35 results about "Glycerol 3-phosphate" patented technology

Glycerol-3-phosphate o-acyltransferase (GPAT) participates in the first step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids (PUFAs) produced in oils of oleaginous organisms. The present application provides a nucleic acid fragment isolated from Mortierella alpina encoding a GPAT that is suitable for use in the manufacture of oils enriched in omega fatty acids in oleaginous organisms. Most desirably, the substrate specificity of the instant GPAT will be particularly useful to enable accumulation of long-chain PUFAs having chain lengths equal to or greater than C20 in oleaginous yeast, such as Yarrowia lipolytica.

Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and / or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

The invention discloses methods for genetically transforming a plant so that it expresses a glycerol-3-phosphate dehydrogenase. The method raises levels glycerol-3-phosphate in comparison to the wild type, leading to increased stress tolerance, and altered fatty acid content in glycerolipids. The method also produces plants having improved phosphorus utilization.

Described are compounds capable of modulating (a) the biological activity of ADP-dependent glucokinase (ADPGK) and / or glycerol-3-phosphate dehydrogenase (GPD2) or (b) the expression of the gene encoding ADPGK or GPD2 for use in treating a disease (a) associated with aberrant cell proliferation, e.g., a neoplasm, or (b) of the immune system, e.g., an autoimmune disease.

The invention provides a vibrio anguillarum and edwardsiella tarda-resistant multi-valence live vaccine which is obtained by transferring a vibro anguillarum attenuated strain through a constructed recombinant expression vector, wherein a 3-phosphoglycerol dehydrogenase is displayed on the surface of a vibrio anguillarum attenuated strain cell by an ice nucleus protein outer membrane positioning element of the recombinant expression vector-expressed pseudomonas syringae and the fusion protein of the 3-phosphoglycerol dehydrogenase of the edwardsiella tarda through the ice nucleus protein outer membrane positioning element. The invention further provides a correlative expression vector and application. The vibrio anguillarum and edwardsiella tarda-resistant multi-valence live vaccine is unique and ingenious in design, vibrio anguillarum and edwardsiella tarda-resistant, good in immune protection effect, simple in use, safe, and low in cost. The invention provides a safe, efficient and ecumenical mode to the diseases for preventing the cultured fishes, has practical business development and application value, and is suitable for the large-scale popularization and application.

The invention has supplied a yeast germ for producing glycerine with high productivity and its preparing method and application. In the yeast germ, the Dunaliella salina glycerol phosphate dehydrogenize is introduced, so that the glycerine with good quality can be produced with high productivity and low cost.

The invention provides a Cem GPDH (Glycerol-3-phosphate dehydrogenase) gene and an application of the Cem GPDH gene in the aspect of increase of cellular fatty acid content. A nucleotide sequence of the Cem GPDH gene is represented as SEQ ID NO: 1. The gene is derived from Chlorella ellipsoidea, and GPDH is coded. G3P (Glycerol-3-phosphate) synthesized through catalysis by GPDH is an important raw material for synthesis of TAG (triacylglycerol), the enzyme participates in mitochondria G3P shuttle, provides electrons for a respiratory chain, is a key enzyme for G3P synthesis, is one of key enzymes for connecting glycometabolism and lipid metabolism and has an important effect on lipid synthesis and energy metabolism in plants; and besides, the gene can remarkably increase the total fatty acid content of cells when utilized to convert yeast cells, plant cells and microalgae cells.

The invention discloses a transgenic Chlamydomonas for improving fatty acid content of Chlamydomonas reinhardtii, as well as a construction method and application thereof. The construction method comprises the following steps: respectively constructing a lysophosphatidic acid acyltransferase gene recombination expression vector and a glycerol 3-phosphate acyl transferase gene recombination expression vector; and respectively or jointly converting the lysophosphatidic acid acyltransferase gene recombination expression vector and the glycerol 3-phosphate acyl transferase gene recombination expression vector to the Chlamydomonas reinhardtii cell, thereby obtaining converted lysophosphatidic acid acyltransferase gene and / or glycerol 3-phosphate acyl transferase gene Chlamydomonas reinhardtii. According to the method, exogenous genes are converted into the Chlamydomonas reinhardtii, thus, oil production of the Chlamydomonas reinhardtii is increased, compared with the physicochemical methods, such as mutagenesis, effects of the method provided by the invention are more remarkable, and the Chlamydomonas reinhardtii has genetic stability; the obtained transgenic Chlamydomonas has increased oil production and lowered polyunsaturated fatty acids proportion, and saturation and modification steps in a biodiesel production process are reduced.