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Dickeya zeae-resistant japonica rice breeding method

A basal rot and bacterial technology, which is applied in the breeding field of japonica rice with bacterial basal rot resistance greatly shortened breeding cycle, can solve the problems of low screening efficiency, shortened breeding cycle and long breeding cycle.

Inactive Publication Date: 2017-03-15
WUXI NANLIGONG TECH DEV
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  • Abstract
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  • Claims
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Problems solved by technology

[0010] The purpose of the present invention is to address the shortcomings of long breeding cycle and low screening efficiency in the current breeding of bacterial root rot resistance japonica rice, and to provide a method that can efficiently and exclusively extract the genetic material of bacterial root rot resistance in indica rice Bacterial root rot resistance japonica rice breeding method that infiltrates japonica rice and greatly shortens the breeding cycle

Method used

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  • Dickeya zeae-resistant japonica rice breeding method

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preparation example Construction

[0039] (2.1) Preparation of crude toxin: Select the highly pathogenic toxin-producing strain Ech7 of bacterial root rot as the source of crude toxin, inoculate the strain in protein-free culture medium, and shake and culture at 30°C for 24 hours, then inoculate the bacterial culture at 4 Centrifuge at 10000×g for 15 min at ℃, and the obtained yellow supernatant is the crude toxin of rice bacterial root rot;

[0040] (2.2) Identify the crude toxin tolerance concentration of the selected japonica rice materials to be improved: respectively in the differentiation medium (the formula is: MS inorganic salt + MS organic matter + 3% sucrose + 0.6% plant gel + KT2.0mg / L + NAA0 .5mg / L+ hydrolyzed protein 1g / L, pH value 6.0), add a certain gradient concentration of crude toxin extract, and then transfer the anther callus induced by the japonica rice material selected in step (1) into the Differentiation medium, the callus differentiation rate was counted after 30 to 40 days of induction...

Embodiment

[0049] From 2010 to 2013, we selected the indica rice material Boyou 713 as the source of resistance to bacterial root rot, and the breeding main line J0118 as the japonica rice material to be improved, and adopted the technical route of the present invention to carry out breeding for bacterial root rot resistance. Three floriculture families with excellent agronomic traits and improved resistance to bacterial root rot were obtained. The specific operation procedures are as follows:

[0050] (1) Screening J0118 and Boyou 713 respectively as japonica rice materials to be improved and indica rice resistance sources to bacterial root rot:

[0051] Many years of breeding practice have found that the main breeding line J0118 has excellent agronomic traits, high yield, good rice quality, and early maturity, but is more susceptible to bacterial root rot. In the main season of 2010, the anthers of J0118 were inoculated on the indica rice medium for induction culture , it was found tha...

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Abstract

The invention discloses a dickeya zeae-resistant japonica rice breeding method. The breeding method comprises the following steps: (1) selecting a to-be-improved japonica rice material and a dickeya zeae resistant indica type rice; (2) identifying the crude toxin tolerance concentration of the selected to-be-improved japonica rice material; (3) hybridizing to obtain a triple-crossed F1 hybrid; (4) then performing anther culture in an indica rice eliminating culture medium on the triple-crossed F1 hybrid; (5) harvesting seeds of a single anther-cultured seedling to obtain an anther culture family and further performing agronomic trait and dickeya zeae resistance screening to obtain a new japonica rice line. According to the breeding method disclosed by the invention, a dickeya zeae-resistant genetic material in indica rice can be enabled to efficiently and exclusively permeate into the japonica rice, and dickeya zeae-resistant japonica rice breeding can be completed within about 3 years, so that the dickeya zeae-resistance breeding cycle is greatly shortened, and a breeding application value is greater.

Description

technical field [0001] The invention belongs to the field of agricultural high-tech, and relates to a method for breeding japonica rice with resistance to bacterial root rot, in particular to a method capable of efficiently and exclusively infiltrating genetic material resistant to bacterial root rot in indica rice into japonica rice 1. A breeding method that greatly shortens the breeding cycle of bacterial root rot-resistant japonica rice. Background technique [0002] Rice bacterial root rot ( Dickeya zeae ) is one of the more serious bacterial diseases on rice. After the disease was first reported in Japan in 1979, it has occurred in some Southeast Asian countries such as China, the Philippines, India, Indonesia, Bangladesh and North Korea. It has now become a disease in India. One of the important bacterial diseases of rice. In the early 1960s in my country, rice bacterial base rot caused serious damage in Fuding County, Fujian Province. Because it was a new disease,...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/02
Inventor 不公告发明人
Owner WUXI NANLIGONG TECH DEV
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