80results about How to "Overcome size limitations" patented technology

The invention provides an isolated and purified DNA molecule comprising at least one DNA segment, a biologically active subunit or variant thereof, of a circular intermediate of adeno-associated virus, which DNA segment confers increased episomal stability, persistence or abundance of the isolated DNA molecule in a host cell. The invention also provides a composition comprising at least two adeno-associated virus vectors.

System and method for providing connection handoffs from macrocell to femtocell in wireless networks. A method comprises determining a group identifier from a received pseudorandom number sequence, determining a list of access points from a plurality of access points, wherein the access points in the list of access points are all identified by a group identifier, transmitting a request for detecting an identifier of the communications device to each access point in the list of access points, receiving a positive acknowledgement from a locating access point in the list of access points, and locating the communications device in a coverage area of the locating access point. The acknowledgement indicates that the locating access point successfully detected the identifier.

The invention provides an isolated and purified DNA molecule comprising at least one DNA segment, a biologically active subunit or variant thereof, of a circular intermediate of adeno-associated virus, which DNA segment confers increased episomal stability, persistence or abundance of the isolated DNA molecule in a host cell. The invention also provides a composition comprising at least two adeno-associated virus vectors.

The invention discloses a laser and microbeam plasma composite 3D (3-dimensional) printing equipment and method. The equipment comprises a central control system, a sealing forming chamber, a laser forming device and a plasma processing device, wherein a laser forming spray head and a plasma gun are correspondingly put on a support frame arranged at the left and right side inner walls of the sealing forming chamber, and the laser forming spray head or the plasma gun is clamped through a co-work numerical control main shaft, so that the laser forming spray head or the plasma gun moves in the Z, X and Y directions in the sealing forming chamber. The central control system controls the co-work numerical control main shaft for conveying back the laser forming spray head back to the support frame, then, the co-work numerical control main shaft clamps the plasma gun for filling the inside of the profile of a formed metal part, and the work is alternately carried out in such a way until the part manufacturing is completed, so that the scanning time is reduced to a great degree. The laser fast forming is combined with the plasma, the problem of conflict between the efficiency and the precision of a metal 3D printing technology is solved, and in addition, the plasma is adopted for replacing the laser to be used as a heat source, so that the high-efficiency and low-cost industrial level metal 3D printing equipment is obtained.

A Cas9 expression plasmid, a genome editing system and a genome editing method for Escherichia coli are provided. The Cas9 expression plasmid includes a tracrRNA sequence, a Cas9 gene and a chloramphenicol resistance gene (CmR). The Cas9 expression plasmid is applied to CRISPR / Cas-coulped λ-red recombineering system for editing genomes of E. coli with high efficiency.

The invention provides recombinant AAV vectors, at least one of which has heterologous terminal palindromic sequences, and methods of using those vectors.

The invention discloses a material fatigue performance obtaining method for a sheet sample, which comprises the following steps: step 1: completing a tension-pressure symmetric cyclic loading test ofa multi-stage strain amplitude for the sheet sample under strain control to obtain a cyclic stable load-displacement curve; step 2: connecting load-displacement curve hysteresis loop tips as the cyclic load-displacement curve, and predicting a cyclic stress-strain relationship in accordance with a Ramberg-Osgood constitutive model with a cyclic load-displacement relationship; step 3: establishinga relationship between a true strain amplitude epsilon r, a stress amplitude sigma r and a measured and controlled strain amplitude epsilon eq of a fatigue source RVE by using the cyclic stress-strainrelationship as a material parameter; step 4: establishing a fatigue life estimation model according to epsilon r and sigma r to obtain the fatigue property of the material. According to the materialfatigue performance obtaining method, the material dimension limitations of the traditional fatigue performance test method are overcome, and the method does not rely on empirical formulas, and is applied to different materials and sample configurations.

