A method for identifying rice blast resistance of rice comprises the steps of scissoring three rice stems in a large
bract period from a rice growing field, bringing the rice stems back to a laboratory, scissoring 2cm from the upper portion of each stem section and 5-6cm from the lower portion of each stem section to obtain one rice stem with the whole length of approximate 7-8cm, putting the three rice stems into culture dishes with two
layers of
filter paper, adding
sterile water of 1mL into each culture dish, washing cultivated rice blast
fungus spores with the
sterile water, obtaining concentration of 106 pcs / ml, dropping
spore suspending liquid of 8 muL to stem section sites of each rice stem through a liquid moving gun, putting the culture dishes into a illuminating
incubator at the temperature of 26 DEG C, wrapping the culture dishes with transparent films to keep
humidity in the culture dishes, cultivating for ten days in light and dark alternating
modes to survey
disease conditions, dividing survey
disease levels into three levels, regarding the first level as
disease-resistant cultivars, regarding the second level as susceptible cultivars, and regarding the third level as highly susceptible cultivars. The method optimizes the concentration and
inoculation amount of inoculated rice blast
fungus spores and establishes simple, convenient and accurate grading standards.