The invention discloses a Raman spectrum based substance qualitative detection method, and relates to a substance qualitative detection method. The method comprises the following steps of S1, establishing a Raman normalized second-order spectrum
library of a standard substance; S2, acquiring a
mask threshold; S3, respectively establishing a
spectrogram mask for each standard substance according to the Raman normalized second-order spectrum
library of the standard substance and the
mask threshold; and S4, judging the composition of the to-be-detected substance according to the
spectrogram mask of the standard substance. The qualitative Raman spectrum
library is established by using the spectrum second derivative-mask technology, the interference of
fluorescence on judgement is eliminated through the
processing of a second derivative, and the problem of Raman qualitativeness under the high
fluorescence background is solved; and besides, the second derivative can effectively reduce the width of the characteristic peak, and the identification accuracy is improved.
Database management and retrieval based on the spectrum second derivative-mask technology are more accurate, and the retrieval efficiency of the spectrum library is effectively improved.