Construction method and application of standard characteristic chromatogram of Chinese patent medicine pharyngitis-treating tablet

Abstract

The invention relates to a construction method and application of a standard characteristic chromatogram of a Chinese patent medicine, i.e., a pharyngitis-treating tablet. The construction method comprises the following steps: weighing control substances of (R,S)-goitrin, paeoniflorin and harpagoside, a sample of the pharyngitis-treating tablet, and 12 negative samples of the pharyngitis-treating tablet, wherein the 12 negative samples are prepared according to a prescription and preparation process for the pharyngitis-treating tablet and respectively lack in Radix Scrophulariae, isatis roots, Radix Asparagi, Radix Ophiopogonis, tree peony bark, Radix Stemonae Preparata, Chinese olive, Flos Farfarae Preparata, Oroxylum indicum, Rhizoma Rehmanniae, Periostracum Cicadae and peppermint oil; separately preparing control solutions, a test solution and negative sample solutions; precisely sucking up 1 [mu]L of each of the above solutions and injecting the sucked solutions into a liquid chromatograph; carrying out determination according to set chromatographic conditions so as to obtain corresponding chromatograms; and carrying out calculating by using a chromatographic fingerprint similarity evaluation system for traditional Chinese medicines so as to generate the standard characteristic chromatogram of the pharyngitis-treating tablet. The standard characteristic chromatogram of the pharyngitis-treating tablet constructed in the invention for the first time is complete in characteristic peaks, accurate in positioning and fast in construction method, can effectively identify the authenticity of the Chinese patent medicine and evaluate the quality of the Chinese patent medicine, and is expected to become a standard method for quality control of the Chinese patent medicine, i.e., the pharyngitis-treating tablet.

Description

technical field [0001] The invention relates to the field of quality detection of traditional Chinese medicines, in particular to a construction method and application of a standard characteristic map of Chinese patent medicine Yanyan Tablets. Background technique [0002] Pharyngitis Tablets is a traditional Chinese medicine preparation composed of twelve traditional Chinese medicine decoction pieces such as Scrophularia scrophulariae, Radix Isatidis, Radix Asparagus, Ophiopogon japonicus, Moutan bark, Zhibaibu, Qingguo, Zhishaoshui, Wood butterfly, Rehmannia glutinosa, cicada slough, and peppermint oil. The chemical composition is very complex. The preparation has the effects of nourishing yin and moistening the lungs, clearing heat and detoxification, clearing the throat, relieving cough and relieving itching. It is used for dry throat, itchy throat and irritating cough caused by chronic pharyngitis. The preparation method is as follows: except peppermint oil, coltsfoot...

AI Technical Summary

Problems solved by technology

[0003] The existing characteristic map technology is a comprehensive detection method that can be applied to the components of traditional Chinese medicine. It is through the detection of common characteristic peaks to achieve the purpose of detecting multiple components in the sample to be tested. However, there is no overall There is no effective detection method for its quality control, such as relying on thin-layer chromatography to detect part of the medicinal taste of the Chinese patent medicine in Yanyan Tablets, or using high-performance liquid chromatography for one or more components in the Chinese patent medicine of Yanyan Tablets -Ultraviolet detector method for content determination, one-sided reflection of the quality of Chinese patent medicines in Yanyan Tablets has serious defects, such as Qin Xiangqin et al. For the content of paeoniflorin in Yanyan Tablets, only the content of one component contained in Cortex Moutan in Yanyan Tablets was determined

Fu Yonghui et al. (Journal of Drug Analysis. 29 (12): 2032-2035) measure the content of 5 kinds of index components in Yanyan Tablets, but can only measure the content of 5 kinds of qualitative components such as harpagoside, but cannot determine the contents of Yanyan Tablets. Therefore, it can only reflect the quality of Yanyan Tablets one-sidedly, not only cannot comprehensively and effectively measure the quality of Yanyan Tablets, but also cannot accurately and quickly judge the authenticity of Yanyan Tablets

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