54 results about "Cmv cytomegalovirus" patented technology

The invention discloses a HCMVPP65 antigenemia indirect immune fluorescence method detection kit, which uses cytomegalovirus-AD169 virus strain pp65 protein as the immunogen to immune a Balb/c mouse. The spleen cells of the immunized mouse and the myeloma cells of the mouse which belongs to the same type with the immune mouse are conventionally integrated, by indirect ELISA screening and finite dilution cloning, the hybridoma cell lines of the mouse cytomegalovirus pp65 protein cloning antibody are obtained, and the characteristics of the hybridoma cell lines are identified by ELISA, immune fluorescence experiment and other methods; two monoclonal antibodies that stably secrete the pp65 protein are established successfully and named respectively as 1A6 and 4A8. A monoclonal antibody which differs from the former report and aims at the pp65 protein of the cytomegalovirus (CMV) is prepared and a method used for preparing erythrocyte fast pyrolysis is established; compared with other detection kits which belongs to the same kind, the detection kit of the invention is faster, simpler and more convenient and has higher specificity and sensitivity.

A DNA vector comprises a first DNA sequence which is complementary to at least part of an alphavirus RNA genome and having the complement of complete alphavirus DNA genome replication regions, and a second DNA sequence encoding a paramyxovirus protein, particularly a respiratory syncytial virus fusion (RSV F) protein or a RSV F protein fragment that generates antibodies that specifically react with RSV F protein, the first and second DNA sequences being under the transcriptional control of a promoter, preferably a cytomegalovirus promoter, which may include Intron A. Such vectors also contain a further nucleotide sequence located between the promoter sequence and the alphavirus sequence to enhance the immunoprotective ability of the RSV F protein when expressed in vivo. Such DNA vectors may be used to immunize a host against disease caused by infection with RSV or other paramyxovirus, including a human host, by administration thereto, and may be formulated as immunogenic compositions with pharmaceutically-acceptable carriers for such purposes. Such vectors also may be used to produce antibodies for detection of RSV or other paramyxovirus infection in a sample.

The present invention relates to recombinant HCMV (human cytomegalovirus) expressing a pp65 polypeptide or fragment thereof fused to a heterologous or non-native polypeptide, in particular immunogenic and/or antigenic polypeptides. In particular, the heterologous gene products include antigenic or immunogenic polypeptides from a variety of pathogens, cellular genes, tumor antigens, and viruses. The recombinant viruses may advantageously be used in vaccine formulations including vaccines against a broad range of pathogens and antigens.

The present invention discloses one kind of gene recombinant human cytomegalovirus fusion protein pp150/MDBP and its preparation process and application, and relates to gene engineering technology, preventing vaccine, diagnosis reagent and other technology. The recombinant fusion protein pp150/MDBP is fusion protein formed through connecting serially the 197th amino acid in the 495-691 amino acid segment of human cytomegalovirus pp150 and the 64th amino acid in the 538-601 amino acid segment of MDBP protein. The 197th amino acid pp150 in the N-terminal of the fusion protein and the 64th amino acid of MDBP protein in the C-terminal of the fusion protein are connected through two amino acids including one Leu and one Glu, and the fusion protein has one increased Met and has whole length of 264 amino acids. The fusion protein is used in detecting human cytomegalovirus antibody and antigen, preparing antibody and protein chip, vaccine, ELISA detecting kit and other products.

The invention belongs to the technical field of biology and in particular discloses a recombinant herpesvirus of turkey (HVT). The recombinant HVT is capable of simultaneously expressing NDV and IBDVprotective antigen genes, and is a bacterial artificial chromosome (BAC) based on the HVT. The construction method comprises the following steps: inserting a VP2 gene expression cassette of an infectious bursal disease virus LX strain under regulation of a cytomegalovirus promoter CMV into a UL45-46 non-essential region of the HVT genome by utilizing a gene recombination technology; and insertingan F gene expression cassette of a newcastle diseases virus PX02/3 strain under regulation of a chicken beta-actin promoter pec into another non-essential region US1-10 of the HVT genome. The recombinant herpesvirus of turkey is a recombinant triplet poultry vaccine candidate strain capable of controlling Marek's disease of chickens and effectively resisting Newcastle disease and infectious bursaldisease.