The invention discloses a non-contact device for online measurement of the outer diameter of a piston pin. A light source, a first lens, apertures, a second lens, a V-shaped groove, an objective lens and a planar CCD are sequentially disposed on a guide track and positioned on the same optical axis, a measured piston pin is disposed inside the V-shaped groove, and the planar CCD is connected with a computer via a data acquisition card. The measured piston pin is provided with uniform parallel lights by a Kohler illumination system to allow the upside contour of the measured piston pin to be imaged on the planar CCD sensor, and the outer diameter of the piston pin can be determined by image processing and calibrating the imaging part of the piston pin, so as to overcome the limitation of the CCD area to the size of the measured piston pin. Meanwhile, the invention uses sub-pixel image processing method, so as to overcome the influence of the CCD resolution to measurement accuracy. The invention has the advantages of rapid operation, remarkably improved measurement efficiency, and remarkably improved measurement accuracy arising from the sub-pixel image processing method.

The invention discloses a reinforcement structure for arch-covering-method underground excavation subway station arch feet and a construction method. The reinforcement structure for the arch feet comprises two layers of arch cover supports, arch foot piles distributed at the arch feet of the left and right sides of subway station arch cover bodies and arch foot longitudinal beams, the arch foot longitudinal beams and the arch foot piles are integrally connected, and pile bodies of the arch foot piles are distributed along the tangent line of the arch feet; each arch foot pile is composed of asection steel framework and fine aggregate concrete which is poured into the section steel framework and pile holes. The reinforcement structure is a reinforcement system combining the arch foot pileswith the longitudinal beams, the longitudinal beams are connected with the arch foot piles and the first and second layers of arch cover supports, the structural integrity is good, the capacity of resisting local stress and deformation is high, and deep and thick smashed surrounding rock at the arch feet can be effectively reinforced and protected; in addition, according to the construction method of the reinforcement structure for the arch feet, the reinforcement range and implementation objects are flexibly selected, rock layers of the arch feet for supporting arch covers are smashed, the integrity influence cannot be caused, and the advantages of an arch-cover method in the aspects such as project cost, construction working period, implementation efficiency and mechanical operation cannot be influenced.

The invention provides an isolated and purified DNA molecule comprising at least one DNA segment, a biologically active subunit or variant thereof, of a circular intermediate of adeno-associated virus, which DNA segment confers increased episomal stability, persistence or abundance of the isolated DNA molecule in a host cell. The invention also provides a composition comprising at least two adeno-associated virus vectors.

A single-sided continuous optoelectrowetting (SCOEW) device for manipulating droplets retained in a fluid over the SCOEW device with dynamic patterns of low intensity light, such as from a display screen, is described. A single pair of lateral electrodes are utilized for providing a lateral electric field bias, with transport motion controlled in response to projecting light through a photoconductive layer and dielectric layer adjacent to which droplets are retained. The device is configured for optically manipulating droplets having volumes spanning over five orders of magnitude, and can be configured to perform droplet dispensing, transport, splitting, merging, mixing and other droplet manipulation functions involving any of the above on a single sided surface.

A method and system for providing an RFID transponder (200) with a fabric antenna (50). The system includes a radio frequency (RF) transponder having a housing (30) in which RF circuitry (5) is encapsulated and a fabric antenna coupled to the RF circuitry. A method of manufacturing a radio frequency (RF) transponder includes the steps of enclosing an RF circuit in a housing, forming a fabric antenna, and coupling the fabric antenna to the RF circuit.

The invention relates to a method for increasing the specific capacitance of a graphene-based supercapacitor. An electrode of a supercapacitor is prepared from graphene, and the theoretical capacitance can be up to 550F / g. However, because of factors such as material properties and preparation techniques, generally the contained specific capacitance is within 150-300F / g. According to the method, sodium chloride and urea are doped into a graphene oxide solution of a liquid crystal state for the first time, a film is prepared from the solution by using a scraper in a coating manner, and thus a film obtained after hydrothermal reduction is adopted as the electrode of the supercapacitor. Due to added substances, graphitization stacking of graphene layers can be prevented and nitrogen doping can be achieved in the hydrothermal reduction process of the graphene film, so that the specific capacitance can be greater than 400F / g when aqueous electrolyte is used, and is at a relatively high level. The electrode of the graphene-based supercapacitor prepared by using the method is uniform in thickness, good in operability and simple to operate, in addition, the used raw materials are cheap, and the method is green. The process of the scraper coating method for preparing the film can be further improved, and can be applied to industrial production.

