The invention discloses an acridine-1, 3, 4-thiadiazole type compound and a preparation method and application thereof. The preparation method of the acridine-1, 3, 4-thiadiazole type compound comprises the following steps: 1) reacting by taking o-bromobenzoic acid and p-methyl aniline as raw materials, taking potassium carbonate and copper powder as catalysts and taking isoamyl alcohol or n-amyl alcohol as a solvent to obtain a compound 1; 2) cyclizing the compound 1 with phosphorus oxychloride to prepare a compound 2; 3) dissolving the compound 2 in an organic solvent, and then reacting with sodium thiocyanate in the presence of tetrabutyl ammonium bromide to prepare a compound 3; 4) taking the compound 3, dissolving in the organic solvent, and reacting with p-methoxybenzhydrazide to prepare a compound 4; and 5) taking the compound 4, reacting with concentrated sulfuric acid or acetic acid, adding water into a reactant to precipitate solids, and performing suction filtration for preparation. In-vitro anti-tumor test results show that the acridine-1, 3, 4-thiadiazole type compound has significant in-vitro anti-tumor activity against four subjects, namely MGC80-3, BEL-7404, NCI-H460 and T24.