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Cultivation method of rice germplasm with high-resistant starch and low-amylose starch

A high-resistant starch, low-amylose technology, used in gardening methods, botanical equipment and methods, gardening tools/equipment, etc.

Inactive Publication Date: 2013-06-12
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the fact that cultivating high-quality, high-yielding and high-RS rice materials will involve geographical distance, indica-japonica hybridization, and large segregation populations of offspring, and the anther regeneration technology of indica-japonica hybrid offspring is still highly dependent on genotype (including due to anther healing low induction rate of wound tissue, low differentiation rate and high albino seedlings), all of which are not suitable for the improvement of existing high RS rice materials

Method used

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  • Cultivation method of rice germplasm with high-resistant starch and low-amylose starch
  • Cultivation method of rice germplasm with high-resistant starch and low-amylose starch
  • Cultivation method of rice germplasm with high-resistant starch and low-amylose starch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Obtaining F1 of Zhaxima / Nanjing 46

[0032] In May 2011, 500 hybrid F1 seeds were obtained in Nanjing with Zhaxima as the female parent and Nanjing 46 as the male parent. Zhaxima is a local variety in Yunnan. The PS content of its rice was determined to be 8%, which is High RS characteristic germplasm resources; Nanjing 46 is a high-quality and high-yield japonica rice "Wuxiangjing 14" from Jiangsu Academy of Agricultural Sciences as the female parent, and it is crossed with Japanese high-quality japonica rice "Kanto 194", which is a mid-maturing late japonica rice type with high quality and high yield The japonica rice variety has an amylose content of 15% and a yield of 50g / plant.

Embodiment 2

[0033] Example 2 Design of each medium used for F1 of Zhaxima / Nanjing 46

[0034] a) Design of callus induction medium

[0035] The choice of basic medium:

[0036] Select the following components: white sugar 6%, agar strip 8g / L, additional 2,4-dichlorophenoxyacetic acid 1-3.0 mg / L, naphthalene acetic acid 0.5-3.0 mg / L, kinetin 0.2-0.3 mg / L , set two white granulated sugar concentrations of 30 g / L and 60 g / L, and take them respectively with M8, N6 and SK3’s macroelements, microelements, organic components and iron salt components as basic components to form six groups to form anthers Callus induction medium.

[0037] Each of the above-mentioned media was adjusted to pH 5.9 with sodium hydroxide and hydrochloric acid before autoclaving.

[0038] The F1 generation of hybrid rice (provided by Example 1, the same below)

[0039] experiment method:

[0040] In March 2012, the rice panicles at the mononuclear stage of the test were taken. After 1-10 days of low-temperature...

Embodiment 3

[0080] Example 3 Anther induced callus

[0081] Callus induction medium (provided by the best protocol of Example 2)

[0082] Take the single-nucleus flower spikes at the marginal stage of F1 hybrids, take the flag leaves and extract 0.5-1 cm flower spikes, put them in plastic bags after being sterilized with 75% ethanol, and take out the rice spikes after 3 days of low temperature pretreatment. The anther inoculation operations were all carried out under aseptic conditions on an ultra-clean workbench: the surface was first sterilized with 75% ethanol, and the outer sheath of the rice ears was removed and placed in a sterilized Erlenmeyer flask. Disinfect with 75% ethanol for 5 min, and then with 0.1% HgCl 2 Disinfect for 15 minutes and then rinse with sterile water for 3 times, cut off the husks of the sterilized rice ears, align the incisioned husks with the mouth of the triangular bottle with tweezers, and knock them into different anthers to heal the wounds. In the Erl...

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Abstract

The invention relates to a cultivation method of a rice germplasm with high-resistant starch and low-amylose starch. The cultivation method comprises the following steps of: hybridizing rice germplasm of screened high-resistant starch and good-quality high-yield rice germplasm to obtain an F1 hybrid; carrying out anther culture to obtain a high-frequency stable anther culturing strain; reduplicating the anther culturing strain, carrying out identification of resistant starch and amylase starch on the obtained seed and screening to obtain a high-yield rice germplasm with excellent character of the high-resistant starch and the low-amylose starch. The cultivating period of the method is only two years, the inductivity of a rice callus of the type is above 30%, the differentiation rate is 40%, and the average regeneration rate of anther cultivation is above 1.2%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a breeding method for cultivating rice with excellent eating quality and high resistant starch through the organic combination of conventional breeding, biotechnology breeding and physiological identification of functional components. technical background [0002] Resistant starch (RS) is also known as enzyme-resistant starch and digestible starch. Studies have shown that in the small intestine, RS cannot be digested and absorbed and provide glucose, but can be fermented by physiological bacteria in the large intestine to produce short-chain fatty acids and gas. RS is similar to dietary fiber in its metabolic properties. It can reduce the risk of some chronic diseases (such as diabetes, colorectal cancer and obesity) by reducing blood sugar levels after meals, controlling body weight, effectively preventing intestinal diseases and lowering blood lipids in diabetic patients , and als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00
Inventor 李霞田亲亲魏晓东方先文陆长梅
Owner JIANGSU ACAD OF AGRI SCI
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