The invention discloses a traditional Chinese medicinal composition and a capsule for invigorating qi and tonifying kidney and a preparation method of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition for invigorating qi and tonifying kidney is prepared by extracting various raw medicinal materials including herba epimedii, semen trigonellae, radix morindae officinalis, fructus psoraleae, radix polygalae, spina date seeds, poria cocos, radix angelica sinensis, arillus longan, radix astragali, white atractylodes rhizome, baked ginger, fructus ziziphi jujubae, licorice root, radix aucklandiae, canine kidney, radix ginseng rubra, colla cornus cervi, tortoise-shell glue and cortex cinnamomi by adopting water and alcohol. According to the technical scheme, the prescription of the traditional Chinese medicinal composition for invigorating qi and tonifying kidney is optimized, the traditional Chinese medicinal composition is prepared by selecting various traditional Chinese medicinal materials and adopting a suitable method, and the traditional Chinese medicinal composition can be used for warming and invigorating kidney yang, nourishing the liver and the kidney, reinforcing essence and blood, and strengthening muscles and bones, has the obvious efficacies of tonifying Yang, improving sexual capacity, resisting fatigue, resisting ageing, andenhancing immunity, and has the obvious curative effects for the symptoms of kidney-yang deficiency.