The invention discloses a method for improving the banana immature male flower embryonic
callus induction success rate, and relates to a method for establishing a banana embryonic suspension
cell system. The method comprises the following steps of: A, selecting a banana variety which is widely popularized at present or a banana variety which hardly induces embryonic
callus; B, selecting banana flower buds which bloom for 1-10 weeks, and preserving the flower buds for 24 hours before immature male flowers are officially cultivated; C, carrying out embryonic
callus induction; D, obtaining embryonic suspension cells; E, developing young embryos; and F. regenerating the young embryos into seedlings. According to the method, the purpose of improving the induction success rate of the banana immature male flower embryonic callus and the establishment success rate of the banana embryonic suspension
cell system is achieved by using different culture medium formulae and culture processes at different key stages of inducing the banana immature male flower embryonic callus and establishing the banana embryonic suspension
cell system; due to the application of the method, embryonic suspension cell systems of a plurality of triploid banana varieties which are widely popularized are effectively established, and the development of transgenosis of different banana varieties is facilitated.