The invention discloses a preparation method of a mixed ferment. The preparation method comprises following steps: fresh highly-matured disease-insect-free Nanking cherry and plum-leaf crab are washed, are dried in the air, are subjected to denucleation, and are mixed and crushed at a weight ratio of 3:2; Kiwi berry is subjected to peeling, and individual crushing and beating; an obtained Nankingcherry and plum-leaf crab mixed solution and an obtained Kiwi berry pulp are introduced into two fermentation tanks respectively, 0.5 to 5wt% of honey is added, two obtained products are inoculated with Saccharomyces cerevisiae, and primary fermentation is carried out, two obtained fermentation products are inoculated with plant lactobacillus and lactobacillus acidophilus for secondary fermentation, and two obtained fermented broth are mixed, and 3 to 12 months of aging is carried out; filtering, pasteurization, and filling are carried out so as to obtain the mixed ferment finished product. According to the preparation method, the processing characteristics and the self local flavor characteristics of the three fruits are taken as base, the processing method is scientific and reasonable, release of effective components of the three fruits is promoted, the utilization rate of fruits poor in appearance and storage resistance is increased as far as possible, orchard worker income is increased, the obtained product is abundant in nutrients, moderate in sweet and sour taste, possesses fruit wine flavor, is abundant in mouthfeel levels, is rich in trace elements needed by human body andplant biologically active components (such as rutin, flavones, and protease) beneficial for human body, and possesses certain health care effect.