41results about How to "Differentiate" patented technology

A method of detecting and isolating cells that produce any secreted protein of interest (POI), comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.

A touch system comprises a touch surface and at least one camera acquiring images of the touch surface. A pointer contact data generator generates pointer position data in response to pointer contact with the touch surface, the pointer position data representing where on the touch surface pointer contact is made. A processor communicates with the at least one camera and the pointer contact data generator. The processor analyzes acquired images to determine the type of pointer used to contact the touch surface, and processes the pointer position data in accordance with the determined type of pointer. In the preferred embodiment the processor distinguishes between pointer tip touch surface contacts, pointer backend touch surface contacts and finger touch surface contacts. A writing function is invoked in response to pointer tip touch surface contacts. An erase function is invoked in response to pointer backend touch surface contacts. Mouse events are generated in response to finger touch surface contacts.

A material for making a conductive pattern, the material comprising a support and a heat-differentiable element, the heat-differentiable element comprising an outermost layer containing a polyanion and an intrinsically conductive polymer and optionally a second layer contiguous with the outermost layer, characterized in that the outermost layer and / or the optional second layer contains hydrophobic thermocoagulable latex particles in a weight ratio range with respect to the intrinsically conductive polymer in the range of 20:1 to 1:5, the hydrophobic thermocoagulable latex particles are capable upon heating of increasing the conductivity of the heated parts of the outermost layer relative to the unheated parts of the outermost layer and / or changing the removability of the heated parts of the outermost layer relative to the unheated parts of the outermost layer and the heat-differentiable element does not comprise a di- or polyhydroxy organic compound or an aprotic compound with a dielectric constant, ∈, ≧15; a method of making a conductive pattern on a support therewith; and a use of the material for making a conductive pattern in making an electronic circuit in the production of an electric or semiconductor device such as a printed circuit board, an integrated circuit, a display or touch screen, an electroluminescent device or a photovoltaic cell.

A method of detecting and isolating cells that produce a secreted protein of interest (POI) that has a T cell receptor variable domain, comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.

The instant application relates to methods and reagents for modulating the Hedgehog signaling pathway using RNA interference technology (RNAi). The application provides potential targets of the Hedgehog RNAi, methods to identify additional Hedgehog signaling pathway components, methods to inhibit Hedgehog signaling targets using siRNA, and their uses in the treatment of a number of disease conditions.

Recombinant cell surface capture proteins and detection molecules that are useful for isolating and detecting cells that produce a secreted heterodimeric protein of interest (POI) that has an immunoglobulin CH3 domain and / or substituted CH3 domain are provided. Recombinant cell surface capture proteins and detection molecules that isolate and detect bispecific antibodies are also provided. The invention also provides recombinant antigen-binding proteins that are capable of recognizing and binding to proteins of interest that contain a CH3 domain and / or a modified CH3 domain, such as a CH3 domain with or without amino acid substitutions at H95 and Y96 (IMGT).

A method of detecting and isolating cells that produce a secreted protein of interest (POI) that has an immunoglobulin CH3 domain and / or substituted CH3 domain, comprising: a) constructing a cell line transiently or stably expressing a cell surface capture molecule, which binds the POI, by transfecting the cell line with a nucleic acid that encodes such cell surface capture molecule; b) transfecting said cell simultaneously or subsequently with a second nucleic acid that encodes a POI wherein such POI is secreted; c) detecting the surface-displayed POI by contacting the cells with a detection molecule, which binds the POI; and d) isolating cells based on the detection molecule.

The present invention relates to use of miR-18b for prevention, treatment, or diagnosis of muscle diseases or neuromuscular diseases, and specifically it was confirmed that, in a muscle disease by gene mutations model, gene mutations reduce miR-18b expression, cause dysregulation of miR-18b signaling pathways, and thus induce calcium signaling, cell differentiation inhibition, and apoptosis. Therefore, miR-18b of the present invention may be used as a target factor for diagnosing and treating muscle diseases caused by gene mutations such as ALS and DMD.

The invention discloses a multi-means and low-cost fan fault monitoring method, which includes the following steps: (1) defining a fan fault state set according to the common faults of fans in practical application; (2) optimizing a fault feature set by using a common fan fault feature set template and the existing fault data of a target fan; (3) continuously collecting the instantaneous current I, surface temperature T and vibration L signals of the fan, and calculating the feature values of the current, temperature and vibration signal of the fan at the current time; and (4) matching the calculated feature values of the current, temperature and vibration signal of the fan with the items in the fan fault feature set, and judging that the current fault state of the fan is the fault state represented by the matching item in the fault state set if the calculated feature values of the current, temperature and vibration signal of the fan are consistent with the items in the fan fault feature set. By monitoring and analyzing the current, temperature and vibration signals of fans, timely and accurate monitoring of fan faults is realized. The multi-means and low-cost fan fault monitoringmethod has the characteristics such as low cost, easy integration and low difficulty in use.

