44results about How to "Many characteristic peaks" patented technology

The invention provides a method for establishing a fingerprint of leech. The method comprises the following specific steps: preparing the leech into a test solution; and performing separation detection through a high performance liquid chromatograph under certain conditions to obtain the fingerprint of the leech. The invention also provides a standard fingerprint of the leech, which is obtained through the method. The fingerprint can provide a reliable basis for leech identification and internal quality control. The method is simple and convenient to operate, high in stability, high in repeatability and large in quantity of characteristic peaks, and is capable of comprehensively and accurately evaluating the quality of leech medicine.

The invention discloses an establishment method of inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints specifically comprises the steps of preparing inducing callus cultured Allium sativum L. bulbs into solution for test products through steps such as solid-phase extraction, and performing high-performance chromatographic analysis and detection under certain conditions to obtain HPLC fingerprints of inducing callus cultured Allium sativum L. enzymatic hydrolysate. By analyzing the HPLC fingerprints of 10 batches of samples, characteristic common peaks are determined and standard fingerprints are obtained. The standard fingerprints can provide a reliable basis for variety identification and production quality control of inducing callus cultured Allium sativum L. The establishment method of the inducing callus cultured Allium sativum L. enzymatic hydrolysate HPLC fingerprints has the advantages that the operation is convenient, the stability is high, the specificity is strong, the repeatability is good, the characteristic peaks are more, the analysis efficiency is high, the truth identification of the inducing callus cultured Allium sativum L. can be realized by adopting the invention, and the interior product quality can be comprehensively, accurately and effectively evaluated.

The invention provides an establishing method and a use of a flue-cured tobacco GC / MS fingerprint. The establishing method of the flue-cured tobacco GC / MS fingerprint comprises the following steps of 1, preparing a solution needing to be detected, 2, acquiring the flue-cured tobacco GC / MS fingerprint, 3, establishing a flue-cured tobacco fingerprint common peak database, and 4, carrying out cluster analysis of the flue-cured tobacco fingerprint common peak database so that tobacco quality grades are determined. The establishing method of the flue-cured tobacco GC / MS fingerprint can objectively show flue-cured tobacco quality, replaces a traditional artificial evaluation method and a common chemical determination method, reduces work intensity of evaluation workers, improves tobacco quality evaluation scientificalness, and has an important meaning for improvement of flue-cured tobacco production economic benefits. The establishing method of the flue-cured tobacco GC / MS fingerprint is characterized in that aiming at flue-cured tobacco fragrant components, the establishing method utilizes an ultrapure water system-based distillation-extraction synchronized technology for extraction and a GC / MS detection technology so that the flue-cured tobacco GC / MS fingerprint having a good baseline separation effect, a lot of characteristic peaks and a high peak responding degree is established. The invention aims at applying the established flue-cured tobacco GC / MS fingerprint and its matching analysis technology in the fields of flue-cured tobacco quality grading, flue-cured tobacco producing area identification and tobacco leaf quality control in blend threshing.

The invention provides an establishing method of the fingerprint of a traditional Chinese medicinal composition, and a technology for detecting the components of the traditional Chinese medicinal composition by using the fingerprint established through the method. The raw materials of the traditional Chinese medicinal composition comprise Rhizoma Cibotii, Fructus Rosae Laevigatae, Caulis Spatholobi, Philippine flemingia root, Kadsura coccinea, Millettia specisoa Champ, steamed Ligustri Lucidi Fructus, steamed Chinese Taxillus Twig, brine processed semen cuscutae, processed Rhizoma Corydalis, Zanthoxylum nitidum, processed Boswellia carteri and processed myrrh. The establishing method of the fingerprint is characterized in that the retention time of every compound and every medicinal material in the traditional Chinese medicinal composition is detected through high performance liquid chromatography; and the conditions of the high performance liquid chromatography are as follows: octadecylsilane bonded silica is adopted as a stationary phase, a mobile phase comprises acetonitrile and an aqueous phosphoric acid solution with the weight percentage concentration of 0.1%, the volume percentage of the acetonitrile in the mobile phase uniformly increases to 90% from 5% at 0-170 min, the flow velocity is 1 ml / min, the column temperature is 30 DEG C, and the ultraviolet detection wavelength is 254 nm.

The invention provides an establishment method of a scorzonera fingerprint spectrum. The establishment method comprises the following specific steps: preparing a test solution by scorzonera, and carrying out fractionation detection under a certain condition by a high performance liquid chromatograph to obtain the scorzonera fingerprint spectrum. The invention further provides a scorzonera standard fingerprint spectrum obtained by the method. The standard fingerprint spectrum can provide reliable basis for scorzonera identification and internal mass control. The establishment method is simple and convenient to operate, high in stability, good in reproducibility and multiple in characteristic peaks. By utilizing the establishment method, the quality of scorzonera medicinal materials can be comprehensively and accurately evaluated.

