95 results about "F1 hybrid" patented technology

The invention relates to a constructing method of hybridized megalobrama skolkovii culter with fast growth and few intermuscular bones. Distant hybridization is conducted between Qiantang River and Guangdong Dong River megalobrama terminalis as a female parent, and a novel and good combination of Changjiang River system culter alburnus being a male parent, distant hybridization is conducted, novel megalobrama skolkovii culter hybridization of the superior combination of Dong River megalobrama terminalis (female)*culter alburnus (male) F1 is screened out. With the four groups of self-bred progenies of megalobrama amblycephala (female)*culter alburnus (male) F1 hybrid offspring, megalobrama terminalis, culter alburnus and megalobrama amblycephala being a contrast group, differences like growth performance, shapes and intermuscular bone traits of MT (female)*CA (male) F1 hybrid offspring are compared and analyzed. Results show that 1, MT (female)*CA (male) represents remarkable hyper-parent growth advantages (P<0.05), and the total weight is also remarkably higher than MA (female)*CA (male); 2, Dong River megalobrama terminalis (female)* culter alburnus (male) F1 is the novel hybridized megalobrama skolkovii culter having the advantages that the intermuscular bones are reduced, deep processing of fish is promoted and the eating quality is improved.

The invention discloses an SSR primer set and method for seed purity identification of the hot pepper variety F1 hybrid-Huifeng No. 2. The SSR primer set comprises SSR molecular markers L0143 and L0622 and primer pairs corresponding to the SSR molecular markers. Any primer pair can be utilized for distinguishing the hot pepper variety F1 hybrid-Huifeng No. 2 from the female parent and the male parent of the hot pepper variety F1 hybrid-Huifeng No. 2, and can rapidly detect the seed purity of the hot pepper variety F1 hybrid-Huifeng No. 2. In addition, the primer set can also be used for seed authenticity identification of hot pepper varieties F1 hybrid-Yuehong No. 1, Capsicum annuum L.syn.C.frutescens L and F1 hybrid-Huifeng No. 5. The method has the advantages of being rapid, accurate, low in cost, easy to implement and the like, can replace a traditional hybrid seed purity identification method, and has the high commercial application value.

The invention relates to the field of breeding and specifically relates to a multi-ecological-area recurrent mixed selection - mixed planting cotton breeding method, which comprises the following steps of: 1) preparing cotton hybridized combinations; 2) reproducing generation F1 hybrid seeds in the south for increasing the generation; 3) respectively sowing generation F2 seeds in cotton regions of the Yellow River Valley, the Yangtze River Valley and the northwest inland; 4) selecting generation F3 seeds of excellent individual plants and reproducing in the south for increasing the generation; 5) respectively sowing generation F4 seeds in the cotton regions of the Yellow River Valley, the Yangtze River Valley and the northwest inland; and 6) selecting generation F6 seeds of excellent individual plants, sowing the seeds in hybrid seed production fields and selecting excellent individual plants according to breeding purposes. The method provided by the invention complements a modified backcrossing and recurrent selection method and improves the yield, the quality and the insect resistance. The method has the advantages of high breeding efficiency, diversified varieties, high adaptability, high purity and good consistency, and lays a foundation for breeding better varieties in future.

The present invention relates to a breeding method for a black spot resistant sweet potato variety, and belongs to the technical field of hybridization breeding. The method of the present invention comprises steps of: acquiring F1 hybrid true potato seeds, screening single plants of the black spot resistant sweet potatoes, and performing fast breeding. The assessment and selection of the black spot resistant material is performed at the first round so as to eliminate disease-resistless materials; and it is maximally ensured that the black spot resistant single plants in the hybrid cultivars are selected. Given that ''the number of the selected black spot resistant single plants'' is much smaller than ''the number of the single plants that are selected according to the yield level''; so that the number of the single plants that need to be assessed in the field decreases dramatically and the workload is significantly reduced; an experiment of comparison between yields of geographically different locations can be performed at a time and the breeding period can be foreshortened by 2 to 3 years. The breeding method provided by the invention has the characteristics of simple breeding process, short breeding cycle, high accuracy, less blindness and high efficiency. The novel black spot resistant sweet potato variety with good comprehensive properties is bred, and has good economic, social and ecological benefits.

