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Establishing method and use of flue-cured tobacco GC/MS fingerprint

A technology of fingerprint spectrum and establishment method, applied in the field of tobacco quality grading, can solve problems such as no patent application in the application field, and achieve the effects of improving production level and economic benefit, good baseline separation, and high peak responsivity

Active Publication Date: 2013-12-25
YUNNAN TOBACCO LEAF
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore the method that this patent adopts and field of application have not yet seen patent application

Method used

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  • Establishing method and use of flue-cured tobacco GC/MS fingerprint
  • Establishing method and use of flue-cured tobacco GC/MS fingerprint
  • Establishing method and use of flue-cured tobacco GC/MS fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for establishing a flue-cured tobacco GC / MS fingerprint of the present invention is realized through the following steps:

[0032] 1) Preparation of the solution to be tested: Connect a 500mL flat-bottomed flask to one end of the distillation extraction device, put 20.00g of tobacco powder into the flat-bottomed flask, add 350mL of pure water and 0.3mL of phenylethyl propionate with a concentration of 4μL / mL, and heated with an electric heating mantle that can control the voltage; connect a 100mL flask to the other end of the distillation extraction device, put 50mL of dichloromethane in the flask, place the flask on a water bath for heating, and distill at 56°C. Extract for 2.5 hours, remove the 100mL flask, add 5g of anhydrous sodium sulfate to it, seal it overnight, then put it into a rotary evaporator to concentrate to 1mL, pass through a 0.45μm filter membrane, and the filtrate is the solution to be tested;

[0033] 2) Acquisition of flue-cured tobacco GC / ...

Embodiment 2

[0037] The tested tobacco variety K326, grade C3F, was obtained from 15 different tobacco leaf producing areas in Yunnan Province. All the tobacco samples were collected in 2009 and made into shredded tobacco after uniform redrying. Cut tobacco samples were dried at 40°C, pulverized with a plant grinder, passed through a 60-mesh sieve, sealed with a plastic bag, and stored in an ultra-low temperature refrigerator (-80°C) for testing. The determination method is as follows:

[0038] 1) Preparation of the solution to be tested: Connect a 500mL flat-bottomed flask to one end of the distillation extraction device, put 20.00g of tobacco powder into the flat-bottomed flask, add 450mL of pure water and 0.6mL of phenylethyl propionate with a concentration of 4μL / mL, And heat the flat-bottomed flask with a voltage-controllable electric heating mantle; connect a 100mL flask to the other end of the distillation extraction device, put 80mL of dichloromethane in the flask, place the flask ...

Embodiment 3

[0043] The tested tobacco variety K326, with a grade of B2F, was obtained from 17 different tobacco leaf producing areas in Yunnan Province. Analyze according to the preparation method and analysis method of Example 1, draw the clustering diagram of different samples ( Figure 4 ).

[0044] by cluster analysis Figure 4 It can be seen that when the distance is about 0.05, the B2F grade tobacco leaves of various production areas in Yunnan Province in 2009 can be divided into 6 categories: the first category of tobacco leaf production areas is Shizong, Luoping, Honghe; the second category is Puer, Wenshan, Lu Liang, Lincang; the third category is Dali, Zhaotong, Baoshan; the fourth category is Xundian, Chuxiong, Fuyuan, Zhanyi; the fifth category is Qilin, Yuxi; and Fuyuan is the sixth category.

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Abstract

The invention provides an establishing method and a use of a flue-cured tobacco GC / MS fingerprint. The establishing method of the flue-cured tobacco GC / MS fingerprint comprises the following steps of 1, preparing a solution needing to be detected, 2, acquiring the flue-cured tobacco GC / MS fingerprint, 3, establishing a flue-cured tobacco fingerprint common peak database, and 4, carrying out cluster analysis of the flue-cured tobacco fingerprint common peak database so that tobacco quality grades are determined. The establishing method of the flue-cured tobacco GC / MS fingerprint can objectively show flue-cured tobacco quality, replaces a traditional artificial evaluation method and a common chemical determination method, reduces work intensity of evaluation workers, improves tobacco quality evaluation scientificalness, and has an important meaning for improvement of flue-cured tobacco production economic benefits. The establishing method of the flue-cured tobacco GC / MS fingerprint is characterized in that aiming at flue-cured tobacco fragrant components, the establishing method utilizes an ultrapure water system-based distillation-extraction synchronized technology for extraction and a GC / MS detection technology so that the flue-cured tobacco GC / MS fingerprint having a good baseline separation effect, a lot of characteristic peaks and a high peak responding degree is established. The invention aims at applying the established flue-cured tobacco GC / MS fingerprint and its matching analysis technology in the fields of flue-cured tobacco quality grading, flue-cured tobacco producing area identification and tobacco leaf quality control in blend threshing.

Description

Technical field [0001] The present invention is the field of tobacco quality grading, which involves a kind of analysis method for the quality analysis of roasting tobacco, the brand identification of the tobacco brand, and the quality analysis method in the leaf formula. technical background [0002] As a natural plant, tobacco, its composition is quite complicated. At present, it has been separated from more than 5,000 aromatherapy.These ingredients have a large or small impact on their quality. If they are analyzed according to the conventional chemical testing, they only analyze their main chemical ingredients, so that the quality of tobacco is obviously biased.Therefore, the identification of tobacco quality now depends mainly on manual evaluation.Although artificial evaluation can be grasped as a whole, it is easily affected by subjective factors such as personal emotions and sensory differences.Sometimes there are large deviations and a large workload. [0003] The fingerp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 矣跃平袁仕信何云燕刘磊尹晓东曹林海涂书新何结望阎铁军李琳
Owner YUNNAN TOBACCO LEAF
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