The invention discloses a method for establishing the HPLC fingerprint of Tingli Dazao Xiefei Granules. The method comprises the preparation of the test solution, the determination of the HPLC chromatographic conditions and the preparation of the HPLC standard fingerprint. The invention also discloses the HPLC standard fingerprint spectrum of the Tinglidazao Xiefei granule obtained by the method, and the fingerprint spectrum has 27 common peaks. The quality detection method of the present invention is easy to operate, has high stability and good reproducibility, and the obtained spectrum has many characteristic peaks. Through the comparison of the common peaks of the standard fingerprint spectrum, the quality of Tingli Dazao Xiefei Granules can be comprehensively evaluated and controlled. It is beneficial to accurately evaluate the internal quality of preparations and ensure the safety and effectiveness of clinical medication.

The invention discloses an establishment method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of an allinase inactivated extract of six-peal red garlics. The establishment method comprises the following specific steps of preparing six-peal red bulbs which belong to a peculiar variety in Baodi County into a test product solution by a solid-phase extraction step and the like; performing HPLC detection under certain conditions to obtain the HPLC fingerprint spectrum of the allinase inactivated extract of the six-peal red garlics; analyzing the HPLC fingerprint spectrums of ten batches of samples to determine a common characteristic peak and obtaining a standard fingerprint spectrum which can provide a reliable basis for the identification of varieties and production places of the six-peal red garlics and control over the production quality. The establishment method disclosed by the invention has the advantages of convenience in operation, high stability, high specificity, good reproducibility, more characteristic peaks and high analytic efficiency; by using the establishment method disclosed by the invention, the authenticity and production place of the six-peal red garlics can be identified, and the internal quality of the six-peal red garlics can be evaluated comprehensively, accurately and effectively.

The invention relates to the technical field of quality control of traditional Chinese medicine preparations, and specifically discloses an HPLC fingerprint of hawthorn broken-wall decoction pieces, a construction method and application thereof. The construction method comprises the steps of preparation of test solution, preparation of reference solution, determination of HPLC analysis chromatographic conditions, determination of fingerprint and the like. In the present invention, by comparing the HPLC fingerprints of samples of 10 batches of hawthorn broken wall decoction pieces, the common peaks are determined to obtain the standard HPLC fingerprints. The construction method of the invention has the advantages of simple operation, good reproducibility, many characteristic peaks, accuracy and reliability. The HPLC fingerprint of the invention can more comprehensively reflect the characteristic peak information of the sample, and the quality control of the broken-wall decoction pieces of hawthorn can be carried out by using the HPLC fingerprint.

The invention discloses a method for constructing the fingerprint of Tianmeng Oral Liquid and its application. The method includes: preparation of the test solution, preparation of the reference solution, optimization of chromatographic conditions, measurement and analysis of the obtained spectrum by fingerprint software to process. The present invention adopts chromatographic columns of different stationary phases to be connected through a six-way valve, and the same mobile phase is gradiently eluted, and a variety of characteristic index components are well separated. As many characteristic components as possible are separated and detected, and more characteristic peaks of fingerprints are obtained, which can more comprehensively reflect the component information of Tianmeng Oral Liquid. Good and other advantages, it can more comprehensively and scientifically evaluate the quality of Tianmeng Oral Liquid, so as to ensure the quality and curative effect of the product, and provide a reference for the improvement of its quality standards.

The invention discloses a method for establishing the HPLC fingerprint of a Liubanhong garlic enzymatic hydrolysis product. The method concretely comprises the following steps: carrying out solid phase extraction on bulbs of Liubanhong garlic which is a special kind in Baodi to prepare a test article solution, and carrying out high performance liquid chromatography analysis detection under certain conditions to obtain the HPLC fingerprint of the Liubanhong garlic enzymatic hydrolysis product. A standard fingerprint is obtained by determining common characteristic peaks HPLC fingerprint analysis of 10 batches of samples. The standard fingerprint can provide reliable bases for discrimination of the kind and the producing area of the Liubanhong garlic and production quality control. The method has the advantages of convenient operation, high stability, strong specificity, good reappearance, many characteristic peaks, high analysis efficiency, true and false and producing area discrimination of the Liubanhong garlic, and comprehensive, accurate and effective evaluation of the internal quality of the Liubanhong garlic.