67 results about "Differentiation Inhibitor" patented technology

The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.

The invention belongs to the technical fields of immunology and tumor therapy, and relates to a preparation method and a use of a TCR gene modified CD8+T memory stem cell. The method comprises the following steps: co-incubating a tumor antigen and an immature dendritic cell to obtain a specific tumor antigen-supported mature dendritic cell, co-culturing the specific tumor antigen-supported maturedendritic cell and a CD8+ naive T cell, and adding a stem cell differentiation inhibitor to promote the generation of a CD8+ stem cell-like memory T lymphocyte; separating the CD8+ stem cell-like memory T lymphocyte; and cloning a T cell receptor gene (TCR) for specifically identifying a specific antigenic epitope, and carrying out lentivirus packaging or retroviral vector cotransfection on an autologous CD8+ Tscm cell to in-vitro prepare a CD8+ TCR-Tscm cell specific for different tumor specific antigens. The CD8+ TCR-Tscm cell prepared by the method has the advantages of overcoming of the tumor heterogeneity, high specificity, few adverse reactions, and highly-efficient and lasting tumor preventing and treating effects.

The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with a matrix of recombinant laminin-521 (laminin 11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.

The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.

The invention relates to Id knockout mammals having a disruption in at least one and at most three alleles of inhibitor of differentiation genes, Id1 and Id3. This results in reduction or prevention of a cell proliferative disorder in the mammal as compared to a wild-type mammal. In particular, tumor growth and/or metastasis is shown to be inhibited. Further, tumor growth is shown to have poor vascularization and extensive necrosis in Id knockout mammals lacking 3 out of 4 of the Id1, Id3 alleles (Id1−/−Id3±). Drug screening methods to select agents useful to affect activity or expression of Id1 or Id3 gene products are disclosed. Therapeutic methods employing selected agents in subjects in need of treatment and diagnostic methods and test kits to identify subjects having, or at risk of having, a neurological or cell proliferative disorder are also described.

There are provided a protein which comprises the amino acid sequence of an extracellular region of Ror2 and is water-soluble, an osteoclast differentiation inhibitor for inhibiting differentiation of a precursor cell of osteoclast into an osteoclast, which comprises the protein as described above, an inflammatory bone resorption therapeutic agent for treating inflammatory bone resorption, which comprises the protein as described above, a gene which codes a protein comprising the amino acid sequence of an extracellular region of Ror2 and being water-soluble, a recombinant vector which comprises the gene as described above, a method of manufacturing a protein, which comprises a step of causing expression of a gene coding a protein comprising the amino acid sequence of an extracellular region of Ror2 and being water-soluble in a microorganism to synthesize the protein and a step of extracting the protein from the microorganism into water or an aqueous solution, a method of inhibiting osteoclast differentiation, which uses the protein as described above, and a method of treating inflammatory bone resorption, which uses a protein as described above.

Provided is a method for suppressing an adipose differentiation comprising administering to a subject in need thereof a PPAR-v neddylation inhibitor for inhibiting adipocyte differentiation and a method for screening an adipocyte differentiation inhibitor using PPAR-v neddylation associated with adipocyte differentiation. The inhibitor of a neddylation pathway of PPAR-v inhibits the differentiation from mesenchymal stem cells to mast cells, and thus can be effectively used to treat obesity, and particularly, can be also useful in the treatment of severe obesity which cannot be excepted to be treated by existing obesity treatment agents.

The invention discloses a betulinic acid-amino acid derivative represented by a formula (I) and a preparation method thereof. The preparation method comprises the following steps of carrying out acylation reaction on a compound represented by a formula (a), acetic anhydride and pyridine under the protection of nitrogen so as to obtain a compound represented by a formula (b), reacting the compound of the formula (b) with oxalyl chloride so as to obtain a compound represented by a formula (c), then, respectively reacting the compound of the formula (c) with glycino methyl ester, L-alanine methyl ester, L-valine methyl ester, L-isoleucine methyl ester and L-glutamic acid methyl ester so as to obtain a compound represented by a formula (d), and washing, drying and purifying after reacting the compound of the formula (d) with LiOH so as to obtain the betulinic acid-amino acid derivative represented by the formula (I). The preparation method is high in synthetic efficiency and simple and convenient in process. The invention further discloses application of the betulinic acid-amino acid derivative represented by the formula (I) in preparation of a drug for treating osteoporosis; and the betulinic acid-amino acid derivative can be used as an osteoclast precursor differentiation inhibitor and has the obviously increased osteoclast precursor differentiation activity inhibition effect.

The invention discloses application of a Flot2 inhibitor to inhibition of osteoclast formation and/or osteoclastic activity for the first time. The osteoclast generation and bone resorption induced byRANKL can be obviously reduced after the Flot2 expression is inhibited by Flot2-siRNA specific interference. A study team of the inventor discovers that the Flot2 directly participates in the osteoclast generation and bone resorption functions, and is possible to become a novel target of an osteoclast differentiation inhibitor. On the basis of the inhibition effect of Flot2-siRNA on osteoclasts and the application prospects of RNA interference medicine, the application of the Flot2 inhibitor as a medicine of inhibiting the osteoclast generation is provided.