48 results about "Avian orthoreovirus" patented technology

An isolated polypeptide comprising an amino acid sequence corresponding to the amino acid residues forming a full or partial alpha-helical domain, the hinge domain, the beta-triple spiral domain and afull or partial globular head domain of an avian reovirus sigma C protein, and lacking the amino acid sequence that is N-terminal to said alpha-helical domain is provided. Furthermore, a vaccine comprising, or a viral vector expressing, at least one of the isolated polypeptides of the present invention is provided.

The present invention relates to novel strains of avian reovirus isolated from severe cases of Runting Stunting Syndrome in young broiler chickens in southeast United States. The invention is directed to avian reoviruses that impair digestion in poultry, diagnostic assays using nucleotide- or amino acid-specific components of such viruses, and to vaccines that protect chickens from disease caused by such viruses. Nucleotide sequences for the S1 gene, encoding the sigma C minor outer capsid protein, were amplified, and the nucleotide and predicted amino acid sequences were compared with sequences from other recently isolated reovirus field isolates and vaccine strains. Antigenic and molecular characterization of the newly isolated reoviruses revealed a lack of homogeneity with current U.S. isolates, with less than 60% percent amino acid similarity across the sigma C protein. Sequence comparisons with previously reported malabsorption isolates from Europe and Asia revealed a higher amino acid similarity, approaching 80%.

This invention relates to novel unique strains avian reoviridae that were isolated from severe cases of Runting Stunting Syndrome. The present invention also relates to the isolation and uses of novel unique avian reoviridae, diagnostic assays using nucleotide or amino acid specific components of such viruses, such as the sequence encoding Sigma C capsid protein and to vaccines that protect birds from diseases caused by such viruses.

The invention discloses a bi-fluorescence quantitative RT-PCR detection kit for mycoplasma synoviae and avian reovirus. The kit comprises a primer group and a probe group, wherein the primer group comprises a primer 1, a primer 2, a primer 3 and a primer 4, which respectively have base sequences of sequence tables SEQ.ID.No.1 and 2, and SEQ.ID.No.4 and 5; and the probe group comprises a probe A and a probe B which respectively have the base sequences of sequence tables SEQ.ID.No.3 and 6. Experiments prove that the kit disclosed by the invention has the advantages of short detection time, high detection sensibility and strong specificity, two pathogens of the mycoplasma synoviae and the avian reovirus can be detected and discriminated at the same time, and the corresponding pathogeny content can be quantified, so that the kit can be used for assessing the curative effects of vaccines and medicines on the mycoplasma synoviae and the avian reovirus and researching pathogenic mechanisms and other respects; therefore, the kit disclosed by the invention has an important significance on prevention and control of the mycoplasma synoviae and the avian reovirus.

The invention provides application of a perylenequinone compound in the preparation of feed for preventing and treating poultry viruses, and belongs to the technical field of biological engineering and agriculture. The invention relates to the preparation of the feed for preventing and treating the poultry viruses such as Marek's disease virus, avian leukosis virus subgroup J, chicken infectious anemia virus, avian reovirus, newcastle disease virus, infectious bursal disease virus, goose parvovirus, duck plague virus, avian influenza virus and the like by adding the perylenequinone compound into poultry feed. The perylenequinone compound comprises hypocrellin, elsinochrome, polyanticin, cladosporin, calphostin, cercosporin and the like, and has a series advantages of being convenient to extract, having good stability, having no obvious side effect for oral administration, realizing quick metabolism and the like. The dosage of the perylenequinone compound in the preparation of the feedfor preventing and treating the poultry viruses is 1-50umol of perylenequinone compound per kg by weight every day; the dosage of the perylenequinone compound in the preparation of the feed for preventing and treating the poultry viruses is 50umol-200umol of perylenequinone compound per kg by weight; moreover, the perylenequinone compound has very obvious effect of preventing and treating the poultry viruses, meanwhile can be produced through modern biotechnology, and can bring good economic and social benefits.

The invention discloses a primer combination for identifying ChPV (Chicken Parvovirus) and ARV (Avian Reoviruses) and application of the primer combination. The primer combination consists of a primer pair I and a primer pair II, wherein the primer pair I consists of ChPV-F and ChPV-R which are respectively shown in sequence tables 1 and 2; the primer pair II consists of ARV-F and ARV-R which are respectively shown in sequence tables 3 and 4. The invention protects the application of the primer combination in identifying the ChPV and the ARV, the application of the primer combination in identifying whether a to-be-detected virus is ChPV or ARV or not and the application of the primer combination in identifying whether a to-be-detected sample is infected with the ChPV and / or the ARV or not. According to the primer combination for identifying the ChPV and the ARV and the application of the primer combination, disclosed by the invention, a duplex PCR (Polymerase Chain Reaction) established by the invention can be used for rapidly detecting mixed infection of the ChPV and the ARV and is suitable for large-batch detection, the cost and the time are saved, pollution is also reduced, and very high practical value is obtained.

The invention discloses a multiple fluoroimmunoassay primer, kit and method for rapidly distinguishing 5 avian immunosuppression pathogens. Operation is simple, a target amplified fragment is acquired through a PCR, an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized, the MFI value is read through a detector, and viruses of different types are distinguished. The method can simultaneously detect the chicken infectious anemia virus, the chicken Marek's disease virus, the avian reticuloendotheliosis virus, the avian reovirus and the chicken infectious bursa disease virus accurately, specificity is high, sensitivity is high, and repeatability is good. Compared with a traditional detection method, the method achieves simultaneous detection of multiple different target molecules in the same sample, the use number of samples is small, operation is simple and rapid, and the detection cost can be greatly reduced.