPD2) or (b) the expression of the gene encoding ADPGK or GPD2 for use in treating a disease (a) associated with aberrant cell proliferation, e.g., a neoplasm, or (b) of the immune system, e.g., an autoimmune disease.