The present invention relates to a new cotton (Gossypium hirsutum) variety, and more particularly to cotton plants and cotton seeds of this new variety. The invention also relates to F1 hybrid cotton plants and seeds produced using the new variety, as well as to transgenic cotton plants and seeds produced by transformation of this new variety.

The invention provides a high-yield high-quality fast breeding method of sasanqua. The method comprises the following steps of (1) screening breeding parents; (2) hybridizing the breeding parents; harvesting generation F1 hybrid seeds; (3) performing anther induction culture on the generation F1 hybrid seeds; (4) performing callus tissue differentiation culture; (5) performing strong seedling rooting culture; (6) performing domestication transplanting; (7) performing field fixed planting. The anther culture technology is combined with a conventional hybridizing breeding measure to be applied to breeding practice of the sasanqua; sasanqua flower culture regeneration plants are obtained for the first time; through further sasanqua economic index and unsaturated grease quality index screening, the high-yield high-quality sasanqua new strains are successfully obtained. Compared with a pure hybridizing breeding method with dozens of years of breeding period, the method provided by the invention has the advantages that the breeding years are greatly shortened; the breeding efficiency is improved; the sasanqua breeding process is greatly accelerated; the important significance is realizedon the sasanqua improved type breeding and popularization and health and fast development of sasanqua industry.

The invention discloses a salt-tolerant wheat breeding method for screening an excellent salt-tolerant strain by means of anther culture of haploid. The breeding method comprises the steps that 1, a salt-tolerant wheat resource and a breeding main parent are screened; 2, salt-tolerant wheat screening pressure is determined; 3, the salt-tolerant wheat resource is hybridized with the breeding main parent, and an F1 hybrid species is obtained; 4, the F1 hybrid species is subjected to anther culture, and a haploid plant is obtained; 5, subculture root strengthening of the haploid plant is conducted; 6, the haploid plant is subjected to root-soaking reduplication; 7, anther-cultured seedlings are subjected to single-plant seed collection, an anther culture family is obtained, screening of agronomic characteristics and salt resistance is conducted, and the novel salt-tolerant wheat strain is obtained. According to the salt-tolerant wheat breeding method for screening the excellent salt-tolerant strain by means of anther culture of the haploid, the salt-tolerant wheat breeding cycle is significantly shortened, the salt-tolerant wheat breeding speed is greatly increased, salt-tolerant wheat breeding can be achieved within three years, and important industrial value is achieved.

The invention discloses a method for culturing triploid petunia. The method comprises the following steps: selecting a diploid petunia combination with strong heterosis as a crossing parent, selecting proper flower buds in the flowering period of a male parent, carrying out chemical mutagenesis by adopting mixed liquid of colchicine, oryzalin and trifluralin to generate 2n pollens, pollinating the 2n pollens to a diploid female parent stigma to obtain F1 hybridized seeds, and hybridizing n pollens the male parent of which is not subjected to chemical mutagenesis with the female parent to obtain contrast diploid F1 hybrid seeds. The adult plants developed from the F1 hybridized seeds are compared with the contrast diploid F1 hybridized filial generations in the aspects of agronomic trait and anatomy, and on this basis, the number of chromosomes can be detected so as to finally determine the triploid plant. According to the method disclosed by the invention, the breeding period is short, the operation is convenient, the mutagenesis efficiency is high and the application prospect is good.

The invention discloses a method for delaying the hatching of F1 hybrids of incubated silkworm eggs in hatching. The F1 hybrids of silkworm eggs in hatching are subjected to three-section type low-temperature preservation, and graine embryos of the F1 hybrids of silkworm eggs of mulberry silkworms are grown through the hatching processing to a body pigmentation stage, and then are continuously preserved at the certain environment temperature; 10 silkworm eggs boxes are bundled by a plastic rope, and put in a double-layered black cloth bag wrung after being soaked in water, and the silkworm eggs in the 10 silkworm eggs boxes are uniformly shaken and horizontally placed; and the black cloth bag wrapping the silkworm eggs boxes is put in a certain temperature environment for preservation, and then is transferred in a refrigerator for refrigeration. Under the condition of not influencing the hatching rate and the growth of the F1 hybrids of hatched silkworm eggs, the gathering data of newly hatched silkworms is delayed by 1 to 25 days so as to be favorable for adjusting the production data of silkworm cocoons, and to reduce the influence of the natural disaster and the temperature anomaly on the growth of mulberry leaves and the influence of the silkworm poisoning and the silkworm disease and other disasters on the growth of the silkworm cocoons, and the method disclosed by the invention is a safe emergency disposal technology for sericulture, and has the hatching rate of more than 95%.

