Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for identifying metabolites of phoxim in different tissues of freshwater fish

A technology for metabolites, phoxim, is applied in the field of aquaculture technology and detection and analysis to achieve the effects of expanding peak capacity, improving sensitivity and reliability, and reducing co-elution phenomenon.

Active Publication Date: 2022-02-18
YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even if the high-resolution (0.1u) tandem quadrupole mass spectrometer has a resolution of 1000 at a low mass-to-nucleus ratio (m / z 100) and a resolution of 10,000 at a high mass-to-nucleus ratio (m / z 1000) (FWHM) The measured mass-to-nucleus ratio (m / z) of the target can only be accurate to 1 decimal place

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for identifying metabolites of phoxim in different tissues of freshwater fish
  • A method for identifying metabolites of phoxim in different tissues of freshwater fish
  • A method for identifying metabolites of phoxim in different tissues of freshwater fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] 1 Test materials and methods

[0045] 1.1 Test drugs, test fish and breeding conditions

[0046] Phoxim EC (content ≥ 98%, provided by Xingtai Pesticide Co., Ltd.), acetone (excellent grade, Sinopharm Chemical Reagent Co., Ltd.), phoxim reference substance (content ≥ 98%, Dr.Ehrenstorfer Company, Germany, CAS No.: 14816-18-3), O, O, O', O',-tetraethyl phosphoryl dithioate (content ≥ 94.3%, Advanced ChemTech, USA, CAS No.: 3689-24-5), O, O-diethylthiophosphoric acid (content ≥ 98.0%, American Advanced ChemTech Company, CAS No.: 5871017-0), octinoxon (content ≥ 96.7%, German Dr. Ehrenstorfer Company, CAS No. : 14816-17 -2).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying metabolites of phoxim in different tissues of freshwater fish. The drug is administered by soaking, and qualitatively detected by ultra-high performance liquid chromatography in series with quadrupole time-of-flight high-resolution mass spectrometry (UPLC‑QTOFMS). Metabolites of phoxim in different tissues, including the following steps: (1) Freshwater fish were soaked in phoxim solution to collect different tissues; (2) Extract samples, the extraction agent was acetonitrile and ethyl acetate at a volume ratio of 5: 1 mixed, the purification agent is mixed with anhydrous magnesium sulfate and octadecylsilane bonded silica gel at a mass ratio of 10:1; (3) UPLC‑QTOFMS qualitative analysis: with phoxim, O, O, O ’ ,O ’ ,-Tetraethyl phosphorodithioate and O,O-diethyl phosphorothioate retention time and ion mass were compared to determine the metabolites of each tissue sample.

Description

technical field [0001] The invention belongs to the field of aquaculture technology and detection and analysis technology, and specifically relates to a method for qualitatively analyzing phoxim and its metabolites in freshwater fish tissues based on ultra-high performance liquid chromatography in series quadrupole time-of-flight high-resolution mass spectrometry. Background technique [0002] Phoxim is an organophosphate insecticide currently used as a veterinary drug to control mites, lice and other ectoparasites in pigs and sheep. In my country, phoxim has also been approved for killing or repelling Chinese bream, tilapia, grass carp, herring, silver carp, bighead carp, carp, crucian carp and other freshwater fish such as Chinese bream, anchor head bream, Fish lice, third-generation worms, ringworms, nematodes and other parasites. If phoxim is used improperly, it may cause its residues in aquatic animal tissues, thereby endangering human health. [0003] The main degrada...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/60G01N30/72G01N30/86
Inventor 刘永涛艾晓辉董靖王桢月胥宁杨秋红杨移斌周顺
Owner YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com