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Gene recombinant human cytomegalovirus fusion protein pp150/MDBP, preparation process and application thereof

A human cytomegalovirus and fusion protein technology, applied in DNA preparation, recombinant DNA technology, peptide/protein components, etc., can solve problems such as lethality and active infection, increase expression and yield, and reduce production costs Effect

Inactive Publication Date: 2006-04-26
SHANDONG MEDICAL BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After normal people are infected with HCMV, they do not show obvious clinical symptoms, but for people with low body immunity, the recurrence of primary infection and virus latent infection can cause active infection, and can cause severe or even fatal disease

Method used

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  • Gene recombinant human cytomegalovirus fusion protein pp150/MDBP, preparation process and application thereof
  • Gene recombinant human cytomegalovirus fusion protein pp150/MDBP, preparation process and application thereof
  • Gene recombinant human cytomegalovirus fusion protein pp150/MDBP, preparation process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0176] Below in conjunction with embodiment the content of invention is further elaborated:

[0177] 1. Amplification of fusion protein expression genes

[0178] 1.1 Cloning of human cytomegalovirus pp150 gene UL32 gene fragment

[0179] The DNA sequence of the screened pp150 protein epitope is:

[0180] CTC GTC TCC CCG CAG GTG ACC AAG GCC AGC CCG GGA AGG GTC CGT CGG GAC AGC GCG TGG

[0181] GAC GTG AGG CCG CTC ACG GAG ACC AGA GGG GAT CTT TTC TCG GGC GAC GAG GAT TCC GAC

[0182] AGC TCG GAT GGC TAT CCC CCC AAC CGT CAA GAT CCG CGT TTC ACC GAC ACG CTG GTG GAC

[0183] ATC ACG GAT ACC GAG ACG AGC GCC AAA CCG CCC GTC ACC ACC GCG TAC AAG TTC GAG CAA

[0184] CCG ACG TTG ACG TTC GGC GCC GGA GTT AAC GTT CCT GCT GGC GCC GGC GCT GCC ATC CTC

[0185] ACG CCG ACG CCT GTC AAT CCT TCC ACG GCC CCC GCT CCG GCC CCG ACA CCT ACC TTC GCG

[0186] GGT ACC CAA ACC CCG GTC AAC GGT AAC TCG CCC TGG GCT CCG ACG GCG CCG TTG CCC GGG

[0187] GAT TAG AAC CCC GCC AAC TGG CCG CGC GAA CGC GCG TGG GCC...

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PUM

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Abstract

The present invention discloses one kind of gene recombinant human cytomegalovirus fusion protein pp150 / MDBP and its preparation process and application, and relates to gene engineering technology, preventing vaccine, diagnosis reagent and other technology. The recombinant fusion protein pp150 / MDBP is fusion protein formed through connecting serially the 197th amino acid in the 495-691 amino acid segment of human cytomegalovirus pp150 and the 64th amino acid in the 538-601 amino acid segment of MDBP protein. The 197th amino acid pp150 in the N-terminal of the fusion protein and the 64th amino acid of MDBP protein in the C-terminal of the fusion protein are connected through two amino acids including one Leu and one Glu, and the fusion protein has one increased Met and has whole length of 264 amino acids. The fusion protein is used in detecting human cytomegalovirus antibody and antigen, preparing antibody and protein chip, vaccine, ELISA detecting kit and other products.

Description

technical field [0001] The present invention relates to a gene recombinant human cytomegalovirus fusion protein, in particular to a gene recombinant human cytomegalovirus fusion protein pp150 / MDBP and its preparation method and application, belonging to the technical fields of genetic engineering technology, preventive vaccines and diagnostic reagents . Background technique [0002] Human cytomegalovirus (HCMV) infection is a common clinical infectious disease that seriously endangers human health. It can spread through various ways. After normal people are infected with HCMV, they do not show obvious clinical symptoms, but for people with low body immunity, the recurrence of primary infection and virus latent infection can cause active infection, and can cause severe or even fatal disease. HMCV infection is an important cause of high morbidity and mortality in organ transplants, bone marrow transplants, immunocompromised patients, and patients on immunosuppressive therap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/62A61K38/17C07K19/00C12N15/09C12N15/10G01N33/53
Inventor 高雪芹郭大东韩金祥
Owner SHANDONG MEDICAL BIO TECH RES CENT
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