Rice seedling stage leaf albino trait gene Oscaacl and application thereof

Abstract

The invention discloses a rice seedling stage leaf albino trait gene Oscaacl and application thereof, and belongs to the field of rice breeding. A mutant gene Oscaacl is cloned from map positions in first and second leaf albino trait mutants of one seedling stage, and the genomic sequence of the gene is shown in SEQ ID NO.4; a wild type gene encodes one chloroplast envelope ATP / ADP transport protein. The Oscaacl gene is transferred into a rice maintainer line and a sterile line, and three-line and / or two-line sterile lines with seedling stage albino leaf traits are cultivated; when a hybridization generation F1 obtained through seed production is subjected to seedling stage authentication, and plants with albino leaves are sterile line selfing false hybrid. The gene Oscaacl has the application prospect in hybridization generation F1 hybrid purity identification and sterile line selfing reproduction breed conservation purity identification work of rice breeding, and is of great significance in improving seed purity and reducing hybrid rice production risk.

Description

technical field [0001] The invention belongs to the field of rice breeding, and in particular relates to the gene Oscaac1 for albinism of rice seedling leaves and its application. Background technique [0002] The chloroplast genome of higher plants generally encodes about 100 proteins, while the chloroplast contains at least more than 2000 proteins. Thus, the vast majority of chloroplast proteins are encoded by nuclear genes, translated in the cytoplasm and transported into the chloroplast (Abdallah et al., 2000). The transcription of plastid genes is accomplished by two types of RNA polymerases, plastid-encoded RNA polymerase (PEP) and nuclear gene-encoded RNA polymerase (NEP) (Maliga, 1988; Hajdukiewicz et al., 1997; Hedtke et al., 1997). These two RNA polymerases are responsible for the transcription of different types of chloroplast genes (Allison et al., 1996; Hajdukiewicz et al., 1997). For example, genes closely related to photosynthesis are transcribed by PEP, whi...

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Problems solved by technology

[0007] In the study of leaf color markers used in hybrid rice breeding, Dong Fenggao et al. (1994) introduced recessive light green leaf morphological markers into light- and temperature-sensitive male sterile lines through continuous backcrossing, in order to solve the problem caused by indica light. 1. It is difficult to guarantee the purity of hybrid rice seed production caused by the unstable fertility of thermosensitive genic male sterile lines

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