The invention is a
cell aggregation device comprising a hydrogel substrate having at least one, preferably a plurality, of
cell-repellant compartments recessed into the uppermost surface. Each compartment is composed of an upper
cell suspension seeding chamber having an open uppermost portion and a bottom portion, and one, or more than one, lower
cell aggregation recess connected to the bottom portion of the upper cell suspension seeding chamber by a port. The
diameter of the port may be fully contiguous with the walls of the chambers and walls of the recesses, or the
diameter of the port may be more narrow than the walls of the chamber but fully contiguous with the walls of the recesses or more narrow than both the walls of the chamber and the walls of the recesses. The upper cell suspension seeding chambers are formed and positioned to funnel the cells into the lower
cell aggregation recesses through
gravitational force. The aggregation recesses are formed and positioned to promote cellular aggregation by coalescing cells into a finite region of minimum
gravitational energy, increasing intercellular contact and minimizing or preventing cell adherence to the substrate. A device for encapsulating aggregates of live cells is provided. The device comprises (i) a biocompatible, bio-sustainable substrate having a cell-encapsulating face composed of one or more biocompatible, bio-sustainable, spaced-apart, cell-encapsulating compartments extending therefrom and (ii) a
coating layer composed of a biocompatible, bio-sustainable
polymer that completely surrounds the substrate and the cell-encapsulating compartments. A method for making the device is also provided.