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Preparation method of avian reovirus multi-subunit vaccine

An avian reovirus and subunit vaccine technology, which is applied in the field of preparation of avian reovirus multi-subunit vaccines, can solve the problems of low immune efficacy of avian reovirus vaccines, and achieves increasing the range of antibody cross protection, Reduced immune side effects and good clinical protection

Inactive Publication Date: 2016-12-14
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a method for preparing an avian reovirus multi-unit subunit vaccine, so as to solve the technical problem of low immune efficacy of the avian reovirus vaccine in the prior art

Method used

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  • Preparation method of avian reovirus multi-subunit vaccine
  • Preparation method of avian reovirus multi-subunit vaccine
  • Preparation method of avian reovirus multi-subunit vaccine

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0021] A preparation method of avian reovirus multiple subunit vaccine, comprising the following steps:

[0022] 1) Prepare an aqueous solution containing 200 μg / ml σC protein of avian reovirus, 200 μg / ml λB protein of avian reovirus, and 200 μg / ml σB protein of avian reovirus to obtain a concatenin solution;

[0023] 2) Prepare an aqueous solution containing 55 mg / L cecropin and 55 mg / L muramyl dipeptide to obtain an immune enhancer;

[0024] 3) Take the concatenin solution obtained in step 1) and mix the immunopotentiator obtained in step 2), which is the vaccine aqueous phase;

[0025] 4) Take the vaccine oil phase, mix it with the vaccine water phase obtained in step 3), and then emulsify to obtain the avian reovirus multiple subunit vaccine.

[0026] On the basis of the above technical solutions, the following conditions are met:

[0027] In step 3), the mixing volume ratio of the concatenin solution obtained in step 1) and the immunopotentiator obtained in step 2) is 1...

Embodiment 2

[0045] A preparation method of avian reovirus multiple subunit vaccine, comprising the following steps:

[0046]1) Prepare an aqueous solution containing 300 μg / ml avian reovirus σC protein, 150 μg / ml avian reovirus λB protein, and 150 μg / ml avian reovirus σB protein to obtain a concatenin solution;

[0047] 2) Prepare an aqueous solution containing 100 mg / L cecropin and 100 mg / L muramyl dipeptide to obtain an immune enhancer;

[0048] 3) Take the concatenin solution obtained in step 1) and mix the immunopotentiator obtained in step 2), which is the vaccine aqueous phase;

[0049] 4) Take the vaccine oil phase, mix it with the vaccine water phase obtained in step 3), and then emulsify to obtain the avian reovirus multiple subunit vaccine.

[0050] On the basis of the above technical solutions, the following conditions are met:

[0051] In step 3), the mixing volume ratio of the concatenin solution obtained in step 1) and the immunopotentiator obtained in step 2) is 1:5.

[...

Embodiment 3

[0054] A preparation method of avian reovirus multiple subunit vaccine, comprising the following steps:

[0055] 1. The concentration of σC protein was purified and diluted according to conventional methods, and the concentration was 200ug / ml.

[0056] 2. The concentration of λB protein was purified and diluted according to conventional methods, and the concentration was 100ug / ml.

[0057] 3. The concentration of σB protein was purified and diluted according to conventional methods, and the concentration was 200ug / ml.

[0058] 4. The ratio of σC protein, λB protein and σB protein is 1:1:1.

[0059] 5. After mixing the three protein solutions, add cecropin with a final concentration of 50 mg / mL and 10 mg mg / mL of muramyl dipeptide.

[0060] 6. Fully mix the water phase in step 5, and emulsify with the imported oil adjuvant at a ratio of 1:2, and the emulsification time is 8 minutes.

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Abstract

The invention provides a preparation method of an avian reovirus multi-subunit vaccine. According to the technical scheme, prokaryotic expression is conducted aiming at group-specific neutralizing antibody antigens and type-specific neutralization reaction surface antigens mainly generated in a standard virus strain S1133 of an avian reovirus, all the antigens are matched according to a certain ratio, an immunopotentiator is added, an immune response of an organism can be fully stimulated, different antigen proteins cause the organism to generate different specific antibodies, the antibody cross protection range is expanded, and a good protection effect is achieved on clinical breeding of large poultry. The antigens in the vaccine prepared through the method are pure, inactivator is not used, and therefore immune side effects are reduced; meanwhile, the antibody level is high, clinical protection is good, the vaccine has the advantages of being low in cost and easy to produce on a large scale, and accordingly the vaccine has good popularization prospects.

Description

technical field [0001] The invention relates to the technical field of veterinary biological products, in particular to a preparation method of an avian reovirus multiple subunit vaccine. Background technique [0002] Avian reovirus is one of the main pathogens of chicken viral arthritis and tenosynovitis, belonging to Reoviridae, a kind of Reovirus genus. Since the strain was first isolated from the respiratory tract of chickens suffering from chronic respiratory diseases in 1954, a variety of avian reovirus isolates have been reported one after another. With the deepening of relevant research in recent years, it has been found that avian reovirus may cause other diseases besides viral joints and tenosynovitis. Avian reovirus was isolated from chickens with diseases such as syndrome and temporary digestive system disorders. [0003] Avian reovirus (ARV) belongs to the family Reoviridae and belongs to the genus Orthoreovirus. ARV genome dsRNA is wrapped with a double-laye...

Claims

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Application Information

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IPC IPC(8): A61K39/15A61P31/14
CPCA61K39/12A61K2039/552A61K2039/55516A61K2039/5555A61K2039/70C12N2720/12034
Inventor 冯玉斗李亚杰杨保收付旭彬李守军
Owner TIANJIN RINGPU BIO TECH
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