The invention provides a preparation method of a dynamic photonic crystal pattern for controlling a film refractive index in a wetting manner. The preparation method comprises the following steps: firstly preparing a two-dimensional photonic crystal on a transparent base material; then uniformly mixing acrylic monomers, a light sensitive agent and a cross-linking agent, obtaining a mixed solution,enabling the two-dimensional photonic crystal to contact a mixed solution, covering the transparent base material with a mask plate with patterns, curing under the ultraviolet rays, stripping a film,and obtaining a colloid photon crystal composite film which is controlled to be patterned in the wetting manner; and placing the composite film in a responsive solvent, or spraying the responsive solvent onto the composite film, and obtaining the dynamic photon crystal pattern. The polymerization degree of the reaction monomers is controlled by virtue of the mask plate, so that the wetting performance of different areas can be controlled, the refractive index of different areas can be controlled, a high bragg diffraction light appears in the film sheltered area, and the pattern is displayed in the area. The method is simple in process, the reaction condition is easy to control, and the patterning response is rapid.