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Mammalian cells expressing cytomegalovirus antigens

A cytomegalovirus, cellular technology applied in the field of mammalian cells expressing cytomegalovirus antigens

Active Publication Date: 2017-08-18
GLAXOSMITHKLINE BIOLOGICALS SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, recombinant production of CMV pentameric complexes remains challenging

Method used

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  • Mammalian cells expressing cytomegalovirus antigens
  • Mammalian cells expressing cytomegalovirus antigens
  • Mammalian cells expressing cytomegalovirus antigens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0184] This example relates to the production of CMV pentameric protein complexes by a CHO cell line in which the coding sequence for the pentameric subunit is stably integrated into the chromosome. Said cell lines are also referred to as CHO stable cell lines.

[0185] As shown in this example, these stable CHO cell lines produce functional CMV pentamers in which all five subunits assemble in a native conformation. The yield is high, enabling the generation of pentameric complexes on a large commercial scale. These cell lines are particularly suitable for the large-scale manufacture of CMV vaccines using pentamers.

[0186] As shown in this example, the nucleotide sequences encoding the extracellular domain of gH, gL, pUL128, pUL130, and pUL131 were cloned into single or double expression vectors with a subgenomic promoter and an IRES that Subunits and IRES drive the expression of individual components ( figure 1 ). Specifically, the coding sequences for the gH extracellu...

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PUM

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Abstract

This invention relates to cytomegalovirus (CMV) proteins suitable for vaccine uses. Provided herein are mammalian host cells, in particular CHO cells, in which the sequence(s) encoding CMV proteins gH, gL, pUL128, pUL130, pUL131 (or a complex-forming fragment thereof) are stably integrated into the genome.

Description

[0001] field of invention [0002] The present invention relates to host cells expressing cytomegalovirus (CMV) proteins suitable for vaccine use. [0003] Background of the invention [0004] Cytomegalovirus is a genus of viruses belonging to the family of viruses known as Herpesviridae or Herpesviruses. The species that infects humans is commonly referred to as human cytomegalovirus (HCMV) or human herpesvirus-5 (HHV-5). Within the Herpesviridae family, HCMV belongs to the subfamily Bherpesviridae, which also includes cytomegaloviruses from other mammals. [0005] Although they can be found throughout the body, HCMV infections are often associated with the salivary glands. HCMV infects 50% to 80% of adults in the United States (40% worldwide), as indicated by the presence of antibodies in large numbers of the general population. HCMV infection is usually unnoticed in healthy individuals but can be fatal in immunocompromised persons such as HIV-infected persons, organ trans...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/10C07K14/045
CPCC07K14/005C12N2510/02C12N2710/16122C12N2710/16151C12N2710/16134A61K39/00A61P31/12C12N5/0682
Inventor A.卡菲C.齐费里I.霍夫曼H.劳克斯A.利尔雅Y.温
Owner GLAXOSMITHKLINE BIOLOGICALS SA
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