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Application of perylenequinone compound in preparation of feed for preventing and treating poultry viruses

A compound, poultry technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of hypericin cannot be effectively utilized, easy to decompose, and the chemical properties of hypericin are unstable.

Inactive Publication Date: 2012-12-05
江苏竹红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical properties of hypericin are unstable, and it is easy to decompose when exposed to light. In practical applications, a considerable part of hypericin cannot be effectively utilized.

Method used

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  • Application of perylenequinone compound in preparation of feed for preventing and treating poultry viruses
  • Application of perylenequinone compound in preparation of feed for preventing and treating poultry viruses
  • Application of perylenequinone compound in preparation of feed for preventing and treating poultry viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1: In vitro antiviral experiment of perylenequinone compounds

[0044] (1) Preparation of embryonic fibroblasts grown from chicken embryos for in vitro anti-virus experiments

[0045] Take chicken embryos about 11 days old and place them on an egg rack with the air cell facing up, sterilize with iodine for 5 minutes, and remove the eggshell in the air cell after deiodination with alcohol; take out the chicken embryo with sterile elbow tweezers Remove the head, claws and viscera in a bacterial plate; transfer the chicken embryo to a sterile small beaker, wash it with Hank's solution for 3 times, and cut it into about 0.5-1mm with small sterile scissors 3 Small pieces; wash 3 times with Hank's solution, add an appropriate amount of 0.25% trypsin, and digest in a 37°C water bath for about 10 minutes; add growth solution or serum to stop the reaction, blow gently with a pipette, add growth solution to resuspend; cell count, Pack cell flasks according to 4 million t...

Embodiment 2

[0072] Example 2: Anti-H5N1 virus experiment of solid-state fermented hypocrellin added to chicken feed

[0073] The basic dietary formula of chickens in the experiment is: corn 60%, millet 5%, sorghum 4.2%, wheat bran 5%, bean cake 8%, fish meal 8%, meat and bone meal 3%, blood meal 5%, stone powder 1.5%, salt 0.3 %. The basic daily ration is powder, which is crushed with a 1.8mm sieve.

[0074] Hypocretin is produced by solid-state fermentation, corn is the main raw material, and 2% glucose and 0.2% KH based on the weight of corn are added 2 PO 4 , 0.04% MgSO 4 ·7H 2 O. Add water and mix the material to make it wet, control the ratio of material to water at 1:0.7, sterilize at 121°C for 45-60 minutes, and add 10% liquid Shiraia sp.SUPER-H168 seeds after cooling. Both the initial pH and the pH of the fermentation process are natural, the fermentation temperature is 30°C, the humidity is controlled at 90% during the fermentation process, and the fermentation ends after 1...

Embodiment 3

[0086] Example 3 Anti-NDV virus experiment of liquid fermented hypocretin added to chicken feed

[0087] The experimental basal diet was the same as in Example 2.

[0088] Hypocretin is produced by liquid fermentation method, and the culture medium per liter consists of: glucose 20g, peptone 50g, potato 200g, (NH 4 ) 2 HPO 4 2g, KH 2 PO 4 2g, MgSO 4 0.5g / L, sterilized at 121°C for 20min, then add 10% liquid bamboo yellow fungus seeds after cooling. Both the initial pH and the pH of the fermentation process are natural, the fermentation temperature is 30° C., and the fermentation ends in 72 hours. The fermented liquid was removed by centrifugation to obtain mycelium, which was dried at 60°C. It was determined that hypocrellin A accounted for 8.1%, hypocrellin B accounted for 1.3%, and the total amount of other perylenequinone compounds accounted for 0.2%.

[0089] Select 80 1-day-old broilers with basically the same body condition (average body weight is 36g), and divide...

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Abstract

The invention provides application of a perylenequinone compound in the preparation of feed for preventing and treating poultry viruses, and belongs to the technical field of biological engineering and agriculture. The invention relates to the preparation of the feed for preventing and treating the poultry viruses such as Marek's disease virus, avian leukosis virus subgroup J, chicken infectious anemia virus, avian reovirus, newcastle disease virus, infectious bursal disease virus, goose parvovirus, duck plague virus, avian influenza virus and the like by adding the perylenequinone compound into poultry feed. The perylenequinone compound comprises hypocrellin, elsinochrome, polyanticin, cladosporin, calphostin, cercosporin and the like, and has a series advantages of being convenient to extract, having good stability, having no obvious side effect for oral administration, realizing quick metabolism and the like. The dosage of the perylenequinone compound in the preparation of the feedfor preventing and treating the poultry viruses is 1-50umol of perylenequinone compound per kg by weight every day; the dosage of the perylenequinone compound in the preparation of the feed for preventing and treating the poultry viruses is 50umol-200umol of perylenequinone compound per kg by weight; moreover, the perylenequinone compound has very obvious effect of preventing and treating the poultry viruses, meanwhile can be produced through modern biotechnology, and can bring good economic and social benefits.

Description

technical field [0001] The application of perylenequinone compounds in poultry feed belongs to the field of bioengineering and agricultural technology. The invention relates to the use of a perylenequinone compound added to poultry feed for the prevention and treatment of poultry viruses. Background technique [0002] In recent years, the occurrence and prevalence of infectious diseases have become an important factor hindering the rapid development of my country's animal industry. Especially viral diseases, a variety of viral infections may cause poultry immunosuppression. Such as Marek's disease virus (Marek's disease virus, MDV), avian reticuloendotheliosis virus (Reticuloendotheliosis virus, REV), avian J subgroup leukemia virus (Avian Leukosis virus subgroup J, ALV-J), chicken infectious anemia virus (Chicken infectious anemia virus, CAV), avian reovirus virus (ARV), Newcastle disease virus (NDV), infectious bursal disease virus (Infectious bursal disease virus, IBDV)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/17A23K1/18A23K50/70
Inventor 蔡宇杰廖祥儒朱建航丁彦蕊张大兵
Owner 江苏竹红生物科技有限公司
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