32 results about "Phalaenopsis amabilis" patented technology

The invention discloses a method for cultivating moth orchid in a solar greenhouse in the north, and belongs to a flower and plant cultivation method. A method for constructing the solar greenhouse comprises the steps of mounting round-wing heating pipes around the greenhouse, building a seedling bed, mounting a second curtain for the seeding bed in winter and covering a shading net outside the greenhouse in spring, summer and autumn. The method for cultivating moth orchid comprises the steps of selecting seeds, cultivating seedlings in test tubes, changing pots, watering and applying fertilizer, spraying agricultural chemical, arranging seedlings and seedling plates, cultivation temperature, light control standards, water and humidity control standards, fertilizer use standards N-P-N, flowering period regulation, supporting stalks, sorting finished flowers and packing and transporting finished flowers. The method cultivates moth orchid with exuberant branches and leaves and buds through improvement of the solar greenhouse, regulation of cultivation environment and flowering period, control of diseases and insects and finds a new field for cultivating moth orchid in the north.

The invention provides a culture medium for phalaenopsis amabilis tissue culture. The culture medium comprises the following components: 1900 mg/L of KNO3; 1650 mg/L of ammonium nitrate; 170 mg/L of potassium dihydrogen phosphate; 370 mg/L of magnesium sulfate heptahydrate; 440 mg/L of calcium chloride dihydrate; 0.60-0.62 mg/L of KI; 4.5-4.8 mg/L of boric acid; 25.0-25.3 mg/L of manganese sulfate tetrahydrate; 7.0-7.2 mg/L of zinc sulfate heptahydrate; 0.20-0.22 mg/L of sodium molybdate dihydrate; 0.025 mg/L of copper sulfate pentahydrate; 0.025 mg/L of cobalt chloride hexahydrate; 37.25 mg/L of Na2.EDTA; 27.85 mg/L of ferrous sulfate heptahydrate; 100 mg/L of inositol; 2 mg/L of glycine; 0.1 mg/L of thiamine hydrochloride; 0.5 mg/L of pyridoxine hydrochloride; 0.5 mg/L of nicotinic acid; 20-22 g/L of sucrose; 5-5.2 g/L of agar; 30-35 g/L of a banana juice; 3-5 g/L of a pear juice; 0.45-0.48 g/L of activated carbon; and a solvent being deionized water. With application of the culture medium to culture phalaenopsis amabilis, the survival rate is more than 96%, the bacteria infection rate is 2.7-2.8%, and the browning degree of the culture medium is 30-40% lower than that of an MS culture medium.

The invention discloses a tissue culture method for phalaenopsis amabilis; in the steps, the inoculation density and the sowing density can produce a 'quorum sensing', so that the seed germination becomes faster; a sterile germination culture medium includes tryptone, a banana juice, active carbon and agar and the ratio and can provide nutrition needed for seed growth, and the germination of the seeds is promoted; hormones are added during germination, the adult seedling number can be allowed to be increased; an organic additive is added during germination, the organic additive is saccharose, the saccharose concentration is 2-4%, and the organic additive has a promoting effect on phalaenopsis amabilis seed germination, protocorm formation and leaf growth. The phalaenopsis amabilis cultured by the tissue culture method has the advantages of high germination rate, excellent growth, huge market potential and broad prospects.

The invention relates to the field of plant tissue culture, in particular to a phalaenopsis amabilis tissue culture propagation method. The phalaenopsis amabilis tissue culture propagation method comprises the following steps of induction of pedicel buds, differentiation and proliferation of cluster buds, shoot strengthening and root induction, seedling hardening and transplanting. An induction culture medium is prepared from mashed potatoes and mashed bananas, and saccharose is replaced by white granulated sugar, so that the production cost of large-scale proliferation of phalaenopsis amabilis can be greatly reduced, and the growth situation is good; coconut milk in a differentiation and proliferation culture medium is also one of key factors of proliferation, and the proliferation rate, the proliferation time and the economic benefit are the best when the content of the coconut milk is 10 percent. By adopting the phalaenopsis amabilis tissue culture propagation method of the technical scheme, tissue culture and rapid propagation are carried out along a path of the cluster buds of the phalaenopsis amabilis, the phalaenopsis amabilis tissue culture propagation method has the advantages of high inductivity, short induction time, high proliferation rate of the cluster buds, low technical difficulty and stable heritability, a large amount of variation can be prevented from happening during production, and a great significance in large-scale production is obtained.