The invention relates to a breeding method of red glutinous rice, which comprises the following steps: (1) performing hybridization of black glutinous rice with species of red-seed husk non-glutinous rice to obtain single-cross F0, planting to obtain single-cross F1 hybrids; (2) performing hybridization by taking high-yield white glutinous rice species of Gui No. D1 as a female parent, and taking single-cross F1 hybrids obtained by hybridization of Yulin black glutinous rice with red-seed husk non-glutinous rice species of Huanghongzhan as a male parent to obtain multiple-cross F0; (3) planting the multiple-cross F0 to gain F1 which all have black seed husk; planting the F1 to gain F2, separating the F2 to obtain plants with black, red or white seed husk, selecting plant seeds with red seed husk and glutinous endosperm for planting; performing selfing for 6-8 generations to obtain the required red glutinous rice species. The rice bred by the method of the invention has good glutinous property, good growing vigour during field growing, strong stems, red coarse rice, and comparable yield to current high-yield white glutinous rice.

The invention discloses a rice seedling stage leaf albino trait gene Oscaacl and application thereof, and belongs to the field of rice breeding. A mutant gene Oscaacl is cloned from map positions in first and second leaf albino trait mutants of one seedling stage, and the genomic sequence of the gene is shown in SEQ ID NO.4; a wild type gene encodes one chloroplast envelope ATP / ADP transport protein. The Oscaacl gene is transferred into a rice maintainer line and a sterile line, and three-line and / or two-line sterile lines with seedling stage albino leaf traits are cultivated; when a hybridization generation F1 obtained through seed production is subjected to seedling stage authentication, and plants with albino leaves are sterile line selfing false hybrid. The gene Oscaacl has the application prospect in hybridization generation F1 hybrid purity identification and sterile line selfing reproduction breed conservation purity identification work of rice breeding, and is of great significance in improving seed purity and reducing hybrid rice production risk.

The invention discloses a molecular marker closely linked with rice blast Pi9 gene and an application thereof and belongs to the field of molecular marker-assisted breeding. The invention discloses primer pairs for detecting the marker closely linked with rice blast Pi9 gene and a method for detecting whether a rice variety or a plant line contains Pi9 gene or not by virtue of the primer pairs. The method comprises the following steps: (1) extracting genomic DNA of a to-be-detected rice sample; (2) carrying out PCR amplification through the primer pairs; (3) carrying out enzyme digestion on the PCR amplification product obtained in the step (2) with an endonuclease and carrying out electrophoresis; and (4) determining whether the to-be-detected rice sample contains the Pi9 gene or not according to the enzyme digestion results. By the method, whether the to-be-detected rice sample contains the Pi9 gene or not and the contained Pi9 gene is heterozygous or homozygous can be accurately determined and the purity of an F1 hybrid seed of one of the parents containing the Pi9 gene can also be identified and the method has application prospects in Pi9 gene-assisted breeding.

The invention belongs to the technical field of plant breeding, particularly relates to a method for improving the cabbage type rape by use of cabbage, and aims at providing a new option to the improvement of the cabbage type rape. The method for improving the cabbage type rape by use of cabbage comprises the following steps: (1) obtaining an interspecific hexaploid F1 hybrid; (2) obtaining and screening the hexaploid F1 hybrid and the backcross first-generation BC1F1 of cabbage type rape; (3) screening the BC1F2 generation. The method provided by the invention can be used for improving the cabbage type rape and is simple and easy to implement with short cycle.

