Fast breeding method for black spot resistant sweet potato variety
A technology for anti-black spot and black spot disease, applied in the directions of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of long breeding years, heavy workload and high cost, and achieve simple breeding methods and the number of single plants. Reduced effects of short breeding cycles
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Embodiment 1
[0028] 1. Breeding process
[0029] (1) Hybrid combination configuration
[0030] Lushu 7, which is highly resistant to black spot and has high yield potential, was used as the female parent, and Jishu 15, which was resistant to black spot, was used as the male parent to sexually cross, and 3500 F1 hybrid seeds were obtained.
[0031] (2) Single plant screening of F 1 generation sweet potato
[0032] Sow the obtained 3,500 hybrid seeds in the vermiculite powder in the greenhouse, strengthen management after emergence, topping to promote branch growth, and select 3,000 healthy and healthy plants; each individual plant selects 4 growths that are basically the same, 4-5 Branches with internode length (about 15cm); 3 of them were subjected to inoculation induction of black spot conidia (Method A) (induction of black spot conidia refers to: implanting water-washed spores In sand, use a concentration of 1*10 4 individuals / ml black spot conidia suspension, watered and cultured at ...
Embodiment 2
[0046] 1. Breeding process
[0047] (1) Hybrid combination configuration
[0048] Ji 09184 with high resistance to black spot disease was used as the female parent, and Jishu 16 with good resistance to black spot disease was used as the male parent, and 1500 F1 hybrid seeds were obtained by sexual crossing.
[0049] (2) Single plant screening of F 1 generation sweet potato
[0050]Sow the obtained 1,500 hybrid seeds in the vermiculite powder in the greenhouse, strengthen management after emergence, topping to promote branch growth, and select 1,200 well-grown plants. Each individual plant selects 4 growths that are basically the same, 4-5 Branches with an internode length (about 15cm). Among them, 3 branches were implanted in fine sand for inoculation induction of black spot conidia (Method A). The highly susceptible variety Xushu 18 was used as a control. After 6 days, the disease grade was obtained by screening according to Table 1 of Example 1 and obtained a disease grade...
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