The invention relates to a special soilless culture nutritional meal for butterfly orchid and preparation and application of the special soilless culture nutritional meal. The special soilless culture nutritional meal for the butterfly orchid consists of a nutritional matrix and a nutritional fertilizer in mass ratio of (970-985):(15-30), wherein the nutritional fertilizer consists of a nutritional fertilizer A, a nutritional fertilizer B and a nutritional fertilizer C in mass ratio of (1-2.5):(4.5-6):(2.5-3.5). The nutritional matrix comprises expanded perlite, expanded vermiculite, sawdust,calcium chloride, magnesium chloride and ferrous sulfate in raw material. The nutritional matrix, the nutritional fertilizer A, the nutritional fertilizer B and the nutritional fertilizer C are respectively and independently packaged. According to the special soilless culture nutritional meal for the butterfly orchid, the nutritional matrix compounded in consideration of absorption of nourishmentat different growth stages of the butterfly orchid is suitable for the well growth of the butterfly orchid in water-retaining property, nutrient preserving capability and vent ability, and meanwhile,the nutritional fertilizer is blended into an aqueous solution applied in the manner of watering, and through tests, a good effect is obtained.

The present invention relates to a method for cultivating phalaenopsis amabilis in a northern glasshouse. The method comprises the following steps: planting phalaenopsis amabilis seedlings in a matrix (a mixture of peat soil and perlite according to a volume ratio of 4:1 to 5:1); thoroughly watering the whole matrix with water having a pH value of 6.0-6.8; spraying the plant with a 800-time liquid of chlorothalonil wettable powder 2-4 days after watering; transferring the mature phalaenopsis amabilis seedlings into a greenhouse 4-5 months before expected flowering; keeping temperature of the greenhouse at 30-32 DEG C during daytime and at 24-26 DEG C during night; adjusting illumination in the greenhouse to 10000-12000 Lux; and applying fertilizers at regular intervals, wherein a balance fertilizer with a N:P:K ratio of 20:20:20 is applied every week along with water.

The invention provides a gene PeGRF6 for regulating and controlling development of butterfly orchid leaves and application of the gene PeGRF6. The invention firstly provides a gene PeGRF6 for regulating and controlling the development of butterfly orchid leaves. The gene PeGRF6 has one of the following nucleotide sequences: 1) a nucleotide sequence as shown in SEQ ID NO.1; 2) a nucleotide sequence which is derived by substitution, deletion or addition of one or more nucleotides of the nucleotide sequence as shown in SEQ ID NO.1; and 3) a nucleotide sequence having at least 80% homology with the SEQ ID NO.1. The gene provided by the invention can effectively regulate and control the development of the leaves of the Phalaenopsis amabilis, reduce the leaf area of new leaves of the Phalaenopsis amabilis, improve the content of anthocyanin and chlorophyll in the leaves, and provide a theoretical basis for breeding of the Phalaenopsis amabilis.