The invention belongs to the technical field of biology, and relates to a method for quickly identifying the genetic purity of a glutinous corn hybrid. The method comprises the following steps of: extracting genomic deoxyribonucleic acid (DNA) from glutinous corn, performing polymerase chain reaction (PCR) amplification by using the screened sequence-related amplified polymorphism (SRAP) effective primer combination NAUSRem7 / NAUSRpm1 and random amplified polymorphic DNA (RAPD) effective primers NAUSR709 and NAUSR712, respectively performing non-denaturing polyacrylamide gel electrophoresis and agarose gel electrophoresis on a PCR product obtained through amplification, and shooting a DNA electrophoresis pattern; and comparing and analyzing the size and position difference of polymorphism amplified fragments formed due to the difference of DNA fragment sequences in the electrophoresis pattern, and identifying the genetic purity of the glutinous corn F1 hybrid, namely a Suyunuo 2 seed. The detection method has the advantages of marking stability, high accuracy, no influence of the growth stage and environment of a sample to be detected, low cost, capability of being performed in thewhole growth season, and the like.

The invention discloses a rice hybrid breeding material as well as a cultivation method and application thereof. A female parent material with a herbicide sensitive gene and a male parent material with the herbicide sensitive gene are obtained by specific promoter drive and antisense inhibit expression methods; the herbicide sensitive gene is just expressed in stamen; and the herbicide sensitive gene is just expressed in pistil; the female parent material and the male parent material are mixed in application; mechanized cropping is carried out; the female parent emasculation and male parent female removal are achieved by spraying the herbicide before heading; and the high-quality F1 hybrid seed is produced by one-way mating between parents. By adopting the method disclosed by the invention, a sterile line and a restorer line can be freely obtained; high-purity hybrid is obtained; mixing of the self-fruitfulness seed is effectively removed; especially, serious decline of hybrid purity caused by fertility recovery of the photo-thermo-sensitive sterile line under an adverse light temperature condition is avoided; the breeding process of the parents of the hybrid rice is simplified; manpower and material resources are saved; and the production efficiency is improved.

The invention discloses a method for preparing lifetime genic male-sterile rice and a method for cultivating hybrid rice by the lifetime genic male-sterile rice. In the method for preparing the lifetime genic male-sterile rice, the lifetime genic male-sterile rice is obtained by carrying out hybridization on a photo-thermo-sensitive genic male sterile line and a low temperature sensitive male sterile line. In the method for cultivating the hybrid rice, the hybrid rice is obtained by carrying out hybridization on the lifetime genic male-sterile rice and any conventional rice. The hybrid rice is prepared by using the lifetime genic male-sterile rice as a female parent and using the conventional rice as a male parent. F1 hybrids are normal and can be cultivated. The application of the lifetime genic male-sterile rice to the rice hybrids has the advantages that not only can the defect of failure of hybrid seed production, which is caused by instable fertilities of the two male sterile lines in a two-line method, be overcome, but also due to a large male parent selection range, compared with a three-line method, the method is easier to calculate the hybrid rice with high quality and high yield.

The invention discloses applications of a predominately gynoecious selfing line LC-03 in pumpkin breeding, particularly applications of cucurbita maxima LC-03 in pumpkin breeding. The accession number of the cucurbita maxima LC-03 is CGMCC NO.12032, and the cucurbita maxima LC-03 is also called the predominately gynoecious selfing line LC-03. The flowering periods of first female flowers of filial generations of the predominately gynoecious selfing line LC-03 that is adopted as a female parent and other selfing lines are earlier than those of male parents, female flower interval pitch numbers of the first female flowers are less than those of the male parents, and the filial generations shows early maturing, dense melons and melon growth potentials of the F1 hybrid. Transferred predominately gynoecious high-generation selfing lines show that the yield increasing effect is obvious, and therefore discovery and application of predominately gynoecious characters have increasingly functions for promoting development of the industry of pumpkin for seed use and pumpkin for table use and for increasing incomes of farmers. The popularization and application prospect of the hybridized combination of this type is wide.

