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Inorganic phosphorus (phosphate radical) diagnostic and measuring kit and method for measuring inorganic phosphorus (phosphate radical) concentration

A phosphate root and inorganic phosphorus technology, which is applied in the field of inorganic phosphorus diagnosis/determination kits and the determination of inorganic phosphorus concentration, can solve the problem of high results

Inactive Publication Date: 2009-12-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, jaundice, hemolysis, and fat-turbid serum have light absorption at 340nm, and a blank specimen must be prepared, otherwise the result will be high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0046] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0047] Stabilizer 500mmol / L

[0048] Coenzyme 3mmol / L

[0049] Glutamine Synthetase 10000U / L

[0050] Glycerol kinase 12000U / L

[0051] Glycerol-3-phosphate dehydrogenase 12000U / L

[0052] Glutamine 12mmol / L

[0053] Adenosine diphosphate 4mmol / L

[0054] Glycerin 15mmol / L

[0055] Magnesium chloride 2mmol / L

[0056] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured inorganic phosphorus (phosphate) sample to the ...

Embodiment 2

[0060] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is double reagent, comprises:

[0061] Reagent 1

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] Coenzyme 3mmol / L

[0065] Glutamine 12mmol / L

[0066] Adenosine diphosphate 4mmol / L

[0067] Glycerin 15mmol / L

[0068] Magnesium chloride 2mmol / L

[0069] Reagent 2

[0070] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0071] Stabilizer 50mmol / L

[0072] Glutamine Synthetase 10000U / L

[0073] Glycerol kinase 12000U / L

[0074] Glycerol-3-phosphate dehydrogenase 12000U / L

[0075] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0076] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance ≤ 0.1, test main wavelength 340nm, test second...

Embodiment 3

[0079] The inorganic phosphorus (phosphate radical) diagnosis / measurement reagent of the present embodiment is three reagents, comprises:

[0080] Reagent 1

[0081] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0082] Stabilizer 50mmol / L

[0083] Coenzyme 3mmol / L

[0084] Glutamine 12mmol / L

[0085] Adenosine diphosphate 4mmol / L

[0086] Glycerin 15mmol / L

[0087] Magnesium chloride 2mmol / L

[0088] Reagent 2

[0089] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0090] Stabilizer 500mmol / L

[0091] Glycerol kinase 12000U / L

[0092] Glycerol-3-phosphate dehydrogenase 12000U / L

[0093] Reagent 3

[0094] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0095] Stabilizer 500mmol / L

[0096] Glutamine Synthetase 10000U / L

[0097] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0098] When measuring the conce...

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Abstract

The invention relates to an inorganic phosphorus (phosphate radical) diagnostic and measuring kit utilizing technologies of an enzymatic-colorimetric method and an enzyme-link method, also relates to a method and a principle of measuring inorganic phosphorus (phosphate radical) concentration and compositions and components of reagents, and belongs to the technical field of testing and measuring of medical science, food, and environment. The kit mainly comprises the following compositions: buffer solution, coenzyme, glutamine, adenosine diphosphate, glycerin, magnesium chloride, glutamine synthetase, glycerol kinase, glycerol-3-phosphate dehydrigebase and stabilizer; samples are mixed with the reagents in certain volume ratio to perform a series of enzymatic reactions; then reactants are placed under a UV / visible analyzer; and the ascending level of absorbance is tested at the position where dominant wave length is 340nm so as to measure and calculate the inorganic phosphorus (phosphate radical) concentration.

Description

technical field [0001] The invention relates to an inorganic phosphorus (phosphate) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring the concentration of the inorganic phosphorus (phosphate), which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] 87% of the phosphorus in the human body exists in the bones. The phosphorus in the blood and the phosphorus in the bones maintain a dynamic balance. There is a certain relationship between the concentration of calcium and phosphorus in the blood. When the blood calcium increases in normal people, the blood phosphorus decreases, and vice versa. Of course. The product of [calcium] and [phosphorus] concentrations in plasma remains within a certain range; When the product of the two is greater than 40, calcium and phosphorus are deposited in the bone tissue in the form of bone salt, and when the product is greater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N21/25G01N33/84C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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