The invention discloses a cell preserving fluid and a preparation method thereof. The cell preserving fluid comprises glucose, vitamins, amino acids, sodium chloride, methanol, albumin, phosphate, glycerin, heparin, hyaluronic acid and additives. The mass parts of the added glucose, the vitamin, the amino acid, the sodium chloride, the methanol, the albumin, the phosphate, the glycerol, the heparin, the hyaluronic acid and the additive are respectively 15% of glucose, 2% of vitamin, 3% of amino acid, 60% of sodium chloride, 5% of methanol, 3% of albumin, 3% of phosphate, 3% of glycerol, 3% ofheparin, 3% of hyaluronic acid and 1% of additive; the preparation method comprises the following steps: weighing glucose, vitamins, amino acids, sodium chloride, methanol, albumin, phosphate, glycerol, heparin, hyaluronic acid and additives in parts by mass, adding into a beaker, stirring and mixing on a magnetic stirrer for 30 minutes, and sterilizing the stirred mixed solution under an ultraviolet lamp for 15 minutes to obtain the cell preservation fluid. According to the invention, cells can be preserved for a long time, and the survival rate is high.