The invention provides a culture method for rooting slash pine x Caribbean pine F1 hybrid needle fascicles in water. The culture method includes the following steps: the needle fascicles of a slash pine x Caribbean pine F1 hybrid seed tree are taken and disinfected with carbendazim solution for 2 to 3 hours; the bases of the needle fascicles are cut; the bases of the needle fascicles are immersed in NAA solution for 12 to 16 hours, and the needle fascicles are washed with clean water for 2 to 3 times and vertically placed in glass bottles loaded with nutrient solution; the needle fascicles are cultured in a plastic greenhouse which is shaded by a shading net, and the nutrient solution is replaced once each week. By researching the affection of the age of the seed tree of the slash pine x Caribbean pine F1 hybrid needle fascicles, the concentration of growth regulator, the ingredients of the nutrient solution and other factors on the rooting of the needle fascicles, the invention obtains the optimal culture method for rooting the needle fascicles in water, and the rooting rate of the needle fascicles reaches 91.96 percent. The material source is rich, the operating method is simple, and is convenient to use, and the rooting rate is high. The operating process cannot pollute the environment, and the culture method is safe to use, and can be used for rooting needle fascicles of pine trees in water.

The invention belongs to the fields of crop science and crop breeding, which precisely provides a method for cultivating a rapid selection new PTGMS line in wheat with anthers. The method comprises the following steps: 1) hybridizing a PTGMS line needing improving with a conventional wheat breed, with a PTGMS line needing improving as a maternal plant and a conventional wheat breed as a father plant, to acquire an F1 hybrid; 2) seeding the acquired F1 hybrids or the selected F3 seeds; 3) carrying out anther inoculation when the microspores of plant F1 or F3 develop to monocytes in a middle-advanced stage, to acquire a haploid; 4) carrying out chromosome doubling to the haploid acquired in step 3), to acquire a homozygous diploid; and; 5) acquiring the seed of the homozygous diploid and dividing the seed of the homozygous diploid into two equal shares, with one share being planted in an ecological environment to appraise the agronomic characters of the species and breed the seeds, with the other share being planted in a sterile environment to appraise the fertility of the species, thus selectively breeding the PTGMS line in wheat with the needed agronomic characters.

The invention discloses a breeding method of insect-resistant upland long stapled cotton. By adopting CRICAAS (Cotton Research Institute of Chinese Academy of Agricultural Sciences) No. 12 as a female parent and Darwinii cotton as a male parent, hybridization is carried out; F1 hybrid is used as a female parent, backcross on the F1 hybrid and the CRICAAS No. 12 is carried out for three times to obtain BC3F1; by using the BC3F1 as the female parent, hybridization on the BC3F1 and parental L41 of Xiangzamian No. 3 is carried out to obtain hybrid generation 1 used as a female parent, modification backcross on the hybrid generation 1 and the BC3F1 is carried out, and the backcross is carried out for four times to obtain BC4F1; then by using the BC4F1 as a female parent, the hybridization on the BC4F1 and Xinhai No. 5 is carried out, the backcross on the obtained hybrid generation 1 used as the female parent and the BC4F1 is carried out for four times; cotton-sealing inbreeding is carried out for six times, and separation is produced; finally, the insect-resistant long stapled cotton Xiang C176 is obtained. The yield of the Xiang C176 obtained by the invention is far higher than that of sea island cotton, and the insect-resistant upland long stapled cotton can be demonstrated and popularized in national main cotton planting areas, thereby increasing the self-supply ability of the long stapled cotton in China, and increasing the market competitiveness of high-grade raw cotton.

The invention relates to the technical field of biology, and in particular to a method for cultivating a japonica rice variety resistant to herbicide imazethapyr assisted by molecular marker. The method comprises the steps of screening an anti-imazethapyr rice material Jinjing 818 from 7403 parts of rice varieties resource materials, taking Jinjing 818 of japonica rice variety resistant to herbicide imazethapyr as a donor and conventional japonica rice variety as an acceptor for crossbreeding, obtaining F2 generation group through F1 hybrid selfing, using SSR molecular marker RM7413 to choose an individual corresponding to a linked molecular marker band of the donor Jinjing 818 herbicide resistance gene, namely homozygous genotype F2 generation strain and obtaining the stable japonica rice new variety resistant to the herbicide imazethapyr through F2 generation strain continuous selfing until obtain high-generation groups. The SSR molecular marker RM7413 and the molecular marker assisted cultivation method obtained by the invention will play an important role in genetic improvement of the japonica rice resistant to the herbicide imazethapyr.