A funnel in combination with a commercial appliance, such as a refrigerator or icemaker, that will allow a product, such as water or ice, to be redirected and dispensed into a container that would otherwise be too large to fit into a recessed dispensing cavity typically allotted for smaller containers. To accomplish this, a sleeve member is operably coupled to the appliance in either a friction-fit arrangement or a mounting arrangement at or around an optimal deflection angle of 30°, thus allowing the water or ice to overcome a lip on the dispensing cavity and be redirected into a larger container.

The invention provides an isolated and purified DNA molecule comprising at least one DNA segment, a biologically active subunit or variant thereof, of a circular intermediate of adeno-associated virus, which DNA segment confers increased episomal stability, persistence or abundance of the isolated DNA molecule in a host cell. The invention also provides a composition comprising at least two adeno-associated virus vectors.

The invention discloses a particle extruding and forming mechanism provided with concave-convex pattern roller pairs. The particle extruding and forming mechanism comprises a handpiece base, a transmission mechanism and an extruding mechanism, wherein the transmission mechanism and the extruding mechanism are arranged on the handpiece base. The extruding mechanism comprises a pair of extruding rollers used for extruding raw materials. The transmission mechanism is connected with the extruding rollers and drives the extruding rollers to move in the opposite directions. Drums of the extruding rollers are hollow drums. A plurality of rows of protruding structures extending in the longitudinal directions of the drums are uniformly arranged outside the ends, connected with the transmission mechanism, of the two drums. The protruding structures in every two adjacent rows make mutual contact. Each protruding structure in each row comprises a plurality of protruding tops equally spaced. The protruding tops form the outer surfaces of the extruding rollers together. A die hole is formed in a depression between every two protruding tops. According to the particle extruding and forming mechanism, power consumption is small, the defect that the size of particles is limited is overcome, the strength of space gaps formed by the protruding tops is sufficient, the technological problem of hole wall cracking caused by stress concentration in the heat treatment process is solved, the service life of the extruding rollers is prolonged, and cost is saved.

The invention discloses a text input method for a smart watch. The text input method comprises the following steps: detecting one or more groups of sensor data sequences caused by user handwriting through an inertial sensor; acquiring character information corresponding to each group of sensor data sequences one by one according to the sensor data sequences, wherein each group of the character information comprises M candidate characters; acquiring a plurality of different candidate texts according to the character information; and selecting a text matched with the user handwriting from the plurality of candidate texts as the text recognized by the smart watch. Accordingly, the invention also discloses a text input device for the smart watch. The text input method and the text input device provided by the invention do not need to set a keyboard on a touch screen, overcomes the limitation on the size of the touch screen, also saves the space for displaying the keyboard, can have larger space for displaying other contents, and overcomes requirements of voice input on surroundings, so that the text input is not restricted by occasions.

The invention relates to high-strength and high-toughness wear-resistant steel and a preparation method for nano twin crystal reinforcement and toughening of high-strength and high-toughness wear-resistant steel. The steel comprises the components in percent by mass: 0.35%-0.85% of C, 15.2%-24.8% of Mn, 0.1%-0.5% of Si, smaller than or equal to 1.0% of Al, smaller than or equal to 0.005% of S, smaller than or equal to 0.01% of P, 0.5%-1.5% of Nb, 0.2%-0.8% of Ti, 0.2%-0.75% of V, 0.005%-0.05% of N, 0.02%-0.1% of rare earth RE and the balance Fe and inevitable impurities. The stacking fault energy of an alloy is 24-30 kJ / m <2>, and the mass percent of the addition amount of Ti, Nb and V is 2: 1: 1. The preparation process comprises the steps of alloy smelting and negative pressure casting, homogenization heat treatment and cogging, hot rolling, nanometer twinning treatment, nanometer twinning stabilization and low-temperature aging treatment. According to the invention, fine structure regulation and control are carried out based on means of deformation and heat treatment by means of low fault energy and microalloying component optimization matching design, so that good structure matching is realized, and the alloy has high strength, high toughness and high wear resistance. The wear-resistant steel is excellent in comprehensive mechanical property, low in alloy cost, simple in preparation process, low in production and manufacturing cost and easy to put into industrial production and large-scale industrial equipment.

