43 results about "Streptomyces aureus" patented technology

The invention discloses a keratin producing bacterial strain as well as culture conditions and crude enzyme property thereof and belongs to the technical field of microorganisms. Through identification, the bacterial strain is Streptomyces aureofaciens, named as K13 and preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number of CGMCC NO.8047. Through preliminary optimization, the culture medium of the bacterial strain comprises 0.5-1% of wool, 4-8% of glucose, 3-5% of peptone, 0.3% of sodium nitrate, 0.04% of dipotassium phosphate and 0.05% of sodium chloride; the shake culture conditions are that the initial pH (potential of Hydrogen) value is 10.0, the inoculation quantity is 10%, the culture temperature is 30 DEG C, the rotating speed is 220 revolutions per minute, the liquid loading volume is 30ml / 250ml, the fermentation period is 64 hours and the keratinase producing activity is up to 44.2U / ml. The keratinase is mesophilic alkaline keratinase, the optimum operation temperature is 50-60 DEG C and the optimum pH is 8.5; the enzyme activity of the keratinase is stable in an alkaline environment (pH 7.0-10.0).

The invention relates to a seed culture medium and a culture method of Streptomyces aureus. The seed culture medium contains brewer's yeast dry residue, which is composed of 8-10ml / L of oil, 20-22g / L of starch, and 27-29g / L of brewer's yeast dry residue. L. Yeast extract 8-9g / L, low-temperature soybean cake powder 11-13g / L, dipotassium hydrogen phosphate 0.3-0.4g / L, light calcium carbonate 0.8-1g / L, magnesium sulfate 0.04-0.06g / L, Sodium chloride 0.8-0.9g / L, amylase 0.2-0.3g / L, ammonium sulfate 0.05-0.07g / L and polyether defoamer 0.03-0.04g / L. The present invention can effectively improve the concentration of Streptomyces aureus cells and the quality of cell culture, reduce the inoculum amount at the initial stage of seed culture, shorten the seed culture period, and at the same time reduce the amount of raw and auxiliary materials in seed culture to the greatest extent, reduce production costs, and ensure sufficient supply. Achieve stable and high-quality cultivation of Streptomyces aureus.

The invention discloses a preparation method for tetracycline. The preparation method comprises the following specific steps of preparing a streptomyces aureus strain and then performing seed shake-flask culture and seed tank culture; when culture is performed for 20-26 hours, converting the culture mode into fermentation culture; when the pH value reaches 5.6-7.6, the total titer is not smaller than 8,000U / ml, the total saccharides content is not greater than 2.0 percent, the quantity of amino nitrogen is greater than 50mg / 100mL, and the filtering velocity is not smaller than 5mL / 5min, ending the fermentation and discharging from a tank; finally, filtering with a plate frame, decoloring ion exchange resin, crystallizing and performing air current drying to obtain the tetracycline. According to the preparation method disclosed by the invention, a fermentation process is simple, the fermentation unit can be effectively improved, the yield is improved, and the production cost is reduced; meanwhile, the use of chemical organic solvent is avoided.

The invention discloses a wettable powder of Streptomyces aureus verticalus HN6, which is prepared from the following components in mass percentage: 5%-20% of liquid fermentation product of Streptomyces aureus verticalis HN6, 1%-5% of wetting agent, dispersing 1% to 5% of the agent, 0.5% to 2% of the stabilizer, and 100% of the filler. The preparation method includes: putting wetting agent, dispersant, stabilizer and filler into the airflow mill according to the above weight to pulverize, passing through a 325-mesh sieve, and after cooling down, adding the liquid fermentation product of Streptomyces aureus verticalus HN6, and continuing pulverization, the moisture content is ≤ 5%, the number of active spores is 0.1 to 1 billion / g, which is qualified after inspection and packed for storage. Beneficial effects of the present invention: the medicament belongs to living microorganism preparation, has remarkable effect on banana wilt, has good control effect on other diseases, has no toxic and side effects on ecological environment, and can guarantee the quality and safety of agricultural products.

The invention discloses a strain of Streptomyces albus Z9 for preventing and treating sclerotinia in sunflower seedling stage. The preservation number of the strain is CGMCC 13115, which belongs to the technical field of microorganisms. Through the implementation of experiments such as the isolation, activation and identification of the pathogenic sclerotinia of sunflower sclerotinia, the primary screening and re-screening experiments of antagonistic bacteria, and the identification and analysis of antagonistic bacteria, it was shown that the strain Z9 has a strong ability to inhibit the hyphae of Sclerotinia sclerotiorum ability to grow. The infection rate of sunflower seed husks treated by the strain Z9 fermented original bacterial liquid of the present invention is reduced by 70%, the germination promotion rate of the bacterial strain Z9 fermentation filtrate is 33.3%, and the control effect of the isolated leaves of the bacterial strain Z9 fermented original bacterial liquid 69.6%, and the pot experiment control effect of bacterial strain Z9 fermented original bacterium solution is 66.7%, which can effectively inhibit the growth and reproduction of Sclerotinia sclerotiorum. The bacterial strain of the invention has simple culture conditions, is easy to preserve, is environmentally friendly, and has good development and application value.

The invention relates to a method for producing tetracycline by fermentation of streptomyces aureus. The method comprises the following steps: slant spore culture, seed culture and fermentation culture of the streptomyces aureus, and is characterized in that dextrin, sucrose, corn pulp, hydrolyzed soybean meal and other medium-effect carbon-nitrogen sources are adopted to replace original late-effect carbon-nitrogen sources; by reducing the contents of starch and bean flour in an original fermentation culture medium and combining with a formula of a mixture in the fermentation process and adjustment of a replenishing method and placing part of the nitrogen sources in the fermentation culture medium into material replenishment in the fermentation process, the concentration of the culture medium in the fermentation process is effectively controlled, the oxygen dissolution condition in the early stage of fermentation is improved, the final residue of the culture medium of fermentation is controlled by material replenishment, the material consumption is reduced, and the emission of solid wastes is reduced; the metabolism velocity in exponential growth phase in the fermentation process of the tetracycline is effectively controlled, the abnormal situations that a fermentation solution turns red and becomes sour and the like in the fermentation process are reduced, and the fermentation level is improved.

The invention discloses an organic microbial fertilizer for lettuces and a preparation method thereof. The organic microbial fertilizer for lettuces is prepared from the following components in parts by weight: 4-7 parts of streptomyces aureus, 0.6-0.9 part of bacillus thuringiensis, 3-5 parts of enzyme bacteria, 900-1100 parts of waste liquid precipitates, 240-380 parts of an inorganic fertilizer, 45-55 parts of trace elements, 25-32 parts of an adhesive, 0.5-0.7 part of a stabilizer and 0.1-0.3 part of an effect-stabilizer assistant. The organic microbial fertilizer prepared by the method is capable of increasing the yield of the lettuce, the content of vitamin C and the content of an iron element; and the content of nitrate is reduced, so that the quality of the lettuce is enhanced greatly. Compared with other fertilizers containing the same nutrients, the organic microbial fertilizer is capable of decreasing or lowering crop disease-pest damages which affect the crop quality. The using quantity of a chemical fertilizer is not decreased, and the organic microbial fertilizer is beneficial to environmental protection.