The invention discloses a method for preventing and controlling phalaenopsis amabilis brown spot. The method is characterized by comprising the following steps of: mixing turfy soil and perlite according to a volume ratio of 4:1-5:1 to form a culture substrate, wherein the temperature is between 25 and 28 DEG C in the daytime and is between 18 and 20 DEG C in the evening, the humidity is between 70 and 80 percent in the daytime and is between 75 and 85 percent in the evening, and the illumination is between 10,000 and 12,000Lux; spraying solution formed by dissolving thiophanate methyl wettable powder in 800 to 1,000 times water once every month after seedlings are introduced; continuously spraying for 5 months; and irrigating with water or fertilizer in the following way during the spraying: irrigating with clear water when emission concentration (EC) value of soil is more than 0.4; and irrigating with aqueous solution which is formed by mixing 0.3 to 0.4g of peters product 20-10-200, 0.05 to 0.07g of peters product 15-20-25 and 0.16 to 0.18g of MgSO4 in every liter of water and aqueous solution which is formed by dissolving 0.8 to 0.9g of peters product 20-20-20 in every liter of water in turn when the EC value of the soil is less than 0.4.

The invention belongs to the technical field of monitoring of plant varieties and in particular discloses a method for detecting phalaenopsis amabilis mutants. Four varieties including 'HongMantianHong', 'Guangmangsishe', V31 and 'Hongzhenzhu' and mutants thereof can be completely distinguished through utilizing combination of ten pairs of primers including SSR1, SSR2, SSR3, SSR4, SSR5, SSR6, SSR7, SSR8, SSR9 and SSR10; sequences of the ten pairs of primers including the SSR1, the R2, the SSR3, the SSR4, the SSR5, the SSR6, the SSR7, the SSR8, the SSR9 and the SSR10 are shown as SEQ: 1 to 20. The detection method disclosed by the invention can be used for screening the mutants, which do not meet growth requirements, at a bottle seedling period, so that the production of large-scale production is saved.

The invention discloses a rapid propagation method of phalaenopsis amabilis flower stalk stem sections. The method comprises the following steps of (1) preparing a culture medium; (2) preparing explants; (3) performing inoculation; and (4) performing management after inoculation. The rapid propagation method of the phalaenopsis amabilis flower stalk stem sections provided by the invention has theadvantages that the browning and deterioration of callus tissues are effectively prevented; the bacterial infection rate of the tissue culture of phalaenopsis amabilis is also greatly reduced; the callus tissue division, differentiation and proliferation capacity is improved; the propagation rate of the tissue culture of the phalaenopsis amabilis is further improved; and a high market popularization value is realized.

The invention discloses a method for preparing a rapid reproduction culture medium for tissue culture of phalaenopsis amabilis. The method comprises the following steps: (1) performing fumigation treatment; (2) performing crushing treatment; (3) performing homogenization treatment; (4) weighing raw materials; and (5) preparing a finished product. By adopting the culture medium finally prepared bythe method, rich nutrient elements can be provided for splitting and differentiation of phalaenopsis amabilis tissue, callus browning deterioration can be effectively prevented, the contamination rateof phalaenopsis amabilis tissue culture can be also greatly reduced, the callus splitting and differentiation capability can be improved, furthermore, the breeding rate of phalaenopsis amabilis tissue culture can be increased, and very high market popularization value can be made.

The invention discloses a special phalaenopsis amabilis controlled release fertilizer produced by utilizing waste agaricus bisporus bracts and a preparation method. The controlled release fertilizer is mainly prepared from the following materials in parts by weight: 46-48 parts of waste agaricus bisporus bracts, 8-10 parts of perlite, 8-10 parts of coating agent, 1.6-1.7 parts of water-retaining agent, 13-15 parts of sawdust and 4-6 parts of slow release fertilizer. The controlled release fertilizer and the preparation method have the positive and beneficial effects that an approach for scientifically recycling the waste agaricus bisporus bracts is provided, thus promoting development of cyclic economy; the usage amount of common compound fertilizers can be effectively reduced by adopting the produced organic fertilizer; the obtained organic fertilizer has good application effects and has the effect of increasing the granular structure of soil, so that the soil is loosened and is valid especially for cultivation of phalaenopsis amabilis with aerial roots; and quick lime is used for regulating the pH value and killing harmful bacteria in the waste agaricus bisporus bracts, thus effectively preventing regeneration of harmful bacteria in the roots of phalaenopsis amabilis.