The invention relates to a chip back side drape tin eutectic technology and a loading method thereof. The method comprises the following steps: step (1), carrying out backside silver or backside gold processing on a chip back side; step (2), carrying out tinning or tin alloy work on a backside silver surface or a backside gold surface of a chip which is subjected to the backside silver or backside gold processing in the step (1); step (3), cutting the chip whose backside silver surface or backside gold surface is subjected to the tinning or tin alloy work in the step (2); step (4), employing eutectic technology to install the chip which is cut in the step (3) to a basic island to complete loading. According to the chip back side drape tin eutectic technology and the loading method, there is no requirement to chip dimension, after the chip back side is coated with a layer of tin or tin alloy, since the thickness of the tin or tin alloy can be controlled, the thickness of the tin or tin alloy can effectively absorb different stresses between the chip and the basic island, and lamination and rupture problems of a traditional eutectic chip are solved.

The invention relates to a wall-embedded smart five-hole socket, and belongs to the technical field of sockets. The wall-embedded five-hole smart socket comprises a terminal fixing plate arranged in a bottom box, two relays, a five-hole socket inner core, a switching power supply module, a power detection PCB and a CPU main control board. In order to meet the requirements of a green energy-saving smart community advocated by the state at present, internal structure layout and functions of multiple existing external five-hole sockets and embedded five-hole sockets are combined, so that individual controlling and measurement and quantitative statistics of a remote smart terminal (mobile phone) on a two-hole jack and a three-hole jack of the smart five-hole socket are achieved, the utilization rate of the internal space of the bottom box is also improved, various control components are reasonably and compactly arranged in the bottom box, the size limitation of a national standard 86 box is overcome and the practicality and the universality of the embedded smart five-hole socket are improved.

The invention discloses a butt welding auxiliary device for single-side welding and double-side forming of a small-size rectangular pipe and a construction method. The auxiliary device comprises a body framework, a ceramic liner, a ceramic liner guide module and a limiting device. The limiting device comprises four tabulate limiting plates arranged on the upper, lower, left and right sides of a supporting framework, each limiting plate is connected with the supporting framework through multiple elastic connecting pieces, the ceramic liner guide module is a tapered square shell, the small end of the ceramic liner guide module is away from the ceramic liner, and the other end of the ceramic liner guide module is fixedly connected with the ceramic liner. According to the construction method,the ceramic liner is fixed to the inner side of a weld joint of a first rectangular pipe and a second rectangular pipe through the auxiliary device. The auxiliary device is high in assembling degree,and components of the auxiliary device are directly installed and used on a welding site after being prefabricated, machined and assembled in a factory. The assembling degree is high, and the weldingconstruction efficiency is improved.

The invention provides a long-span rotor system and a gas turbine generator set. The rotor system comprises a rotating shaft which is of an integrally formed structure. A thrust bearing, a first radial bearing, a motor, a gas compressor, a second radial bearing and a turbine are successively arranged on the rotating shaft. In the rotor system, two supporting points (the first radial bearing and the second radial bearing) are placed at two ends of the rotating shaft, separately, so that the rotating shaft is better in equilibrium and the rotor system is good in operating stability. The long-span rotor system can solve the problems of dimensional limit of the tail of the rotating shaft and unstable operation and severe vibration of the rotor system.

The invention provides a forming method for an airspace engine spray tube part. The method comprises the following steps: step I, cutting out a plurality of coaxial circular table rings on a plate; step II, performing spinning and shape-correcting on the circular table rings with too great dimensions or similar dimensions; step III, turning out circular ring surfaces which are welded with one another at large hole ends and small hole ends of all circular table rings; and step IV, sequentially welding the circular table rings into an integer according to the dimensions of the large hole ends from small to big or from big to small. The forming method for the airspace engine spray tube part provided by the invention has the advantages of being simple in process, not limited by an axle diameter ratio and a dimension, being capable of detecting and measuring the process, capable of being integrally formed, being high in production quality stability, being suitable for large-batch automaticproduction, and the like.