Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell preserving fluid and preparation method

A preservation solution and cell technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of DNA degradation and ingredients, and achieve the effect of low cell damage rate

Inactive Publication Date: 2020-06-05
深圳市天大生物医疗器械有限公司
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Oral exfoliated cell samples are an ideal way to obtain DNA, but inappropriate preservation solutions can cause DNA degradation or contain certain components that affect downstream genetic testing

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] Below, in conjunction with specific embodiment, the present invention is further described:

[0015] A cell preservation solution and a preparation method according to an embodiment of the present invention, comprising glucose, vitamin B 12 , Vitamin B 6 , amino acid, sodium chloride, methanol, albumin, phosphate, glycerin, heparin, hyaluronic acid and antioxidants, glucose, vitamin B 12 , Vitamin B 6 , amino acid, sodium chloride, methanol, albumin, phosphate, glycerin, heparin, hyaluronic acid and antioxidants are respectively glucose 15%, vitamin B 12 , Vitamin B 6 , amino acid 3%, sodium chloride 60%, methanol 5%, albumin 3%, phosphate 3%, glycerin 3%, heparin 3%, hyaluronic acid 3% and antioxidant 1%.

[0016] A preparation method of cell preservation solution, its specific preparation method is as follows:

[0017] (1) Weigh glucose, vitamins, amino acids, sodium chloride, methanol, albumin, phosphate, glycerin, heparin, hyaluronic acid and additives accordin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cell preserving fluid and a preparation method thereof. The cell preserving fluid comprises glucose, vitamins, amino acids, sodium chloride, methanol, albumin, phosphate, glycerin, heparin, hyaluronic acid and additives. The mass parts of the added glucose, the vitamin, the amino acid, the sodium chloride, the methanol, the albumin, the phosphate, the glycerol, the heparin, the hyaluronic acid and the additive are respectively 15% of glucose, 2% of vitamin, 3% of amino acid, 60% of sodium chloride, 5% of methanol, 3% of albumin, 3% of phosphate, 3% of glycerol, 3% ofheparin, 3% of hyaluronic acid and 1% of additive; the preparation method comprises the following steps: weighing glucose, vitamins, amino acids, sodium chloride, methanol, albumin, phosphate, glycerol, heparin, hyaluronic acid and additives in parts by mass, adding into a beaker, stirring and mixing on a magnetic stirrer for 30 minutes, and sterilizing the stirred mixed solution under an ultraviolet lamp for 15 minutes to obtain the cell preservation fluid. According to the invention, cells can be preserved for a long time, and the survival rate is high.

Description

technical field [0001] The invention relates to the technical field of cell storage, in particular to a cell storage solution and a preparation method. Background technique [0002] Cells are the basic unit of the structure and function of organisms, and the specificity of cells determines the specificity of individuals. Therefore, in-depth research on cells is the key to uncovering the mysteries of life, transforming life and conquering diseases. Cell biology has become the fastest-growing cutting-edge subject in contemporary biological sciences, and it is a compulsory course for biology, agronomy, medicine, animal husbandry, aquatic products and many biology-related majors. Since the 1950s, most of the Nobel Prizes in Physiology and Medicine have been awarded to scientists engaged in cell biology research. [0003] Compared with the traditional way of drawing blood to collect DNA, the DNA sample collection of oral exfoliated cells is a method of obtaining DNA that is harm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 曾亚平赖楚明
Owner 深圳市天大生物医疗器械有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com