Method for identifying soft rot resistance of phalaenopsis amabilis

A technology for identification of resistance and soft rot, applied in the field of agricultural plant protection, to achieve the effect of scientific identification methods

Pending Publication Date: 2020-08-28
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present there is no method for approval of resistance identification about Phalaenopsis at home and abroad, therefore, it is particularly important to invent a method for accurately evaluating the resistance of varieties

Method used

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  • Method for identifying soft rot resistance of phalaenopsis amabilis
  • Method for identifying soft rot resistance of phalaenopsis amabilis
  • Method for identifying soft rot resistance of phalaenopsis amabilis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Isolation and preparation of pathogenic bacteria: Take Phalaenopsis soft rot samples from the orchid nursery of Guangdong Academy of Agricultural Sciences, disinfect according to routine, and then cut the tissue at the junction of disease and health into thin slices with a sterile knife and transfer them to NA medium for culture, after separation and purification , and verified by Koch’s law; cultivated on NA plates at 32°C to obtain pure culture of pathogenic bacteria; inoculated pathogenic strains in NA culture medium, cultivated at 32°C at 150r / min for 36h, and prepared with sterile water 10 6 -10 7 CFU / mL of bacterial suspension.

[0052] Plate disinfection and moisturizing: put the sterilized filter paper in a petri dish, spray sterile water until the filter paper is wet and set aside;

[0053] Species preparation for the test: select 18 Phalaenopsis varieties, prepare 5 leaves for each variety, and prepare 5 leaves with a size and diameter of 50mm-70mm. After cl...

Embodiment 2

[0074] Cultivation of pathogenic bacteria: samples of Phalaenopsis soft rot were taken from Shunde District, Guangdong Province, disinfected according to routine, and then the tissue at the junction of disease and health was cut into thin slices with a sterile knife and transferred to NA medium for cultivation, separated and purified, and passed Koch's law was verified and confirmed; cultured on NA plates at 32°C to obtain pure culture of pathogenic bacteria; cultured Phalaenopsis bacterial soft rot bacteria on NA plates at 32°C; inoculated pathogenic strains on NA culture medium, Cultivate at 30°C with a rotational speed of 150r / min for 40h, and prepare 10 6 CFU / mL of bacterial suspension.

[0075] Species preparation for the test: select 21 Phalaenopsis varieties, prepare 15 leaves for each variety, and prepare 15 leaves with a size and diameter of 50mm-70mm. After cleaning and drying, disinfect them with alcohol with a mass concentration of 75%, and use high-sensitivity sof...

Embodiment 3

[0096] Cultivation of pathogenic bacteria: Take Phalaenopsis soft rot samples from Conghua District, Guangzhou City, Guangdong Province, sterilize them according to routine, and then cut the tissue at the junction of disease and health into thin slices with a sterile knife and transfer them to NA medium for cultivation. After separation and purification, It was verified and confirmed by Koch's law; cultivated on NA plates at 32°C to obtain pure culture of pathogenic bacteria; took Phalaenopsis bacterial soft rot and cultured on NA plates at 30°C; inoculated the pathogenic strains in NA culture medium , cultivated at 30°C with a rotational speed of 130-200rpm / min for 30-36h, and prepared 10 7 CFU / mL of bacterial suspension.

[0097] Species preparation for the test: 13 Phalaenopsis varieties from the Guangdong Academy of Agricultural Sciences, 12 leaves for each variety, with a size and diameter of 50mm-70mm, cleaned and dried with alcohol with a mass concentration of 75%, and ...

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Abstract

The invention discloses and provides a method for identifying soft rot resistance of phalaenopsis amabilis. The method comprises the steps: scissoring middle-lower leaves of a phalaenopsis amabilis variety, wiping leaves and leaf stalk wounds with alcohol once, and carrying out natural air-drying; inoculating a NA culture solution with pathogenetic strains, and preparing a bacterial suspension byusing sterile water; densely and gently puncturing centers of the leaves with sterile pins, dripping the bacterial suspension to punctured positions, placing the leaves in magnetic discs for moisturized sealing and dark culture; observing and recording onset conditions of the leaves when water-spot-shaped lesions emerge from the leaves; and carrying out grading by average control efficiency, and evaluating infection resisting characteristics of the variety.

Description

technical field [0001] The invention relates to a method for identifying resistance to crop diseases and insect pests, in particular to a method for identifying resistance to Phalaenopsis soft rot, belonging to the field of agricultural plant protection. Background technique [0002] Phalaenopsis soft rot disease (phalaenopsis soft rot disease), caused by the Dickia bacterium Dickeyadieffenbachiae, is one of the main diseases of Phalaenopsis. It is widely distributed and occurs in all Phalaenopsis production areas across the country. After the onset, the pathogenic bacteria secrete pectinase, which dissolves the pectin in the cell wall, making the tissue soft and rotten, and spreads rapidly, causing serious loss of warp. Breeding of resistant varieties is the main means of controlling the disease. But at present, there is no method for approving the resistance identification of Phalaenopsis at home and abroad. Therefore, it is particularly important to invent a method for a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/01
CPCC12Q1/18G01N2333/195
Inventor 杨祁云林壁润沈会芳张景欣孙大元蒲小明
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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