The invention discloses a human cytomegalovirus (HCMV) anti-UL148 polyclonal antibody and a preparation method and application thereof. The HCMV anti-UL148 polyclonal antibody is obtained by taking UL148 recombinant fusion protein as an antigen to perform immunization on a rabbit to obtain serum and then performing purification treatment on the serum. The prepared polyclonal antibody has high purity and strong specificity; the UL148 recombinant protein is used for detecting the specificity of the polyclonal antibody; the Western blot result shows that for the HFF (Human Foreskin Fibroblast) infected with the HCMV, expression of UL148 genes is detected through the Western blot; and the polyclonal antibody can specifically recognize UL148 recombinant protein of which the size is about 54.5kDa. The polyclonal antibody disclosed by the invention can be used for development of HCMV diagnostic kits and HCMV related protein detection kits.

Disclosed are methods for gene therapy by administration of genetically modified viral vectors. Gene therapy vectors can include a cytomegalovirus vector encoding one or more therapeutic donor genes such as human telomerase reverse transcriptase (hTERT). These vectors can be used in exemplary gene therapy methods for maintaining or improving one or more aspects of a recipient's physiological wellness and/or longevity. The recombinant viral vector can be administered or received intranasally or as an injectable therapeutic

Provided are detection and assay of CMV that can rapidly amplify and detect the beta 2.7 gene originating from CMV at a certain temperature through a one-step operation. The mRNA of beta 2.7 gene originating from CMV is amplified by utilizing the combination of specifically amplifiable oligonucleotide primers and the RNA amplification step comprising these combinations of oligonucleotide primers is measured and detected by using an oligonucleotide probe that is marked with an intercalated fluorescent dye, to settle above topic.

The present invention relates to the field of virology. More specifically, the present invention provides methods and compositions useful for prevention and treatment of human cytomegalovirus (CMV). In one embodiment, a pharmaceutical composition comprises (a) emetine or a derivative thereof; (b) a human cytomegalovirus (HCMV) drug; and (c) a pharmaceutically acceptable carrier. In certain embodiments, the pharmaceutical composition further comprises an adjuvant. In a specific embodiment, the HCMV drug is ganciclovir. In such embodiments, emetine is present at about 1/10 to about 1/100 the normal dosage for amebiasis.

The invention discloses an LAMP (loop-mediated isothermal amplification) primer combination for detecting four types of eye infected viruses and an application. The primer combination comprises 24 single-stranded DNA molecules shown in sequences from 1 to 24. The invention also discloses the application of the primer combination, and further provides a method for identifying herpes simplex virus type I, herpes simplex virus type II, varicella-zoster viruses or cytomegalo viruses, a method for identifying whether the to-be-detected viruses are the herpes simplex virus type I, the herpes simplex virus type II, the varicella-zoster viruses or the cytomegalo viruses, and a method for identifying whether a to-be-detected sample is infected with the herpes simplex virus type I and/or the herpes simplex virus type II and/or the varicella-zoster viruses and/or the cytomegalo viruses. With the application of LAMP primers and the method, the herpes simplex virus type I, the herpes simplex virus type II, the varicella-zoster viruses and the cytomegalo viruses can be detected rapidly and accurately.

The invention discloses a primer group, a detection method and a kit for detecting drug resistance of cytomegalovirus. The primer group can highly specifically amplify a target fragment of a cytomegalovirus UL97 gene, can be used for analyzing drug resistance gene site mutation, and provides theoretical guidance for clinical use. The detection method provided by the invention is simple and accurate to operate and high in specificity.