The invention discloses an expression and purification method of chicken
growth hormone recombinant
protein in
Pichia pastoris. The expression and purification method includes the steps of firstly, extracting
total RNA (ribonucleic acid) from chicken pituitary, performing reverse transcription PCR (
polymerase chain reaction) to obtain cDNA (
complementary deoxyribonucleic acid) and conducting PCR by taking the cDNA as a template so as to obtain chicken
growth hormone gene fragments; secondly, using the
Pichia pastoris for constructing a
recombinant expression vector pPIC9K-cGH; thirdly, subjecting
Pichia pastoris GS115 to electrotransformation by the
recombinant expression vector pPIC9K-cGH and screening to obtain a transgenic
recombinant yeast strain GS115-pPIC9K-cGH; fourthly, obtaining the chicken
growth hormone recombinant
protein through
methanol induced expression and purification, and identifying activity of the chicken growth
hormone recombinant
protein through
cell activity test. In the expression and purification method of the chicken growth
hormone recombinant protein in the
Pichia pastoris, high-efficiency expression of the cGH (chicken growth
hormone) recombinant protein in the
Pichia pastoris is achieved and a purification
system is established firstly, so that the high-purity chicken growth hormone recombinant protein with activity can be obtained. The expression and purification method of the chicken growth hormone recombinant protein in the
Pichia pastoris is simple and convenient to operate, high in yield and low in cost and provides a basis for further studies on structures, functions and production application of chicken growth hormone.