The invention discloses a creation method of a pumpkin interspecific crossing recombinant inbred line. The method comprises the steps that winter squash is used a female parent, cucurbita moschata isused as a male parent, F1 hybrid seeds are obtained, then winter squash is used as a recurrent parent, and the interspecific crossing recombinant inbred line is obtained through a backcross and selfing method; the interspecific crossing recombinant inbred line has the performance of progeny reproduction. Along with increasement of the selfing generation number, the progeny fertility is gradually restored, the fertility of high-generation inbred lines of the fourth inbred generation and the fifth inbred generation is basically close to a normal level, and the reproductive performance of the interspecific crossing recombinant inbred line is good. By adopting the method, the problems can be solved that hybridization of cucurbita moschata and winter squash is difficult, and interspecific crossing hybrids and selfed progeny thereof are difficultly obtained.

The invention discloses a rice hybrid seed production method. The rice hybrid seed production method comprises the steps that firstly, a base sequence, a WA352 gene sequence and an exogenous reporter gene of a mitochondrial signal peptide are integrated into a wild rice chromosome, a transgenic sterile strain containing the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide is obtained, and the base sequence, the WA352 gene sequence and the exogenous reporter gene of the mitochondrial signal peptide are linked for expression; secondly, the transgenic sterile strain as a female parent is hybridized with a restorer line as a male parent, F1 hybrid seeds are obtained, the F1 hybrid seeds with the exogenous reporter gene are sterile seeds, and the remaining F1 hybrid seeds are normal hybrid rice seeds and are used for production. The method breaks through the confine of thought of the sterile gene and the restoring gene, male abortion is stable and thorough without affection of environmental conditions, the rate of outcrossing seed-setting is high, the seeds are an ideal material for recurrent selection, and more convenience is brought to the germplasm innovation process.

The invention discloses a dickeya zeae-resistant japonica rice breeding method. The breeding method comprises the following steps: (1) selecting a to-be-improved japonica rice material and a dickeya zeae resistant indica type rice; (2) identifying the crude toxin tolerance concentration of the selected to-be-improved japonica rice material; (3) hybridizing to obtain a triple-crossed F1 hybrid; (4) then performing anther culture in an indica rice eliminating culture medium on the triple-crossed F1 hybrid; (5) harvesting seeds of a single anther-cultured seedling to obtain an anther culture family and further performing agronomic trait and dickeya zeae resistance screening to obtain a new japonica rice line. According to the breeding method disclosed by the invention, a dickeya zeae-resistant genetic material in indica rice can be enabled to efficiently and exclusively permeate into the japonica rice, and dickeya zeae-resistant japonica rice breeding can be completed within about 3 years, so that the dickeya zeae-resistance breeding cycle is greatly shortened, and a breeding application value is greater.

The invention relates to a seedling identification method of the purity of palmatum leaf pigment gland double labeled hybrid cotton seeds, which comprises the following steps of: respectively sowing pigment gland labeled hybrid cotton F1 and male and female parent seeds thereof, and observing the presence and the amount of pigment glands on seedling necks and cotyledon, wherein pigment-glandless seeds are male parent seeds or seeds mixed with other pigment-glandless cotton, seeds with the pigment gland density between male parent seeds and female parent seeds are F1 hybrid seeds, and seeds with higher pigment gland density are female selfing parents or seeds mixed with other multi-pigment gland cotton; and determining the purity of F1 hybrid seeds according to the total number of measured cottonseeds and the quantity of seedlings with male parent, female parent and intermediate type pigment gland characters. The method provided by the invention is simple and easy to implement, fast and reliable, and solves the identification difficult problem of the purity of cotton hybrid seeds. The seed purity can be sampled for inspection at any time in the processes of acquisition, processing, storage, transportation and sale of hybrid cotton seeds according to needs, so that the production quality of the seeds is ensured, and the legitimate rights and interests of producers, sellers and users of the hybrid cotton seeds are protected.