Method for preparing rapid reproduction culture medium for tissue culture of phalaenopsis amabilis

A technology of Phalaenopsis and culture medium, which is applied in the field of preparation of Phalaenopsis tissue culture rapid propagation medium, can solve the problems of low bacterial infection rate, low browning rate, easy browning and deterioration of explants, etc., and reduces bacterial contamination. The effect of preventing browning and deterioration, and improving reproduction rate

Inactive Publication Date: 2020-06-19
安徽惠泽园苗木花卉种植有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many researches on the tissue culture of Phalaenopsis, there are still many problems in the tissue culture of Phalaenopsis that have not been improved. In the process of tissue culture, the explants are easy to brown and deteriorate and then die, and prevent the explants from Bacteria contamination is also a relatively common problem during the induction process, so it is particularly important to develop a medium with low bacterial contamination rate and low browning rate suitable for Phalaenopsis cultivation

Method used

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  • Method for preparing rapid reproduction culture medium for tissue culture of phalaenopsis amabilis

Examples

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Effect test

Embodiment 1

[0027] A preparation method of Phalaenopsis tissue culture rapid propagation medium, comprising the steps of:

[0028] (1) Fumigation treatment:

[0029] First weigh 13 parts of wax gourd rind, 8 parts of watermelon rind, 5 parts of amaranth stalk, 10 parts of cucumber rind, and 12 parts of pumpkin vine in corresponding weight parts and put them together on a wooden sieve, then steam fumigate under the wooden sieve, and after 30 minutes of fumigation , take out the mixed raw materials for later use;

[0030] (2) Crushing treatment:

[0031] Put the mixed raw materials after the fumigation treatment in step (1) into a cryogenic pulverizer for pulverization treatment, after pulverization treatment for 23 minutes, pass through a 40-mesh sieve for later use;

[0032] (3) Homogenization treatment:

[0033] Put the sieved powder and sterile water in step (2) into the micro-jet high-pressure homogenizer according to the weight-to-volume ratio of 1g:4mL, and then perform homogeniza...

Embodiment 2

[0046] A preparation method of Phalaenopsis tissue culture rapid propagation medium, comprising the steps of:

[0047] (1) Fumigation treatment:

[0048] First weigh 14 parts of wax gourd rind, 9 parts of watermelon rind, 6 parts of amaranth stalk, 11.5 parts of cucumber rind, and 13.5 parts of pumpkin vine in corresponding weight parts and place them on a wooden sieve, and then steam fumigate under the wooden sieve. After 35 minutes of fumigation, , take out the mixed raw materials for later use;

[0049] (2) Crushing treatment:

[0050] Put the mixed raw material after the fumigation treatment in step (1) into a cryogenic pulverizer for pulverization treatment, after pulverization treatment for 26 minutes, pass through a 60-mesh sieve for later use;

[0051] (3) Homogenization treatment:

[0052] Put the sieved powder and sterile water in step (2) into the micro-jet high-pressure homogenizer according to the weight-to-volume ratio of 1g:4.5mL, and then carry out homogeniz...

Embodiment 3

[0065] A preparation method of Phalaenopsis tissue culture rapid propagation medium, comprising the steps of:

[0066] (1) Fumigation treatment:

[0067] First weigh 15 parts of wax gourd rind, 10 parts of watermelon rind, 7 parts of amaranth stalk, 13 parts of cucumber rind, and 15 parts of pumpkin vine in corresponding weight parts and put them together on a wooden sieve, and then carry out steam fumigation under the wooden sieve. After 40 minutes of fumigation, , take out the mixed raw materials for later use;

[0068] (2) Crushing treatment:

[0069] Put the mixed raw material after the fumigation treatment in step (1) into a cryogenic pulverizer for pulverization treatment, after pulverization treatment for 29 minutes, pass through an 80-mesh sieve for later use;

[0070] (3) Homogenization treatment:

[0071] Put the powder sieved in step (2) and sterile water together into the micro-jet high-pressure homogenizer according to the weight-to-volume ratio of 1g:5mL, and ...

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Abstract

The invention discloses a method for preparing a rapid reproduction culture medium for tissue culture of phalaenopsis amabilis. The method comprises the following steps: (1) performing fumigation treatment; (2) performing crushing treatment; (3) performing homogenization treatment; (4) weighing raw materials; and (5) preparing a finished product. By adopting the culture medium finally prepared bythe method, rich nutrient elements can be provided for splitting and differentiation of phalaenopsis amabilis tissue, callus browning deterioration can be effectively prevented, the contamination rateof phalaenopsis amabilis tissue culture can be also greatly reduced, the callus splitting and differentiation capability can be improved, furthermore, the breeding rate of phalaenopsis amabilis tissue culture can be increased, and very high market popularization value can be made.

Description

technical field [0001] The invention belongs to the technical field of flower planting, and in particular relates to a preparation method of a phalaenopsis tissue culture rapid propagation medium. Background technique [0002] Phalaenopsis( Phalaenopsis amabilis ), also known as Phalaenopsis, is a tropical aerial orchid, a perennial herb. Phalaenopsis is a famous cut flower species and one of the four most commercially valuable ornamental tropical orchids in the world. Phalaenopsis has the reputation of "Queen of Orchids". With the increase of domestic market demand, the production prospect of Phalaenopsis will be better and better, and it has extremely high economic value. Because Phalaenopsis belongs to the uniaxial aerial orchid, the seeds are small, and the embryo and endosperm are not fully developed, so it is not suitable for sowing by traditional methods. Mass propagation of Phalaenopsis seedlings by tissue culture is a fast, convenient and effective way not limite...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 吴勇
Owner 安徽惠泽园苗木花卉种植有限公司
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