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Construction method and application of fingerprint of sweet dream oral liquid

A sweet dream oral liquid and fingerprint technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as limited resolution and peak capacity, insufficient and comprehensive component information, achieve more characteristic peaks, and improve separation options The effect of sex and method is simple and easy to implement

Active Publication Date: 2019-12-10
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventor further researched and found that for Tianmeng oral liquid with complex components, the resolution and peak capacity of the above method are still relatively limited, reflecting that the component information is not rich and comprehensive enough.

Method used

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  • Construction method and application of fingerprint of sweet dream oral liquid
  • Construction method and application of fingerprint of sweet dream oral liquid
  • Construction method and application of fingerprint of sweet dream oral liquid

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] (1) Preparation of the test solution: take Tianmeng Oral Liquid, add methanol, ultrasonically extract, return to room temperature, centrifuge, take the supernatant and filter, and take the continued filtrate to obtain the test solution;

[0041] (2) Preparation of the reference substance solution: take the reference substances of eleutheroside B, chlorogenic acid, acteocoside, hesperidin and icariin, dried under reduced pressure to constant weight, and add methanol respectively to prepare the reference substance solution ;

[0042] (3) Determination: Accurately measure the need testing solution that step (1) prepares and the reference substance solution that step (2) prepares respectively, inject high-performance liquid chromatograph and measure, mobile phase consists of acetonitrile-0.2% phosphoric acid aqueous solution , using gradient elution, the chromatographic column is connected by C8 and C18 through a six-way valve, and the chromatogram is recorded;

[0043] (4...

Embodiment 1

[0067] Example 1: Construction and application of the fingerprint of Tianmeng Oral Liquid based on column switching

[0068] The chromatographic conditions are: the chromatographic column is connected by ACE EXCEL (250×4.6mm, 5μm) and JADE-PAK (50×4.6mm, 3.5μm) through a six-way valve; the connection method 1 is double columns in the first 35min, and a six-way valve switch to 1→2 (such as figure 1 A), after 35 minutes, it becomes a single column, and the six-way valve is switched to 1→6 (such as figure 1 B), at this time, the composition before 35 minutes is monitored; the connection mode 2 is a single column for the first 35 minutes, and the six-way valve is switched to 1→2 (such as figure 1 C), after 35 minutes, it becomes a double column, and the six-way valve is switched to 1→6 (such as figure 1 D), at this time, monitor the composition after 35min, sample is injected once according to connection mode 1 and connection mode 2; mobile phase A is 0.2% phosphoric acid aqueou...

Embodiment 2

[0080] Embodiment 2: methodological investigation

[0081] The methodological inspection mainly includes stability, repeatability and precision. The inspection methods and results are as follows:

[0082] ①Stability test Take the same batch of sweet dream oral liquid samples, after pretreatment, under the optimized chromatographic conditions, adopt the column switching technology, inject samples at 0h, 2h, 4h, 8h, 12h and 24h respectively, record the chromatogram The results are shown in Table 3. It can be seen from Table 3 that with peak No. 20 as the reference peak, the relative retention time of the main chromatographic peaks and the relative peak area ratio have no obvious change, and the corresponding RSD values ​​are 0.04%-0.75% and 1.0%-4.8%, respectively, indicating that for The composition of the test solution is stable within 24h.

[0083] ②Precision test The same batch of Tianmeng Oral Liquid samples were taken. After pretreatment, under optimized chromatographic ...

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Abstract

The invention discloses a construction method and application of a fingerprint of sweet dream oral liquid. The method comprises the following steps: preparing a test solution, preparing a reference solution, optimizing and determining chromatographic conditions, measuring the fingerprint and processing the obtained fingerprint by using fingerprint software. Chromatographic columns with different stationary phases are connected through a six-way valve; gradient elution is performed by the same mobile phase; and various characteristic index components are well separated. Compared with an existing established fingerprint method, the method achieves that characteristic components of the sweet dream oral liquid can be separated and detected as many as possible, obtains more fingerprint characteristic peaks, and more comprehensively reflects component information of the oral liquid. The method further has the advantages of being easy and convenient to operate, high in precision, good in stability and reproducibility and the like, and the quality of the sweet dream oral liquid can be evaluated more comprehensively and scientifically, so that the quality and the curative effect of the product are guaranteed, and reference is provided for perfection of the quality standard of the product.

Description

technical field [0001] The invention belongs to the technical field of high performance liquid chromatography and quality control of traditional Chinese medicine preparations, and in particular relates to a method for constructing a fingerprint of Tianmeng oral liquid and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Tianmeng Oral Liquid is composed of Acanthopanax, Polygonatum, Silkworm Moth, Mulberry, Codonopsis, Astragalus, Amomum, Lycii, Hawthorn, Rehmannia glutinosa, Sunburned Epimedium, Tangerine Peel, Poria, Nuxychia, Pinellia, Ze A total of 17 medicinal materials of diarrhea and yam are refined by modern technology. It is a compound preparation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/02G01N30/34G01N30/20G01N30/46G01N30/60G01N30/74
CPCG01N30/02G01N30/20G01N30/34G01N30/46G01N30/6039G01N30/74G01N30/8634G01N30/8637G01N30/8679G01N30/8686G01N2030/201
Inventor 聂磊刘肖雁臧恒昌张惠苏美孙越姜红楚广诣
Owner SHANDONG UNIV
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