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Avian reovirus virus strain and application

A technology of avian reovirus and strains, applied in the directions of viruses, applications, viral peptides, etc., can solve the problem of single ARV isolates and other problems

Active Publication Date: 2020-02-07
天津渤海农牧产业联合研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the isolated strain of ARV is relatively single in the process of developing ARV vaccine at home and abroad at present, and to provide a novel avian reovirus virus strain

Method used

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  • Avian reovirus virus strain and application
  • Avian reovirus virus strain and application
  • Avian reovirus virus strain and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Isolation and identification of avian reovirus strains

[0020] 1. Isolation of avian reovirus strains

[0021] The experimental samples were collected from a large-scale chicken farm in Zhucheng, Shandong. The affected chickens were 30 days old, often lame, paralyzed, and even died. , Suspected chicken viral arthritis disease. After the joint fluid, joint swabs and tendons of the diseased chicken samples were ground, the supernatant was centrifuged and sterilized with a 0.22 μm filter, and then inoculated on the LMH cell plate that had been covered with a single layer, and a negative control was set at the same time. Place at 37°C, 5% CO 2 The cells were cultured in an incubator, and the cell status was observed every day. After the first generation of inoculation, no typical cell lesions appeared. After the cell plate was frozen and thawed three times, the virus liquid was harvested and the second generation was inoculated. 72 hours after virus inoculat...

Embodiment 2

[0042] Embodiment 2. Challenge protection test

[0043] 1. Use the S1133 strain to attack the virus

[0044]Seventy 14-day-old SPF chickens were randomly divided into 7 groups with 10 chickens in each group. Before the experiment, blood was collected from all the chickens to separate the serum, and the REOV antibody value was measured by ELISA, and the results showed that all the REOV antibodies were negative. Group 1-5 is the experimental immunization group, group 6 is the positive control group, and group 7 is the negative control group. Experimental immunization groups 1-5 are immunized against Qingdao Yibang chicken Newcastle disease, infectious bronchitis, and infectious bursa respectively. Disease, viral arthritis quadruple inactivated vaccine (AV2311 S1133 strain), Ruipu Biological Guanyantuo (ZJS strain) vaccine, MSD chicken viral arthritis inactivated vaccine (1733+2408 strain), clinical isolate REOV- For the inactivated vaccine prepared by 74 and SD0920, groups 1-3...

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Abstract

The invention provides an avian reovirus virus strain and application, and relates to the field of virus strain screening. The preservation number of the virus strain is CCTCC NO:V201971. According tothe avian reovirus virus strain and application, through isolation and identification, animal regression and challenge protection tests, it is found that the virus strain is a strong virus strain, and existing commercial vaccines or vaccines prepared from clinically isolated virus strains cannot produce effective protection or the protection rate is low, and the virus strain can be used for testing the efficacy of avian reovirus vaccine.

Description

technical field [0001] The invention belongs to the field of virus strain screening, and in particular relates to an avian reovirus strain and its application. Background technique [0002] Avian orthoreovirus (ARV) belongs to the family Reoviridae and the genus Orthoreovirus, and is a highly contagious avian virus. ARV infection can lead to arthritis, tenosynovitis, chronic malabsorption and malabsorption syndrome, etc. It can cause damage to the body's immune system, increase susceptibility to other pathogenic microorganisms, increase mortality, decrease feed conversion rate, decrease egg production, and reduce nutrition. The poultry industry causes huge economic losses. [0003] Studies at home and abroad have shown that in the process of vaccine development, because the clinical isolates of ARV are relatively single, only common classic strains such as S1133 strains are often used for efficacy testing. With the increase of ARV infection and the continuous mutation of th...

Claims

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Application Information

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IPC IPC(8): C12N7/00C12N15/46C07K14/08C12Q1/70C12Q1/686G01N33/569C12R1/93
CPCC12N7/00C07K14/005C12Q1/701C12Q1/686G01N33/56983C12N2720/12222G01N2333/14C12Q2521/107
Inventor 李守军马利芳冯敬敬陈家锃牛登云陈卓
Owner 天津渤海农牧产业联合研究院有限公司
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