Method for quickly breeding new butterfly orchid variety
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A new variety, phalaenopsis technology, applied in the field of rapid breeding of new varieties of phalaenopsis, can solve the problem of long breeding cycle of phalaenopsis, and achieve the effect of shortening the breeding time and improving the breeding efficiency.
Active Publication Date: 2020-10-13
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology
At present, hybrid breeding is still the most effective method for breeding new varieties of Phalaenopsis, but the long breeding cycle of Phalaenopsis is the most prominent problem in Phalaenopsis breeding
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Embodiment 2
[0055] (1) Hybrid pollination: female parent is P316, male parent is P25
[0056] Carry out pollination hybridization in February-April, and pollinate 2-3 flowers to ensure the success rate. The temperature is controlled at 28±2°C, the humidity is controlled at 70-80%, and the fruit pods mature about 120 days after pollination.
[0057] (2) Aseptic sowing: Rinse the ripe fruit pods with tap water, soak them in 75% alcohol for 30 seconds on the ultra-clean workbench, and then soak them with 0.1% HgCl 2 The solution was sterilized for 8-10 minutes, rinsed with sterile water for 5-8 times, blotted the remaining water with sterile filter paper and cut the fruit pods, and spread the powdered seeds evenly in the germination medium, the germination medium was Hyponex (flower Bao No. 1) 3.0g / L+NAA (naphthaleneacetic acid) 0.5mg / L+10.0% coconut milk+sucrose 30g / L+agar 7g / L, pH value 5.5-5.8. The culture temperature is 25-28°C, dark culture in the early stage, light intensity 1500-200...
Embodiment 3
[0068] (1) Hybrid pollination: female parent is P25, male parent is HHS
[0069] Carry out pollination hybridization in February-April, and pollinate 2-3 flowers to ensure the success rate. The temperature is controlled at 28±2°C, the humidity is controlled at 70-80%, and the fruit pods mature about 120 days after pollination.
[0070] (2) Aseptic sowing: Rinse the ripe fruit pods with tap water, soak them in 75% alcohol for 30 seconds on the ultra-clean workbench, and then soak them with 0.1% HgCl 2 The solution was sterilized for 8-10 minutes, rinsed with sterile water for 5-8 times, blotted the remaining water with sterile filter paper and cut the fruit pods, and spread the powdered seeds evenly in the germination medium, the germination medium was Hyponex (flower Bao No. 1) 3.0g / L+NAA (naphthaleneacetic acid) 0.5mg / L+10.0% coconut milk+sucrose 30g / L+agar 7g / L, pH value 5.5-5.8. The culture temperature is 25-28°C, dark culture in the early stage, light intensity 1500-2000...
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Abstract
The invention provides a method for quickly breeding a new butterfly orchid variety. The method sequentially comprises the following two steps: cross pollination, sterile seeding, seed germination anddifferentiation culture. The differentiation stage is divided into two stages: (1) picking out 5-10 rootless seedlings, respectively and independently carrying out multiplication subculture, and carrying out rooting and transplantation culture to obtain meristematic seedlings; and (2) carrying out rooting culture on the remaining seedlings to obtain seedling plants. The meristematic seedlings andthe seedling plants are respectively subjected to seedling exercise, are taken out of a bottle, are transplanted, and finally forms flowering plants though planting small, medium and large seedlings.The method screens out high-quality strains through comparison, forms a new variety through testing and evaluation, screens out high-quality individuals which do not exist in the meristematic seedling flowering plants from the flowering plants of the seedling plants, and forms the new variety through single-plant cloning propagation, planting, testing and evaluation. According to the method, thenew variety breeding time is obviously shortened, the phalaenopsis amabilis breeding efficiency is remarkably improved, and complementary advantages are formed by combining a rapid breeding method anda traditional breeding method.
Description
technical field [0001] The invention belongs to the technical field of rapid propagation by tissue culture, and in particular relates to a method for rapid breeding of a new Phalaenopsis variety. Background technique [0002] Phalaenopsis is a Phalaenopsis (Orchidaceae) plant, most of which are distributed in hot and humid Asian regions, and artificially bred Phalaenopsis can be planted all over the world under greenhouse cultivation conditions, because of its unique flower shape, bright colors, Rich in color, neat inflorescences, and long flowering period, it has become a popular tropical orchid all over the world. It is deeply loved by consumers at home and abroad, and is known as the "Queen of Orchids". Through the efforts of orchid breeders and orchid lovers all over the world, as of May 22, 2019, 35,971 Phalaenopsis hybrid combinations have been officially registered with the Royal Horticultural Society (RHS). In the 1980s, the Taiwan region of our country established ...
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