The invention discloses a method for judging a hinge producing annulations of a parallel machine tool. The annulations exist on the surface of a workpiece machined by the parallel machine tool, the corresponding relation of the position of annulations on the surface of the workpiece and the hinge producing the annulations when a connecting rod reversely rotates around the hinge axis of a universal hinge relative to a hinge hole, and on the basis and according to the corresponding relation of the position of annulations on the surface of the workpiece and the hinge producing the annulations when the connecting rod reversely rotates around the hinge axis of the universal hinge relative to the hinge hole, the hinge producing the annulations of the parallel machine tool is judged, and the workpiece of the hinge producing the annulations of the parallel machine tool is a one-layer or multiple-layer cylindrical workpiece machined by the parallel machine tool. The method is used for judging the hinge producing the annulations of the parallel machine tool and performing hinge maintenance, gap adjustment or re-design and replacement service and improves the machining accuracy of the parallel machine tool.

The present invention relates to a method of modulating differentiation, adhesion and / or survival of the neural cell adhesion molecule (NCAM) presenting cells by providing compounds capable of modulating the interaction between the Ig1, Ig2 and / or Ig3 modules of NCAM. The invention provides candidate compounds capable of modulating the interaction between the Ig1, Ig2 and / or Ig3 modules of NCAM by using methods for screening and testing described in the application. The invention further relates to pharmaceutical compositions comprising compounds capable of modulating the interaction between the Ig1, Ig2 and / or Ig3 modules of NCAM and to use of the pharmaceutical compositions and compounds for the modulation of differentiation, adhesion and / or survival of NCAM presenting cells.

The invention discloses a vehicle-mounted forewarning terminal for driving behaviors based on information fusion; the vehicle-mounted forewarning terminal comprises a face image acquisition and analysis unit, a driving operation information acquisition and analysis unit, an information fusion analyzer, a forewarning judging controller, a car-interior forewarning unit and a remote alarming unit, wherein the face image acquisition and analysis unit and the driving operation information acquisition and analysis unit are respectively communicated with the information fusion analyzer; the information fusion analyzer is communicated with the forewarning judging controller which is respectively communicated with the car-interior forewarning unit and the remote alarming unit. The vehicle-mounted forewarning terminal for driving behaviors based on information fusion is applied to monitoring, analyzing and forewarning dangerous driving behaviors, is good in compatibility and strong in expandability and can be conveniently integrated with other vehicle information recording systems and vehicle monitoring systems.

The invention discloses a method for discriminating the validity of interval five defense data applicable to substations, comprising the following steps: step SS1: reading the five defense logic expressions; step SS2: extracting each logic in the five defense logic expressions variable; step SS3: determine the corresponding data quality of the logical variable is Quality; step SS4: judge the validity of logical variable data one by one according to the value of the data quality bit Quality of the logical variable. The present invention can accurately, comprehensively and reliably judge the validity of the interval five-defense data by analyzing and processing the interval five-defense data of the substation control layer, making the judgment of the five-defense expression more reasonable, avoiding misoperation, and providing on-site operation and maintenance personnel with security.

The invention discloses a vertical-shaft wind turbine rotary spindle vibration restraining method which is high in wind energy capturing efficiency. The method includes: acquiring vertical inclination angle of a rotary spindle in real time; calculating vibration intensity of two orthogonal directions in a horizontal section of the rotary spindle and optimal rotation speed of a wind turbine generator under a maximum tracking target; determining expected rotation speed; allowing a rotating speed inner ring controller to drive the rotation speed to reach the expected rotation speed fast; and starting a reverse electric braking function of the rotating speed inner ring controller and a electromagnetic braking mechanism of the rotary spindle when vibration intensity of the spindle exceeds a preset safety regulating threshold; wherein fast rotating speed regulating of the rotating speed inner ring controller is achieved by motor acceleration through directly cutting of part of phase winding excitations or motor deceleration through releasing current chopping limits. By the method, current vibration intensity of the spindle can be calculated in real time, spindle vibration can be restrained, and the wind turbine generator is enabled to acquire the highest wind energy capturing efficiency as far as possible while mechanical stability is maintained.